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- Clone
- F38-2E2 (See other available formats)
- Regulatory Status
- RUO
- Workshop
- HCDM listed
- Other Names
- T cell immunoglobulin and mucin domain containing protein 3, hepatitis virus cellular receptor 2, CD366
- Isotype
- Mouse IgG1, κ
- Ave. Rating
- Submit a Review
- Product Citations
- publications
-
Th1-polarized human peripheral blood mononuclear cells were stained with CD4 FITC and anti-human CD366 (Tim-3, clone F38-2E2) PE (top) or mouse IgG1, κ PE (bottom). -
| Cat # | Size | Price | Quantity Check Availability | Save | ||
|---|---|---|---|---|---|---|
| 345009 | 100 µg | 216€ | ||||
| 345010 | 1 mg | 595€ | ||||
CD366 (Tim-3) is a transmembrane protein also known as T cell immunoglobulin and mucin domain containing protein-3. Tim-3 is expressed at high levels on activated T cells (preferentially on Th1 cells, monocytes/macrophages, and dendritic cells). Tim-3 has also been shown to exist as a soluble protein. Cells expressing Tim-3 are present at high levels in the CNS of animals at the onset of experimental autoimmune encephalomyelitis (EAE), a disease mediated by lymphocytes secreting Th1-like cytokines. Tim-3 has been proposed to inhibit Th1-mediated immune responses and promote immunological tolerance.
Product DetailsProduct Details
- Verified Reactivity
- Human
- Antibody Type
- Monoclonal
- Host Species
- Mouse
- Immunogen
- Human Tim-3 fusion protein
- Formulation
- 0.2 µm filtered in phosphate-buffered solution, pH 7.2, containing no preservative. Endotoxin level is <0.01 EU/µg of the protein (<0.001 ng/µg of the protein) as determined by the LAL test.
- Preparation
- The Ultra-LEAF™ (Low Endotoxin, Azide-Free) antibody was purified by affinity chromatography.
- Concentration
- The antibody is bottled at the concentration indicated on the vial, typically between 2 mg/mL and 3 mg/mL. Older lots may have also been bottled at 1 mg/mL. To obtain lot-specific concentration and expiration, please enter the lot number in our Certificate of Analysis online tool.
- Storage & Handling
- The antibody solution should be stored undiluted between 2°C and 8°C. This Ultra-LEAF™ solution contains no preservative; handle under aseptic conditions.
- Application
-
FC - Quality tested
Costim, Block - Reported in the literature, not verified in house - Recommended Usage
-
Each lot of this antibody is quality control tested by immunofluorescent staining with flow cytometric analysis. For flow cytometric staining, the suggested use of this reagent is ≤0.5 µg per million cells in 100 µl volume or 100 µl of whole blood. It is recommended that the reagent be titrated for optimal performance for each application.
- Application Notes
-
Additional reported applications (for relevant formats of this clone) include: costimulation1 (clone 2E2 has been shown to enhance T-cell receptor mediated activation and cytokine secretion) and blocking2,3.
-
Application References
(PubMed link indicates BioLegend citation) -
- Hastings WD, et al. 2009. Eur. J. Immunol. 39:2492. (Costim)
- Jones RB, et al. 2008. J. Exp. Med. 205:2763. (Block)
- Klibi J, et al 2009. Blood 113:1957. (FC, Block)
- Product Citations
-
- RRID
-
AB_11150398 (BioLegend Cat. No. 345009)
AB_11150780 (BioLegend Cat. No. 345010)
Antigen Details
- Structure
- Transmembrane protein containing immunoglobulin domain and mucin-like domain; can exist as a soluble form lacking mucin and transmembrane domains
- Distribution
-
Activated T cells, preferentially on Th1 cells, monocytes, dendritic cells
- Function
- Plays a role in regulating macrophage activation, T cell apoptosis and immune tolerance
- Ligand/Receptor
- Galectin-9
- Cell Type
- Dendritic cells, Monocytes, T cells, Th1, Tregs
- Biology Area
- Immunology, Inhibitory Molecules
- Molecular Family
- CD Molecules, Immune Checkpoint Receptors
- Antigen References
-
1. Hafler DA and Kuchroo V. 2008. J. Exp. Med. 205:2699.
2. Zhu C, et al. 2005. Nat. Immunol. 6:1245.
3. Wang F, et al. 2009. Immunobiology 214:342. - Gene ID
- 84868 View all products for this Gene ID
- UniProt
- View information about CD366 on UniProt.org
Related FAQs
- Do you guarantee that your antibodies are totally pathogen free?
-
BioLegend does not test for pathogens in-house aside from the GoInVivo™ product line. However, upon request, this can be tested on a custom basis with an outside, independent laboratory.
- Does BioLegend test each Ultra-LEAF™ antibody by functional assay?
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No, BioLegend does not test Ultra-LEAF™ antibodies by functional assays unless otherwise indicated. Due to the possible complexities and variations of uses of biofunctional antibodies in different assays and because of the large product portfolio, BioLegend does not currently perform functional assays as a routine QC for the antibodies. However, we do provide references in which the antibodies were used for functional assays and we do perform QC to verify the specificity and quality of the antibody based on our strict specification criteria.
- Does BioLegend test each Ultra-LEAF™ antibody for potential pathogens?
-
No, BioLegend does not test for pathogens in-house unless otherwise indicated. However, we can recommend an outside vendor to perform this testing as needed.
- Have you tested this Ultra-LEAF™ antibody for in vivo or in vitro applications?
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We don't test our antibodies for in vivo or in vitro applications unless otherwise indicated. Depending on the product, the TDS may describe literature supporting usage of a particular product for bioassay. It may be best to further consult the literature to find clone specific information.
Other Formats
View All CD366 Reagents Request Custom ConjugationCustomers Also Purchased
Compare Data Across All Formats
This data display is provided for general comparisons between formats.
Your actual data may vary due to variations in samples, target cells, instruments and their settings, staining conditions, and other factors.
If you need assistance with selecting the best format contact our expert technical support team.
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Purified anti-human CD366 (Tim-3)
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PE anti-human CD366 (Tim-3)
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Brilliant Violet 421™ anti-human CD366 (Tim-3)
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Ultra-LEAF™ Purified anti-human CD366 (Tim-3)
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APC anti-human CD366 (Tim-3)
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PE/Cyanine7 anti-human CD366 (Tim-3)
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PerCP/Cyanine5.5 anti-human CD366 (Tim-3)
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Brilliant Violet 605™ anti-human CD366 (Tim-3)
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FITC anti-human CD366 (Tim-3)
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Purified anti-human CD366 (Tim-3) (Maxpar® Ready)
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Brilliant Violet 711™ anti-human CD366 (Tim-3)
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APC/Cyanine7 anti-human CD366 (Tim-3)
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Brilliant Violet 785™ anti-human CD366 (Tim-3)
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Brilliant Violet 650™ anti-human CD366 (Tim-3)
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Brilliant Violet 510™ anti-human CD366 (Tim-3)
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PE/Dazzle™ 594 anti-human CD366 (Tim-3)
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GoInVivo™ Purified anti-human CD366 (Tim-3)
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APC/Fire™ 750 anti-human CD366 (Tim-3)
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Pacific Blue™ anti-human CD366 (Tim-3)
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Biotin anti-human CD366 (Tim-3)
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TotalSeq™-A0169 anti-human CD366 (Tim-3)
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TotalSeq™-C0169 anti-human CD366 (Tim-3)
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PE/Cyanine5 anti-human CD366 (Tim-3)
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TotalSeq™-B0169 anti-human CD366 (Tim-3)
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Brilliant Violet 750™ anti-human CD366 (Tim-3) Antibody
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TotalSeq™-D0169 anti-human CD366 (Tim-3)
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PE/Fire™ 810 anti-human CD366 (Tim-3)
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PE/Fire™ 640 anti-human CD366 (Tim-3)
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