Recombinant Mouse CCL11 (ELISA Std.)

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Other Names
Eotaxin, eotaxin-1, small inducible cytokine subfamily A, member 11 (SCYA11)
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Mouse_CCL11_RECOM_ELISA_032317
  • Mouse_CCL11_RECOM_ELISA_032317
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564501 4 pack 64€
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Description

CCL11 is a member of the CC chemokine family and was originally identified as a chemoattractant for eosinophils. In response to allergens, CCL11, which participates in a variety of allergic diseases, promotes accumulation of eosinophils. In addition, CCL11 facilitates proliferation and metastasis of tumor cells expressing the CCL11 receptor, CCR3. CCL11 has been shown to induce angiogenic responses by CCR3-positive microvascular endothelial cells.

Product Details
Technical Data Sheet (pdf)

Product Details

Reactivity
Mouse
Source
Mouse CCL11 (amino acids His24-Pro97, Accession# NM_011330) was expressed in E. coli.
Molecular Mass
The 74 amino acid recombinant protein has a predicted molecular mass of approximately 8.4 kD. The DTT-reduced protein migrates at approximately 12 kD and non-reduced protein migrates at approximately 14 kD by SDS-PAGE. The N-terminal amino acid is Histidine.
Purity
>95%, as determined by Coomassie stained SDS-PAGE.
Formulation
Lyophilized in sterile-filtered PBS, pH 7.2, containing 1% BSA, 0.09% sodium azide, and protease inhibitors.
Preparation
ELISA Standard 4-Pack contains four vials of recombinant mouse CCL11 at >5 ng per vial. Recommended for ELISA application only. Standards are calibrated against a BioLegend Master Calibrator.
Concentration
≥ 5ng/vial
Storage & Handling
Upon receipt, store unopened vials between 2°C and 8°C immediately and use within 12 months from date of receipt. Prior to use, reconstitute the lyophilized powder with 0.2 ml of PBS containing a carrier protein (e.g., 1% BSA, protease free), pH 7.4. Re-cap vial, vortex. Allow the reconstituted standard to sit at room temperature for 15 minutes, vortex again to mix completely. The reconstituted standard stock solution can be aliquoted into polypropylene vials and stored at -70°C for up to one month. Do not re-use diluted standards. Use a manual defrost freezer and avoid repeated freeze thaw cycles.
Application

ELISA - Quality tested

Recommended Usage

Each lot of this protein is quality control tested by ELISA. For use as an ELISA standard, a standard curve comprised of doubling dilutions from 500 pg/mL to 7.81 pg/mL is suggested. It is recommended that the reagent be titrated for optimal performance.

Application Notes

This CCL11 protein is useful as a standard for a mouse CCL11 sandwich ELISA, using unlabeled L403H11 antibody as capture and biotinylated Poly5222 antibody as detection.

Antigen Details

Structure
Chemokine
Distribution

Epithelial cells, smooth muscle cells, dendritic cells, and fibroblasts

Function
CCL11 selectively recruits eosinophils through binding to the chemokine receptor CCR3 during allergic response. CCL11 production is upregulated in response to several proinflammatory cytokines, such as IL-4, IL-13, TNF-α, and IFN-α, produced by mast cells and Th2 lymphocytes. CCL11 has been reported to stimulate eosinophil release from bone marrow, resulting in an elevated level of circulating eosinophils.
Interaction
Eosinophils
Ligand/Receptor
CCR3
Biology Area
Angiogenesis, Cell Biology, Immunology, Neuroinflammation, Neuroscience
Molecular Family
Cytokines/Chemokines
Antigen References

1. Conroy DM, et al. 2001. Respir. Res. 2:150.
2. Levina V, et al. 2009. Clin. Cancer Res. 15:2647.
3. Salcedo R, et al. 2001. J. Immunol. 166:7571.
4. Kaehler J, et al. 2006. J. Investig. Med. 54:446.
5. Hadjicharalambous C, et al. 2004. J. Allergy Clin. Immunol. 113:657.
6. Crump MP, et al. 1998. J. Biol. Chem. 273:22471.

Gene ID
20292 View all products for this Gene ID
UniProt
View information about CCL11 on UniProt.org

Related FAQs

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