- Ber-ACT8 (See other available formats)
- Regulatory Status
- V A067
- Other Names
- Integrin alpha E (ITGAE)
- Mouse IgG1, κ
- Barcode Sequence
- Ave. Rating
- Submit a Review
- Product Citations
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CD103 is a type I transmembrane glycoprotein also known as αE integrin, integrin αIEL chain, and human mucosal lymphocyte antigen 1. It belongs to the integrin family and is primarily found on intestinal intraepithelial lymphocytes (IEL). CD103 is also expressed on a subpopulation of lamina propria T cells, epithelial dendritic cells, lamina propria-derived dendritic cells, and a small subset of peripheral lymphocytes. Treg cells express high level of CD103. Hairy cell leukemia has also been shown to express CD103. The expression of CD103 on lymphocytes can be induced upon activation and TGF-β stimulation. In association with integrin β7, CD103 is expressed as an αE/β7 heterodimer. Mature CD103 protein can be cleaved into 2 chains, a 150 kD (C-terminal) chain and a 25 kD (N-terminal) chain, which remain linked by disulfide bonds. CD103 binds to E-cadherin and mediates homing of lymphocytes to the intestinal epithelium.Product Details
- Verified Reactivity
- Reported Reactivity
- Antibody Type
- Host Species
- HTLV-1 induced human T cell line MAPS16
- Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide and 1 mM EDTA
- The antibody was purified by chromatography and conjugated with TotalSeq™-D oligomer under optimal conditions.
- 0.5 mg/mL
- Storage & Handling
- The antibody solution should be stored undiluted between 2°C and 8°C. Do not freeze.
PG - Quality tested
- Recommended Usage
Each lot of this antibody is quality control tested by immunofluorescent staining with flow cytometric analysis and the oligomer sequence is confirmed by sequencing. TotalSeq™-D antibodies are compatible with Mission Bio’s Tapestri Single-Cell Sequencing Platform for simultaneous detection of DNA and Protein.
To maximize performance, it is strongly recommended that the reagent be titrated for each application, and that you centrifuge the antibody dilution before adding to the cells at 14,000xg at 2 - 8°C for 10 minutes. Carefully pipette out the liquid avoiding the bottom of the tube and add to the cell suspension. For Proteogenomics analysis, the suggested starting amount of this reagent for titration is ≤ 1.0 µg per million cells in 100 µL volume. Refer to the corresponding TotalSeq™ protocol for specific staining instructions.
Buyer is solely responsible for determining whether Buyer has all intellectual property rights that are necessary for Buyer's intended uses of the BioLegend TotalSeq™ products. For example, for any technology platform Buyer uses with TotalSeq™, it is Buyer's sole responsibility to determine whether it has all necessary third party intellectual property rights to use that platform and TotalSeq™ with that platform.
- Application Notes
Additional reported applications (for the relevant formats) include: Western Blotting1, immunoprecipitation1, and immunohistochemical staining of frozen tissue sections1.
- Additional Product Notes
TotalSeq™-D reagents are designed to profile protein expression at single cell level. The Mission Bio Tapestri platform and sequencer (e.g. Illumina analyzers) are required. Please contact technical support for more information, or visit biolegend.com/totalseq/single-cell-dna
The barcode flanking sequences are CGAGATGACTACGCTACTCATGG (PCR handle), and GAGCCGATCTAGTATCTCAGT*C*G (capture sequence). * indicates a phosphorothioated bond, to prevent nuclease degradation.
View more applications data for this product in our Application Technical Notes.
(PubMed link indicates BioLegend citation)
- Kruschwitz M, et al. 1991. J. Clin. Pathol. 44:636. (WB, IP, IHC-F)
- Yoshino N, et al. 2000. Exp. Anim. (Tokyo) 49:97. (FC)
AB_2922567 (BioLegend Cat. No. 350241)
- Type I transmembrane glycoprotein, integrin family; can be cleaved into 150 kD and 25 kD chains; associated with β7 integrin
Majority of intestinal intraepithelial lymphocytes (IEL), subpopulation of lamina propria T cells, epithelial dendritic cells, small subset of peripheral lymphocytes, Treg cells; expressed on hairy cell leukemia
- Retention and activation of CD103+ lymphocytes in the intestinal epithelium, regulation of tissue-specific T cell homing
- Cell Targets
- Integrin β7
- Cell Type
- Dendritic cells, Lymphocytes, T cells, Tregs
- Biology Area
- Cell Biology, Immunology, Neuroscience, Synaptic Biology
- Molecular Family
- Adhesion Molecules, CD Molecules
- Antigen References
1. Parker CM, et al. 1992. P. Natl. Acad. Sci. USA 89:1924.
2. Kruschwitz M, et al. 1991. J. Clin. Pathol. 44:636.
3. Schon MP, et al. 1999. J. Immunol. 162:6641.
4. Shaw SK, et al. 1994. J. Biol. Chem. 269:6016.
- Gene ID
- 3682 View all products for this Gene ID
- View information about CD103 on UniProt.org
Compare Data Across All Formats
This data display is provided for general comparisons between formats.
Your actual data may vary due to variations in samples, target cells, instruments and their settings, staining conditions, and other factors.
If you need assistance with selecting the best format contact our expert technical support team.
Purified anti-human CD103 (Integrin αE)
FITC anti-human CD103 (Integrin αE)
PE anti-human CD103 (Integrin αE)
Alexa Fluor® 488 anti-human CD103 (Integrin αE)
Alexa Fluor® 647 anti-human CD103 (Integrin αE)
PE/Cyanine7 anti-human CD103 (Integrin αE)
Brilliant Violet 421™ anti-human CD103 (Integrin αE)
APC anti-human CD103 (Integrin αE)
Brilliant Violet 605™ anti-human CD103 (Integrin αE)
Biotin anti-human CD103 (Integrin αE)
Brilliant Violet 711™ anti-human CD103 (Integrin αE)
PE/Dazzle™ 594 anti-human CD103 (Integrin αE)
PerCP/Cyanine5.5 anti-human CD103 (Integrin αE)
Brilliant Violet 785™ anti-human CD103 (Integrin αE)
APC/Cyanine7 anti-human CD103 (Integrin αE)
TotalSeq™-A0145 anti-human CD103 (Integrin αE)
TotalSeq™-C0145 anti-human CD103 (Integrin αE)
TotalSeq™-B0145 anti-human CD103 (Integrin αE)
APC/Fire™ 750 anti-human CD103 (Integrin αE)
PE/Fire™ 700 anti-human CD103 (Integrin αE) Antibody
TotalSeq™-D0145 anti-human CD103 (Integrin αE)
PE/Fire™ 640 anti-human CD103 (Integrin αE)
PE anti-human CD103
FITC anti-human CD103