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- Regulatory Status
- RUO
- Other Names
- GMA, S-MAG, SPG75, SIGLEC4A, SIGLEC-4A, Myelin Associated Glycoprotein, Sialic Acid Binding Ig-Like Lectin 4A
- Ave. Rating
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- Product Citations
- publications
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Recombinant Human Siglec-4a-Fc chimera binds to immobilized recombinant human MUC-1 (Cat. No. 797304) at 5 ug/mL. The ED50 for this effect is 1.5 – 7.5 µg/mL. -
Stability Testing for Recombinant Human Siglec-4a-Fc Chimera. Recombinant Human Siglec-4a-Fc Chimera was aliquoted in PBS at 0.2 mg/mL and one aliquot was kept at 4°C (Control), and another was frozen and thawed four times (4x Freeze/Thaw). The samples were tested by their ability to bind to immobilized recombinant human MUC-1 (Cat. No. 797304) at 5 µg/mL. The ED50 for this effect is 1.5 – 7.5 µg/mL.
| Cat # | Size | Price | Quantity Check Availability | Save | ||
|---|---|---|---|---|---|---|
| 799104 | 25 µg | 156€ | ||||
| 799106 | 100 µg | 628€ | ||||
Siglec proteins (sialic acid binding Ig-like lectins) are type I membrane proteins with an extracellular region containing a sialic acid binding V-set Ig-like domain at the N-terminus, followed by varying numbers of C2-set Ig domains. In the cytosolic domain, most Siglecs contain a combination of tyrosine motifs, including immunoreceptor tyrosine-based inhibitory motifs (ITIM), along with ITIM-like, Grb2-binding, and Fyn kinase sites. Siglecs are widely expressed on hematopoietic cells, often in a cell-type-specific manner. Their ligands, sialic acids, are negatively charged monosaccharides found on cell-surface glycoproteins and glycolipids. Siglecs can be classified into two subgroups with Siglec 1, 2, and 4, as one group and a Siglec-3/CD33-related subgroup (Siglec 3 and 5 through 14) as the second. Siglec 1 and Siglec 4 (myelin-associated glycoprotein, MAG) lack the ITIM and ITAM (immune receptor tyrosine-based activation motif) domains. Siglec-4a contains five Ig-like domains (one V-set domain and four C2-set). Siglec 4a is mainly involved in myelination and cell-cell contact. Siglec-4 is mostly expressed in Schwann cells (in the peripheral nervous system) and oligodendrocytes (in the central nervous system). MAG-null mice present neuropathies with loss of axon-myelin connectivity. Siglec 1, 2, 4, and 15 are conserved in vertebrates, and it seems that Siglec 1 and Siglec 4 are ancient receptors. Jawless fishes are primitive form of vertebrates; these do not have myelin and seems to lack Siglec 4.
Product DetailsProduct Details
- Source
- Human Siglec 4a, amino acid Gly20-Pro516 (accession # P20916), with a linker (SR-IEGRMD) and a C-terminal human IgG (Pro100-Lys330) tag, was expressed in 293E cells.
- Molecular Mass
- The 736 amino acid recombinant protein has a predicted molecular mass of approximately 81.56 kD. The DTT-reduced and non-reduced protein migrates at approximately 100 kD and 200 kD respectively by SDS-PAGE. The predicted N-terminal amino acid is Gly.
- Purity
- > 95%, as determined by Coomassie stained SDS-PAGE.
- Formulation
- 0.22 µm filtered protein solution is in PBS.
- Endotoxin Level
- Less than 0.1 EU per µg cytokine as determined by the LAL method.
- Concentration
- 25 µg size is bottled at 200 µg/mL. 100 µg size and larger sizes are lot-specific and bottled at the concentration indicated on the vial. To obtain lot-specific concentration and expiration, please enter the lot number in our Certificate of Analysis online tool.
- Storage & Handling
- Unopened vial can be stored between 2°C and 8°C for up to 2 weeks, at -20°C for up to six months, or at -70°C or colder until the expiration date. For maximum results, quick spin vial prior to opening. The protein can be aliquoted and stored at -20°C or colder. Stock solutions can also be prepared at 50 - 100 µg/mL in appropriate sterile buffer, carrier protein such as 0.2 - 1% BSA or HSA can be added when preparing the stock solution. Aliquots can be stored between 2°C and 8°C for up to one week and stored at -20°C or colder for up to 3 months. Avoid repeated freeze/thaw cycles.
- Activity
- Human Siglec 4a binds to immobilized recombinant human MUC-1 (Cat. No. 797304) at 5 µg/mL. The ED50 for this effect is 1.5 – 7.5 µg/mL.
- Application
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Bioassay
- Application Notes
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BioLegend carrier-free recombinant proteins provided in liquid format are shipped on blue-ice. Our comparison testing data indicates that when handled and stored as recommended, the liquid format has equal or better stability and shelf-life compared to commercially available lyophilized proteins after reconstitution. Our liquid proteins are validated in-house to maintain activity after shipping on blue ice and are backed by our 100% satisfaction guarantee. If you have any concerns, contact us at tech@biolegend.com.
Antigen Details
- Structure
- Disulfide-linked homodimer
- Distribution
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Myelinating cells, Schwann cells, oligodendrocytes
- Function
- Maintains myelin-axon membrane-membrane interactions
- Ligand/Receptor
- Terminal sequence Neu5Acα2-3Galβ1-3GalNAc of gangliosides GD1a and GT1b. Nogo receptors NgR1 and NgR2, CD29, and PIR-B.
- Bioactivity
- Human Siglec-4a binds to immobilized MUC-1
- Cell Type
- Neurons, Oligodendrocytes, Schwann Cells
- Biology Area
- Cell Adhesion, Neurodegeneration, Neuroscience, Neuroscience Cell Markers
- Molecular Family
- Adhesion Molecules
- Antigen References
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- McMillan SJ, et al. 2013. Blood. 121:2084-94.
- Bax M, et al. 2010. Ann Rheum Dis. 69:1191-4.
- Angata T and Varki A. 2000. J Biol Chem. 275:22127-35.
- Yu Z, et al. 2001. Biochem J. 353:483-92.
- Paul SP, et. al. 2000. Blood. 96:483-90.
- Bonhoff KF, et al. 2018. Develop Comp Immunol. 86:219-231.
- Gonzalez-Gil A and Schnaar RL. 2021. Cells. 10:1260.
- Pan B, et al. 2005. Exp Neurol. 195:208-17.
- Gene ID
- 4099 View all products for this Gene ID
- UniProt
- View information about Siglec 4a on UniProt.org
Related FAQs
- Why choose BioLegend recombinant proteins?
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• Each lot of product is quality-tested for bioactivity as indicated on the data sheet.
• Greater than 95% Purity or higher, tested on every lot of product.
• 100% Satisfaction Guarantee for quality performance, stability, and consistency.
• Ready-to-use liquid format saves time and reduces challenges associated with reconstitution.
• Bulk and customization available. Contact us.
• Learn more about our Recombinant Proteins. - How does the activity of your recombinant proteins compare to competitors?
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We quality control each and every lot of recombinant protein. Not only do we check its bioactivity, but we also compare it against other commercially available recombinant proteins. We make sure each recombinant protein’s activity is at least as good as or better than the competition’s. In order to provide you with the best possible product, we ensure that our testing process is rigorous and thorough. If you’re curious and eager to make the switch to BioLegend recombinants, contact your sales representative today!
- What is the specific activity or ED50 of my recombinant protein?
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The specific activity range of the protein is indicated on the product datasheets. Because the exact activity values on a per unit basis can largely fluctuate depending on a number of factors, including the nature of the assay, cell density, age of cells/passage number, culture media used, and end user technique, the specific activity is best defined as a range and we guarantee the specific activity of all our lots will be within the range indicated on the datasheet. Please note this only applies to recombinants labeled for use in bioassays. ELISA standard recombinant proteins are not recommended for bioassay usage as they are not tested for these applications.
- Have your recombinants been tested for stability?
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Our testing shows that the recombinant proteins are able to withstand room temperature for a week without losing activity. In addition the recombinant proteins were also found to withstand four cycles of freeze and thaw without losing activity.
- Does specific activity of a recombinant protein vary between lots?
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Specific activity will vary for each lot and for the type of experiment that is done to validate it, but all passed lots will have activity within the established ED50 range for the product and we guarantee that our products will have lot-to-lot consistency. Please conduct an experiment-specific validation to find the optimal ED50 for your system.
- How do you convert activity as an ED50 in ng/ml to a specific activity in Units/mg?
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Use formula Specific activity (Units/mg) = 10^6/ ED50 (ng/mL)
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