Recombinant Human PLAUR (uPAR) (carrier-free)

Pricing & Availability
Regulatory Status
RUO
Other Names
CD87, MO3, Plasminogen activator urokinase receptor, uPAR, URKR
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Product Citations
publications
uPAUR_Human_RECOM_BA_012715_1
Biotinylated human PLAUR is able to bind immobilized uPA (2 µg/mL) in a dose dependent manner with a ED50 of < 50 ng/mL (black circle). This binding (120 ng/mL of hPLAUR-biotin) can be blocked by non-biotinylated hPLAUR in a dose dependent manner with an IC50 of 150 - 600 ng/mL (open circle).
  • uPAUR_Human_RECOM_BA_012715_1
    Biotinylated human PLAUR is able to bind immobilized uPA (2 µg/mL) in a dose dependent manner with a ED50 of < 50 ng/mL (black circle). This binding (120 ng/mL of hPLAUR-biotin) can be blocked by non-biotinylated hPLAUR in a dose dependent manner with an IC50 of 150 - 600 ng/mL (open circle).
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559702 10 µg 84,00€
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559704 25 µg 132,00€
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559706 100 µg 260,00€
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Cat. No. 559704 is scheduled for discontinuation. Please use the availability checker to determine current availability. The 10 µg and 100 µg sizes are available as an alternative.
Description

PLAUR (plasminogen activator urokinase receptor), also known as urokinase plasminogen activator-receptor (uPAR), is part of the plasminogen activation system, which is involved in tissue reorganization, cell migration, cell cycle regulation, and cell adhesion. PLAUR was originally identified as a saturable binding site for urokinase on the cell surface, and thus, restricting plasminogen activation to the immediate vicinity of the cell membrane. Due to lack of transmembrane or cytoplasmic domains, PLAUR may be anchored to the plasma membrane by a glycosyl-phosphatidylinositol (GPI) moiety following cleavage of the nascent polypeptide near its C-terminus. A soluble form of PLAUR has been identified. The membrane-bound PLAUR is expressed on granulocytes, monocytes/macrophages, dendritic cells, fibroblasts, endothelial cells, and keratinocytes. This molecule plays a role in cell adhesion and chemotaxis through binding to β1, β2, and β3 integrins. Recently, it was found that soluble PLAUR is increased in plasma of patients with active lupus nephritis, suggesting an association with pathological features of the disease. In addition, soluble PLAUR has been linked as a causative factor and also a biomarker of several diseases including tumor metastasis, focal and segmental glomerulosclerosis, hepatocellular carcinoma, and coronary artery disease.

Product Details
Technical Data Sheet (pdf)

Product Details

Source
Human PLAUR, amino acids (Leu23-Arg303) (Accession# NP_002650), was expressed with a C-terminal His tag in the 293E cell line.
Molecular Mass
The 287 amino acid recombinant protein has a predicted molecular mass of approximately 32.1 kD. The protein migrates at 50 kD in non-reducing and DTT-reducing conditions by SDS-PAGE. The predicted N-terminal amino acid is Leu.
Purity
>95%, as determined by Coomassie stained SDS-PAGE.
Formulation
0.22 µm filtered protein solution is in PBS, pH 7.2.
Endotoxin Level
Less than 1.0 EU per µg of protein as determined by the LAL method.
Concentration
10 and 25 µg sizes are bottled at 200 µg/mL. 100 µg size and larger sizes are lot-specific and bottled at the concentration indicated on the vial (please contact technical support for concentration, or use our Lookup tool if you have a lot number.)
Please note, new lots of the 100 µg size will be lot-specific and may differ from previous lots that had a fixed concentration.
Storage & Handling
Unopened vial can be stored between 2°C and 8°C for up to 2 weeks, at -20°C for up to six months, or at -70°C or colder until the expiration date. For maximum results, quick spin vial prior to opening. The protein can be aliquoted and stored at -20°C or colder. Stock solutions can also be prepared at 50 - 100 µg/mL in appropriate sterile buffer, carrier protein such as 0.2 - 1% BSA or HSA can be added when preparing the stock solution. Aliquots can be stored between 2°C and 8°C for up to one week and stored at -20°C or colder for up to 3 months. Avoid repeated freeze/thaw cycles.
Activity
Biotinylated human PLAUR is able to bind immobilized uPA (2 µg/mL) in a dose dependent manner with a ED50 of < 50 ng/mL. This binding (120 ng/mL of hPLAUR-biotin) can be blocked by non-biotinylated hPLAUR in a dose dependent manner with an IC50 of 150 - 600 ng/mL.
Application

Bioassay

Application Notes

uPA at 2 µg/mL in PBS was immobilized on an ELISA plate and used in the binding assay.


BioLegend carrier-free recombinant proteins provided in liquid format are shipped on blue-ice. Our comparison testing data indicates that when handled and stored as recommended, the liquid format has equal or better stability and shelf-life compared to commercially available lyophilized proteins after reconstitution. Our liquid proteins are verified in-house to maintain activity after shipping on blue ice and are backed by our 100% satisfaction guarantee. If you have any concerns, contact us at tech@biolegend.com.

Antigen Details

Structure
Monomer.
Distribution

Granulocytes, monocytes/macrophages, dendritic cells, fibroblasts, endothelial cells, keratinocytes, and activated T cells.

Function
Convert plasminogen to plasmin, cell adhesion, and chemotaxis.
Ligand/Receptor
uPA; LRP1 (CD91)
Bioactivity
Human PLAUR is able to bind uPA in a dose dependent manner.
Biology Area
Cell Adhesion, Cell Cycle/DNA Replication
Molecular Family
Soluble Receptors
Antigen References

1. Llinas P, et al. 2005. EMBO J. 24:1655.
2. Beaufort N, et al. 2004. J. Immunol. 172:540.
3. Tarui T, et al. 2001. J. Biol. Chem. 276:3983.
4. Qin DD, et al. 2014. Lupus 0961203314558857.
5. Thuno M, et al. 2009. Dis. Markers 27:157.
6. Alfano D, et al. 2009. Front Biosci. 14:3782.
7. Spinale JM, et al. 2014. Kidney Int. 10:1038.
8. Chounta A, et al. 2014. Liver Int. 2:601.
9. Harskamp RE and Roe MT. 2014. J. Am. Heart Assoc. 5:e001431.

Gene ID
5329 View all products for this Gene ID
UniProt
View information about PLAUR on UniProt.org

Related FAQs

Why choose BioLegend recombinant proteins?

     • Each lot of product is quality-tested for bioactivity as indicated on the data sheet.
     • Greater than 95% Purity or higher, tested on every lot of product.
     • 100% Satisfaction Guarantee for quality performance, stability, and consistency.
     • Ready-to-use liquid format saves time and reduces challenges associated with reconstitution.
     • Bulk and customization available. Contact us.
     • Learn more about our Recombinant Proteins.

How does the activity of your recombinant proteins compare to competitors?

We quality control each and every lot of recombinant protein. Not only do we check its bioactivity, but we also compare it against other commercially available recombinant proteins. We make sure each recombinant protein’s activity is at least as good as or better than the competition’s. In order to provide you with the best possible product, we ensure that our testing process is rigorous and thorough. If you’re curious and eager to make the switch to BioLegend recombinants, contact your sales representative today!

What is the specific activity or ED50 of my recombinant protein?

The specific activity range of the protein is indicated on the product datasheets. Because the exact activity values on a per unit basis can largely fluctuate depending on a number of factors, including the nature of the assay, cell density, age of cells/passage number, culture media used, and end user technique, the specific activity is best defined as a range and we guarantee the specific activity of all our lots will be within the range indicated on the datasheet. Please note this only applies to recombinants labeled for use in bioassays. ELISA standard recombinant proteins are not recommended for bioassay usage as they are not tested for these applications.

Have your recombinants been tested for stability?

Our testing shows that the recombinant proteins are able to withstand room temperature for a week without losing activity. In addition the recombinant proteins were also found to withstand four cycles of freeze and thaw without losing activity.

Does specific activity of a recombinant protein vary between lots?

Specific activity will vary for each lot and for the type of experiment that is done to validate it, but all passed lots will have activity within the established ED50 range for the product and we guarantee that our products will have lot-to-lot consistency. Please conduct an experiment-specific validation to find the optimal ED50 for your system.

How do you convert activity as an ED50 in ng/ml to a specific activity in Units/mg?
Use formula Specific activity (Units/mg) = 10e6/ ED50 (ng/mL)
Go To Top Version: 1    Revision Date: 01/27/2015

For research use only. Not for diagnostic use. Not for resale. BioLegend will not be held responsible for patent infringement or other violations that may occur with the use of our products.

 

*These products may be covered by one or more Limited Use Label Licenses (see the BioLegend Catalog or our website, www.biolegend.com/ordering#license). BioLegend products may not be transferred to third parties, resold, modified for resale, or used to manufacture commercial products, reverse engineer functionally similar materials, or to provide a service to third parties without written approval of BioLegend. By use of these products you accept the terms and conditions of all applicable Limited Use Label Licenses. Unless otherwise indicated, these products are for research use only and are not intended for human or animal diagnostic, therapeutic or commercial use.

 

BioLegend Inc., 8999 BioLegend Way, San Diego, CA 92121 www.biolegend.com
Toll-Free Phone: 1-877-Bio-Legend (246-5343) Phone: (858) 768-5800 Fax: (877) 455-9587

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