Recombinant Mouse SCF (carrier-free)

Pricing & Availability
Regulatory Status
RUO
Other Names
Kit Ligand, KITLG, Mast cell growth factor, MGF, Steel factor, SLF, Stem Cell Factor
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Product Citations
publications
Mouse SCP 012110
TF-1 cell proliferation induced by mouse SCF
  • Mouse SCP 012110
    TF-1 cell proliferation induced by mouse SCF
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579708 500 µg 1336€
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579702 10 µg 165€
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579704 25 µg 306€
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579706 100 µg 680€
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Description

SCF (KITL) is a hematopoietic growth factor, and it can synergize with a number of other cytokines to stimulate growth of hemopoietic progenitors in vitro and stimulates blood cell production in vivo. SCF is encoded by Sl ('steel'), a gene critical to the development of several distinct cell lineages during embryonic life. KITL was identified as a soluble protein; nevertheless, the predicted amino acid sequence indicates that it is an integral transmembrane protein. KITL is generated by proteolytic cleavage from a transmembrane precursor. Two splice variants have been described for KITL, and proteolytic processing of both transmembrane protein products occurs on the cell surface. The binding of KITL, induces the dimerization of the KIT molecule, followed by a change in the configuration of the intracellular domain and the autophosphorylation of the receptor, opening several docking sites for signal transduction molecules. Dysregulation of SCF–KI signaling and gain-of-function KIT mutations contribute to the genesis of many cancers, like acute myeloid leukemia, gastrointestinal stromal tumors, and mastocytosis.

Product Details
Technical Data Sheet (pdf)

Product Details

Source
Mouse SCF, amino acids Lys26-Ala189 (Accession# M59915) was expressed in E. coli.
Molecular Mass
The 165 amino acid N-terminal methionylated recombinant protein has a predicted molecular mass of 18298.0 Da. The DTT-reduced protein migrates at approximately 18 kDa by SDS-PAGE. The non-reduced protein migrates at approximately 14 kDa by SDS-PAGE.
Purity
>98%, as determined by Coomassie stained SDS-PAGE.
Formulation
0.22 µm filtered protein solution is in 10 mM NaH2PO4 pH 7.2, 0.15M NaCl.
Endotoxin Level
Less than 0.01ng per µg cytokine as determined by the LAL method.
Concentration
10 and 25 µg sizes are bottled at 200 µg/mL. 100 µg size and larger sizes are lot-specific and bottled at the concentration indicated on the vial. To obtain lot-specific concentration and expiration, please enter the lot number in our Certificate of Analysis online tool.
Storage & Handling
Unopened vial can be stored between 2°C and 8°C for up to 2 weeks, at -20°C for up to six months, or at -70°C or colder until the expiration date. For maximum results, quick spin vial prior to opening. The protein can be aliquoted and stored at -20°C or colder. Stock solutions can also be prepared at 50 - 100 µg/mL in appropriate sterile buffer, carrier protein such as 0.2 - 1% BSA or HSA can be added when preparing the stock solution. Aliquots can be stored between 2°C and 8°C for up to one week and stored at -20°C or colder for up to 3 months. Avoid repeated freeze/thaw cycles.
Activity
ED50 = 5 - 20 ng/ml, corresponding to a specific activity of 0.5 - 2.0 x 105 units/mg, as determined by the dose dependent stimulation of TF-1 cell proliferation.
Application

Bioassay

Application Notes

BioLegend carrier-free recombinant proteins provided in liquid format are shipped on blue-ice. Our comparison testing data indicates that when handled and stored as recommended, the liquid format has equal or better stability and shelf-life compared to commercially available lyophilized proteins after reconstitution. Our liquid proteins are verified in-house to maintain activity after shipping on blue ice and are backed by our 100% satisfaction guarantee. If you have any concerns, contact us at tech@biolegend.com.

Product Citations
  1. Paris J et al. 2019. Cell Stem Cell. 25(1):137-148 . PubMed
  2. Bachran C, et al. 2017. Sci Rep.. 10.1038/s41598-017-17948-0. PubMed
  3. Xiong W, et al. 2021. Int J Mol Sci. 22:. PubMed
  4. Caslin H, et al. 2017. Cell Immunol. 10.1016/j.cellimm.2017.04.013. PubMed
  5. Bagayoko S, et al. 2021. PLoS Pathog. 17:e1009927. PubMed
  6. Chen X et al. 2017. Cell stem cell. 21(6):747-760 . PubMed
  7. Oherle K, et al. 2020. Immunity. 275:52. PubMed
  8. Kinoshita M, et al. 2020. Cell Stem Cell. . PubMed
  9. Walker JA, et al. 2020. Immunity. 51(1):104-118. PubMed

Antigen Details

Structure
Cytokine
Distribution

SCF is widely exppressed

Function
SCF has pleiotropic functions. It is involved in melanogenesis, gametogenesis, hematopoiesis and brain angiogenesis.
Interaction
The KIT receptor tyrosine kinase (RTK) is expressed in hematopoietic stem cells (multipotent hematopoietic stem cells, progenitors committed to myeloid and/or erythroid lineages, and T and B cell precursors), mast cells, and acute myeloid leukemia cells.
Ligand/Receptor
SCF binds to the extracellular domains of the KIT receptor tyrosine kinase.
Cell Type
Embryonic Stem Cells, Hematopoietic stem and progenitors
Biology Area
Angiogenesis, Cell Biology, Immunology, Signal Transduction, Stem Cells
Molecular Family
Cytokines/Chemokines, Growth Factors
Antigen References

1. Sun L, et al. 2006 Cancer Cell 9:287-300.
2. Magnol, et al. 2007 BMC Dev Biol. 7:81.
3. Liu H, et al. 2007 EMBO J 26:891-901.
4. Agosti V, et al. 2004 J. Exp. Med. 199:867-878.
5. Huang L el al.1992 Mol Cell Biol 3:349-362.
6. Kitamura T, et al. 1989 J. Cell Physiol. 140:323-334.

Gene ID
17311 View all products for this Gene ID
UniProt
View information about SCF on UniProt.org

Related FAQs

Why choose BioLegend recombinant proteins?

     • Each lot of product is quality-tested for bioactivity as indicated on the data sheet.
     • Greater than 95% Purity or higher, tested on every lot of product.
     • 100% Satisfaction Guarantee for quality performance, stability, and consistency.
     • Ready-to-use liquid format saves time and reduces challenges associated with reconstitution.
     • Bulk and customization available. Contact us.
     • Learn more about our Recombinant Proteins.

How does the activity of your recombinant proteins compare to competitors?

We quality control each and every lot of recombinant protein. Not only do we check its bioactivity, but we also compare it against other commercially available recombinant proteins. We make sure each recombinant protein’s activity is at least as good as or better than the competition’s. In order to provide you with the best possible product, we ensure that our testing process is rigorous and thorough. If you’re curious and eager to make the switch to BioLegend recombinants, contact your sales representative today!

What is the specific activity or ED50 of my recombinant protein?

The specific activity range of the protein is indicated on the product datasheets. Because the exact activity values on a per unit basis can largely fluctuate depending on a number of factors, including the nature of the assay, cell density, age of cells/passage number, culture media used, and end user technique, the specific activity is best defined as a range and we guarantee the specific activity of all our lots will be within the range indicated on the datasheet. Please note this only applies to recombinants labeled for use in bioassays. ELISA standard recombinant proteins are not recommended for bioassay usage as they are not tested for these applications.

Have your recombinants been tested for stability?

Our testing shows that the recombinant proteins are able to withstand room temperature for a week without losing activity. In addition the recombinant proteins were also found to withstand four cycles of freeze and thaw without losing activity.

Does specific activity of a recombinant protein vary between lots?

Specific activity will vary for each lot and for the type of experiment that is done to validate it, but all passed lots will have activity within the established ED50 range for the product and we guarantee that our products will have lot-to-lot consistency. Please conduct an experiment-specific validation to find the optimal ED50 for your system.

How do you convert activity as an ED50 in ng/ml to a specific activity in Units/mg?

Use formula Specific activity (Units/mg) = 10^6/ ED50 (ng/mL)

Go To Top Version: 4    Revision Date: 03/28/2024

For Research Use Only. Not for diagnostic or therapeutic use.

 

This product is supplied subject to the terms and conditions, including the limited license, located at www.biolegend.com/terms) ("Terms") and may be used only as provided in the Terms. Without limiting the foregoing, BioLegend products may not be used for any Commercial Purpose as defined in the Terms, resold in any form, used in manufacturing, or reverse engineered, sequenced, or otherwise studied or used to learn its design or composition without express written approval of BioLegend. Regardless of the information given in this document, user is solely responsible for determining any license requirements necessary for user’s intended use and assumes all risk and liability arising from use of the product. BioLegend is not responsible for patent infringement or any other risks or liabilities whatsoever resulting from the use of its products.

 

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