Recombinant Human Vitronectin (carrier-free)

Pricing & Availability
Regulatory Status
RUO
Other Names
VTN, VN, Serum-spreading factor, Complement S-protein, Somatomedin B, V75
Ave. Rating
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Product Citations
publications
Vitronectin_Human_RECOM_CF_BA_062716
Immobilized Human Vitronectin induces adhesion of HUVEC in a dose dependent manner. ED50 for this effect is 25 - 100 ng/mL.
  • Vitronectin_Human_RECOM_CF_BA_062716
    Immobilized Human Vitronectin induces adhesion of HUVEC in a dose dependent manner. ED50 for this effect is 25 - 100 ng/mL.
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757706 100 µg 72€
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757708 500 µg 179€
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Description

Vitronectin was first described as a serum-spreading factor that supported the attachment and spreading of cells onto glass. Vitronectin is a large glycoprotein found in blood and the extracellular matrix. It is a multifunctional protein that mediates cell-to-substrate adhesion, inhibits the cytolytic action of the terminal complement cascade in vitro, and binds to several serine protease inhibitors of the serpin family. Vitronectin is mainly synthesized in the liver and circulates primarily in monomeric form, but can undergo conformational change to a structure that forms disulfide linked multimers. The vitronectin molecule contains Somatomedin-B domain (SMB), four hemopexin domains, and the carboxyl-terminal end which has multiple sites and functions. The SMB domain mediates interaction with Serpine1/PAI1 and the heparin-binding domain at the carboxyl-terminal end mediates interaction with insulin. The carboxyl-terminal end also contains a plasminogen binding site, a glycosaminoglycan binding site, and a second PAI-1 binding site. The heparin binding site also mediates complement factor C7, C8, or C9 binding. Vitronectin’s ablility to bind platelet glycoproteins and mediate platelet adhesion and aggregation at sites of vascular injuries has made it an important mediator in the pathogenesis of coronary atherosclerosis.

Product Details
Technical Data Sheet (pdf)

Product Details

Source
Human Vitronectin, amino acids Asp20-Leu478 (Accession# AAH05046.1), was expressed in HEK293.
Molecular Mass
The 459 amino acid recombinant protein has a predicted molecular mass of approximately 52.3 kD. The protein migrates at an apparent molecular weight of 75 kD by SDS-PAGE under reducing conditions. The predicted N-terminal amino acid is Asp.
Purity
>95%, as determined by SDS-PAGE gel and HPLC analysis.
Formulation
Lyophilized from 0.2 µm filtered protein solution in 20 mM Sodium Phosphate, pH 7.5.
Endotoxin Level
Less than 1 EU per µg protein as determined by the LAL method.
Storage & Handling
Unopened vial can be stored at -20°C or -70°C. For maximum results, quick spin vial prior to opening. Reconstitute in water to a concentration of 0.1-0.5 mg/ml. Do not vortex. It is recommended to further dilute in a buffer containing a carrier protein such as 0.1% BSA and store working aliquots at -20°C to -80°C. Avoid repeated freeze/thaw cycles.
Activity
ED50 = 25 - 100 ng/mL as measured by the ability of immobilized protein to support the adhesion of HUVEC.
Application

Bioassay

Product Citations
  1. Fox CR, et al. 2021. Viruses. 14:. PubMed

Antigen Details

Structure
Monomer and Multimer.
Distribution
Mainly synthesized in the liver, high concentration in vasculature, and is found in most tissues.
Function
Mediates cell-to-substrate adhesion, inhibits the cytolytic action of the terminal complement cascade in vitro, and binds to several serine protease inhibitors of the serpin family.
Interaction
Interacts with SERPINE1/PAI1, insulin, C1QBP, and extracelluar matrix.
Ligand/Receptor
SERPINE1/PAI1, insulin, C1QBP, and Integrins.
Bioactivity
Measured by the ability of immobilized human Vitronectin to induce adhesion of HUVEC.
Cell Type
Embryonic Stem Cells
Biology Area
Complement, Stem Cells
Antigen References

1. Jenne D, et al. 1985. EMBO J. 4:3153-3157.
2. Conlan MG, et al. 1988. Blood 72:185-190.
3. Sun WH, Mosher DF. 1989. Blood 73:353-354.
4. Sigurdardottir O, Wiman B. 1994. Biochim. Biophys. Acta. 1208:104-110.
5. Yaoi Y, et al. 1991. Exp. Cell Res. 194: 180-185.
6. Seiffert D, Loskutoff DJ. 1991. J. Biol. Chem. 266:2824-2830.
7. Lim BL, et al. 1996. J. Biol. Chem. 271:26739-26744.
8. Zhou A, et al. 2003. Nat. Struct. Biol. 10:541-544.
9. Ekmekci OB, et al. 2006. Clin. Chim. Acta. 368(1-2):77-83.
10. Derer W, et al. 2009. Circ. Cardiovasc. Interv. 2(1):14-9.
11. Heyman L, et al. 2010. Cell Biol. Int. 34(5):493-502.

Gene ID
7448 View all products for this Gene ID
UniProt
View information about Vitronectin on UniProt.org

Related FAQs

Why choose BioLegend recombinant proteins?

     • Each lot of product is quality-tested for bioactivity as indicated on the data sheet.
     • Greater than 95% Purity or higher, tested on every lot of product.
     • 100% Satisfaction Guarantee for quality performance, stability, and consistency.
     • Ready-to-use liquid format saves time and reduces challenges associated with reconstitution.
     • Bulk and customization available. Contact us.
     • Learn more about our Recombinant Proteins.

How does the activity of your recombinant proteins compare to competitors?

We quality control each and every lot of recombinant protein. Not only do we check its bioactivity, but we also compare it against other commercially available recombinant proteins. We make sure each recombinant protein’s activity is at least as good as or better than the competition’s. In order to provide you with the best possible product, we ensure that our testing process is rigorous and thorough. If you’re curious and eager to make the switch to BioLegend recombinants, contact your sales representative today!

What is the specific activity or ED50 of my recombinant protein?

The specific activity range of the protein is indicated on the product datasheets. Because the exact activity values on a per unit basis can largely fluctuate depending on a number of factors, including the nature of the assay, cell density, age of cells/passage number, culture media used, and end user technique, the specific activity is best defined as a range and we guarantee the specific activity of all our lots will be within the range indicated on the datasheet. Please note this only applies to recombinants labeled for use in bioassays. ELISA standard recombinant proteins are not recommended for bioassay usage as they are not tested for these applications.

Have your recombinants been tested for stability?

Our testing shows that the recombinant proteins are able to withstand room temperature for a week without losing activity. In addition the recombinant proteins were also found to withstand four cycles of freeze and thaw without losing activity.

Does specific activity of a recombinant protein vary between lots?

Specific activity will vary for each lot and for the type of experiment that is done to validate it, but all passed lots will have activity within the established ED50 range for the product and we guarantee that our products will have lot-to-lot consistency. Please conduct an experiment-specific validation to find the optimal ED50 for your system.

How do you convert activity as an ED50 in ng/ml to a specific activity in Units/mg?

Use formula Specific activity (Units/mg) = 10^6/ ED50 (ng/mL)

Go To Top Version: 1    Revision Date: 06/28/2016

For Research Use Only. Not for diagnostic or therapeutic use.

 

This product is supplied subject to the terms and conditions, including the limited license, located at www.biolegend.com/terms) ("Terms") and may be used only as provided in the Terms. Without limiting the foregoing, BioLegend products may not be used for any Commercial Purpose as defined in the Terms, resold in any form, used in manufacturing, or reverse engineered, sequenced, or otherwise studied or used to learn its design or composition without express written approval of BioLegend. Regardless of the information given in this document, user is solely responsible for determining any license requirements necessary for user’s intended use and assumes all risk and liability arising from use of the product. BioLegend is not responsible for patent infringement or any other risks or liabilities whatsoever resulting from the use of its products.

 

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