Loading...

Ultra-LEAF™ Purified anti-HA.11 Epitope Tag Antibody

Pricing & Availability
Clone
16B12 (See other available formats)
Regulatory Status
RUO
Other Names
HA epitope tag, HA1, HA2, hemagglutinin, Hemagglutinin HA1 chain, Hemagglutinin HA2 chain, YPYDVPDYA, Hemagglutinin tag
Isotype
Mouse IgG1, κ
Ave. Rating
Submit a Review
Product Citations
publications
1_16B12_ULEAF_HAdot11_Antibody_WB_082417
Total cell lysate (15 µg protein) from CHO (lane 1) and CHO stably transfected with HA tag fused protein (lane 2) were resolved by electrophoresis (4-12% Bis-Tris gel), transferred to nitrocellulose, and probed with 1:1000 diluted (1 µg/mL), 1:4000 diluted (0.25 µg/mL) and 1:10000 diluted (0.1 µg/mL) purified anti-HA.11 Epitope Tag antibody (clone 16B12) (upper). Proteins were visualized by chemiluminescence detection using 1:3000 diluted HRP Goat anti-mouse IgG antibody. 1:2000 dilution of Direct-Blot™ HRP anti-β-actin antibody (clone 2F1-1) was used as a loading control (lower).
  • 1_16B12_ULEAF_HAdot11_Antibody_WB_082417
    Total cell lysate (15 µg protein) from CHO (lane 1) and CHO stably transfected with HA tag fused protein (lane 2) were resolved by electrophoresis (4-12% Bis-Tris gel), transferred to nitrocellulose, and probed with 1:1000 diluted (1 µg/mL), 1:4000 diluted (0.25 µg/mL) and 1:10000 diluted (0.1 µg/mL) purified anti-HA.11 Epitope Tag antibody (clone 16B12) (upper). Proteins were visualized by chemiluminescence detection using 1:3000 diluted HRP Goat anti-mouse IgG antibody. 1:2000 dilution of Direct-Blot™ HRP anti-β-actin antibody (clone 2F1-1) was used as a loading control (lower).
  • 2_16B12_PURE_HA-11_EpitopeTag_Antibody_120321.png
    Transfected RBL1 cells expressing the HA tag on the cell surface were stained with purified anti-HA.11 epitope Tag (clone 16B12) (filled histogram) or purified mouse IgG1, κ isotype control (open histogram), followed by APC conjugated goat-anti-mouse IgG.
Compare all formats
Cat # Size Price Quantity Check Availability Save
901521 100 µg 216€
Check Availability


Need larger quantities of this item?
Request Bulk Quote
901522 1 mg 572€
Check Availability


Need larger quantities of this item?
Request Bulk Quote
Description

The HA tag (hemagglutinin) is an amino acid sequence derived from the human influenza hemagglutinin surface glycoprotein, corresponding to amino acids 98-106.  It is commonly used as a tag to facilitate detection, isolation, and purification of proteins. The full amino acid sequence is: YPYDVPDYA.

Product Details
Technical Data Sheet (pdf)

Product Details

Antibody Type
Monoclonal
Host Species
Mouse
Immunogen
Monoclonal antibody HA.11 was raised against the twelve amino acid peptide CYPYDVPDYASL.
Formulation
0.2 µm filtered in phosphate-buffered solution, pH 7.2, containing no preservative.
Endotoxin Level
Less than 0.01 EU/µg of the protein (< 0.001 ng/µg of the protein) as determined by the LAL test.
Preparation
The Ultra-LEAF™ (Low Endotoxin, Azide-Free) antibody was purified by affinity chromatography.
Concentration
The antibody is bottled at the concentration indicated on the vial, typically between 2 mg/mL and 3 mg/mL. Older lots may have also been bottled at 1 mg/mL. To obtain lot-specific concentration and expiration, please enter the lot number in our Certificate of Analysis online tool.
Storage & Handling
The antibody solution should be stored undiluted between 2°C and 8°C. This Ultra-LEAF™ solution contains no preservative; handle under aseptic conditions.
Application

WB - Quality tested
FC - Verified
Purification - Reported in the literature, not verified in house
Recommended Usage

Each lot of this antibody is quality control tested by Western blotting. For Western blotting, the suggested use of this reagent is 1:2000-1:20000 diluted (0.05 - 0.5 µg/mL). It is recommended that the reagent be titrated for optimal performance for each application.

Application Notes

Additional tested and reported applications of the 16B12 clone for the relevant formats include: western blot (WB), immunocytochemistry (ICC), immunoprecipitation (IP), and flow cytometry (FC).

*Our Posi-Tag Control Protein (931301) can be used as a helpful positive control for this antibody.

This second-generation HA antibody is an excellent substitute for the 12CA5 monoclonal antibody. The HA.11 antibody recognizes the influenza hemagglutinin epitope (YPYDVPDYA) which has been used extensively as a general epitope tag in expression vectors. The extreme specificity of the antibody allows unambiguous identification and quantitative analysis of the tagged protein. The HA.11 antibody recognizes HA epitopes located in the middle of protein sequences as well as at the N- or C-terminus.

Application References

(PubMed link indicates BioLegend citation)
  1. Kim JY, et al. 2003. J Neurosci. 23:5561. (IP, WB)
  2. Helliwell SB, et al. 2001. J Cell Biol. 153:649. (WB)
  3. Bennett BD, et al. 2000. J Biol Chem. 275:37712. (IF, IP, WB)
  4. Royer Y, et al. 2005. J. Biol. Chem. 29:27251. (FC)
  5. Smith BA, et al. 2012. Genes Cancer. 3:550. (IHC) PubMed
  6. Hogarth C, et al. 2015. Biol Reprod. 93:19. PubMed
  7. Görtz D, et al. 2015. Sci Rep. 5:14685. PubMed
  8. Wilson C, et al. 2015. PLoS One. 10:0139579. PubMed
  9. Smith B, et al. 2012. Genes Cancer. 3:550-563. PubMed
  10. Liu Z, et al. 2016. Nature. 530:98-102. PubMed
  11. Thoms M, et al. 2016. Nucleic Acids Res. 44:926-39. PubMed
  12. Kim Y, et al. 2016. Nat Commun. 7:10347. PubMed
  13. Rodríguez-Escudero M, et al. 2016. PLoS One. 11:0148032. PubMed
  14. Lehmann W, et al. 2016. Nat Commun. 7:10498. PubMed
  15. Testoni E, et al. 2016. EMBO Mol Med. 8: 105-16. PubMed
  16. Padilla S, et al. 2016. Nat Neurosci. 10.1038/nn.4274. PubMed
  17. Martins J, et al. 2016. J Cell Sci. 129:1271-82. PubMed
  18. Matak P, et al. 2016. Proc Natl Acad Sci U S A. 113:3428-35. PubMed
  19. Starokadomskyy P, et al. 2016. Nat Immunol. 17:495-504. PubMed
  20. Mitxelena J, et al. 2016. Nucleic Acids Res. 44:5557-70. PubMed
  21. Thongthip S, et al. 2016. Genes Dev. 30:645-59. PubMed
  22. Aaes T, et al. 2016. Cell Rep. 15:274-87. PubMed
  23. Hodge C, et al. 2016. J Biol Chem. 291:9396-9410. PubMed
  24. Alagramam K, et al. 2016. Nat Chem Biol. 12:444-51. PubMed
  25. Veit G, et al. 2016. PLoS Biol. 14:1002462. PubMed
  26. Lee B, et al. 2016. Development. 143:1721-31. PubMed
  27. Douchi D, et al. 2016. Plant Cell. 28:1182-99. PubMed
  28. Avgousti D, et al. 2016. Nature. 535:173-77. PubMed
  29. Shin H, et al. 2015. Nature. 534:553-7. PubMed
  30. Gross G, et al. 2016. Nat Methods. 10.1038/nmeth.3894. PubMed
  31. Dick M, et al. 2016. Nat Commun. 7:11929. PubMed
  32. Aldrin-Kirk P, et al. 2016. Neuron. 90:955-68. PubMed
  33. Fan R, et al. 2016. Nat Med. 22:780-91. PubMed
  34. Rowald K, et al. 2016. Cell Rep. 15:2679-91. PubMed
  35. Rampal R, Awasthi A, Ahuja V 2016. Development. 143:2334-43. PubMed
  36. Faden F, et al. 2016. Nat Commun. 7:12202. PubMed
  37. Lawson C, et al. 2016. Cancer Res. 76: 3826-37. PubMed
  38. Szargel R, et al. 2016. Hum Mol Genet. 10.1093/hmg/ddw189.. PubMed
  39. Damez-Werno D, et al. 2016. Proc Natl Acad Sci U S A. 113: 9623-28. PubMed
  40. Su P, et al. 2016. J Immunol. 197: 1054-64. PubMed
  41. Morozumi Y, et al. 2016. J Mol Cell Biol. 8:349-62. PubMed
  42. Miura Y, et al. 2016. Biochem J. 473:2591-602. PubMed
  43. Pashkova N, et al. 2016. Cell Rep. 17:303-15. PubMed
  44. Sanchez M, et al. 2016. J Biol Chem. 291:19760-73. PubMed
  45. Hwang J, Lee J, Pallas D 2016. J Biol Chem. 291:21008-19. PubMed
  46. Jiménez-Canino R, et al. 2016. J Biol Chem. 291:19068-78. PubMed
  47. Barquilla A, et al. 2016. Mol Biol Cell. 27:2757-70. PubMed
  48. Yadav S, et al. 2016. Sci Rep. 6:34100. PubMed
  49. Hashimoto A, et al. 2016. Oncogenesis. 0.388194444. PubMed
  50. Guirouilh-Barbat J, et al. 2016. PLoS Genet. 12:e1006230. PubMed
  51. Gómez-H L, et al. 2016. Nat Commun. 7:13298. PubMed
  52. Rademacher N, et al. 2016. Sci Rep. 6:35283. PubMed
  53. Lin Z, et al. 2016. Nat Genet. 10.1038/ng.3701. PubMed
  54. Kaczmarska Z, et al. 2016. Nat Chem Biol. 10.1038/nchembio.2217. PubMed
  55. Zee B, et al. 2016. PLoS One. 11:e0163820. PubMed
  56. Despras E, et al. 2016. Nat Commun. 7:13326. PubMed
  57. Zhang H, et al. 2016. J Neurosci. 36:11837-50. PubMed
  58. Zhang M, et al. 2016. Cell Res. 26:1302-19. PubMed
  59. Baehr C, et al. 2016. J Biol Chem. 291:26875-85. PubMed
  60. Boehm E, et al. 2016. J Biol Chem. 291:25877-87. PubMed
  61. Gail Kilroy, et al. 2016. J Biol Chem. 291:27289-97. PubMed
  62. Hongchen Cai, Aimin Liu 2016. Proc Natl Acad Sci U S A. 113:14751-6. PubMed
  63. Tam K, et al. 2016. MBio. 7:e02024-16. PubMed
  64. Hanke L, et al. 2016. MBio. 7(6). pii: e01569-16. PubMed
  65. Stoeber M, et al. 2016. Proc Natl Acad Sci U S A. 113(50):E8069-E8078. PubMed
  66. Gu Q, et al. 2016. J Virol. 90:10545-57. PubMed
  67. Ramachandran S, et al. 2016. J Biol Chem. 291: 25489-504. PubMed
  68. Kaufmann T, et al. 2016. J Cell Sci. 129:4607-21. PubMed
  69. Piwko W, et al. 2016. EMBO J. 35:2584-601. PubMed
  70. Matsuo Y, et al. 2016. J Cell Sci. 129:4592-606. PubMed
  71. Athmer J, et al. 2017. MBio. 10.1128/mBio.02320-16. PubMed
  72. Gao L, et al. 2017. J Cell Sci. 130:396-405. PubMed
  73. Gong Y, et al. 2017. Genes Dev. 31:46-58. PubMed
  74. K Kataoka, K Mochizuki 2017. J Cell Sci. 130:480-9. PubMed
  75. Liszczak G, et al. 2017. Proc Natl Acad Sci U S A. 114:681-6. PubMed
  76. Haggie P, et al. 2017. J Biol Chem. 292:771-85. PubMed
  77. T Taetzsch, et al. 2017. J Neurosci. 37:70-82. PubMed
  78. Yagita Y, et al. 2017. J Biol Chem. 292:691-705. PubMed
  79. Chong Jiang, et al. 2017. J Biol Chem. 292:3137-45. PubMed
  80. Maeda R, et al. 2017. J Biol Chem. 292:3201-12. PubMed
  81. Davis RB, et al. 2017. JCI Insight. 2:e92465. PubMed
  82. Daniel JA, et al. 2017. Elife. 6:e26338. PubMed
  83. Oishi A, et al. 2017. Sci Rep. 7:8990. PubMed
Product Citations
  1. Jung Y, et al. 2021. Nat Commun. 12:3872. PubMed
RRID
AB_2716058 (BioLegend Cat. No. 901521)
AB_2716059 (BioLegend Cat. No. 901522)

Antigen Details

Biology Area
Cell Biology
Gene ID
NA
UniProt
View information about HA.11 on UniProt.org

Related FAQs

Do you guarantee that your antibodies are totally pathogen free?

BioLegend does not test for pathogens in-house aside from the GoInVivo™ product line. However, upon request, this can be tested on a custom basis with an outside, independent laboratory.

Does BioLegend test each Ultra-LEAF™ antibody by functional assay?

No, BioLegend does not test Ultra-LEAF™ antibodies by functional assays unless otherwise indicated. Due to the possible complexities and variations of uses of biofunctional antibodies in different assays and because of the large product portfolio, BioLegend does not currently perform functional assays as a routine QC for the antibodies. However, we do provide references in which the antibodies were used for functional assays and we do perform QC to verify the specificity and quality of the antibody based on our strict specification criteria.

Does BioLegend test each Ultra-LEAF™ antibody for potential pathogens?

No, BioLegend does not test for pathogens in-house unless otherwise indicated.  However, we can recommend an outside vendor to perform this testing as needed.

Have you tested this Ultra-LEAF™ antibody for in vivo or in vitro applications?

We don't test our antibodies for in vivo or in vitro applications unless otherwise indicated. Depending on the product, the TDS may describe literature supporting usage of a particular product for bioassay. It may be best to further consult the literature to find clone specific information.

Go To Top Version: 3    Revision Date: 12/03/2021

For Research Use Only. Not for diagnostic or therapeutic use.

 

This product is supplied subject to the terms and conditions, including the limited license, located at www.biolegend.com/terms) ("Terms") and may be used only as provided in the Terms. Without limiting the foregoing, BioLegend products may not be used for any Commercial Purpose as defined in the Terms, resold in any form, used in manufacturing, or reverse engineered, sequenced, or otherwise studied or used to learn its design or composition without express written approval of BioLegend. Regardless of the information given in this document, user is solely responsible for determining any license requirements necessary for user’s intended use and assumes all risk and liability arising from use of the product. BioLegend is not responsible for patent infringement or any other risks or liabilities whatsoever resulting from the use of its products.

 

BioLegend, the BioLegend logo, and all other trademarks are property of BioLegend, Inc. or their respective owners, and all rights are reserved.

 

8999 BioLegend Way, San Diego, CA 92121 www.biolegend.com
Toll-Free Phone: 1-877-Bio-Legend (246-5343) Phone: (858) 768-5800 Fax: (877) 455-9587

This data display is provided for general comparisons between formats.
Your actual data may vary due to variations in samples, target cells, instruments and their settings, staining conditions, and other factors.
If you need assistance with selecting the best format contact our expert technical support team.

ProductsHere
Login / Register
Remember me
Forgot your password? Reset password?
Create an Account