- Regulatory Status
- Other Names
- Delta-like protein 1, H-Delta-1, drosophila Delta homolog 1
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- Product Citations
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DLL1 is a member of the Delta/Serrate/Lag2 (DSL) family of ligands that bind the Notch receptor. Five DSL ligands have been identified in mammals including three Delta-like proteins (Dll1, 3, and 4) and two Serrate-like (Jagged1 and Jagged2). The canonical DSL (Delta, Serrate, Lag2) ligands are type I cell surface proteins, and like Notch have tandem EGF repeats in their extracellular domain. The DSL domain, the amino terminal, and the first two EGF domains are necessary for the binding to the Notch receptor. Jagged1 and Jagged2 have an additional cysteine rich region, not present in the Delta-like proteins. Through a cell-cell interaction the Notch signaling is activated (trans-interaction); the interaction of the ligands with Notch within the same cell (cis-interaction) induces inhibitory cell signaling. The Notch signaling pathway plays important roles in specifying cell fate during embryonic development and contributes to adult homeostasis; thus, Notch plays a key role in the development of embryonic hematopoietic stem cells, modulates T cell-mediated immune responses and development of marginal zone (MZ) B cells. DLL1 enhances the in vitro conversion of human memory CD4 T cells into FOXP3-expressing regulatory T cells. DSL ligands are cleaved by ADAMs (ADAM9, 10, 12, and 17) resulting in the shedding of the extracellular domain. Soluble intracellular derivatives are generated by presenilin-dependent intramembranous gamma-secretase processing. DLL1 intracellular domain (DICD) generated by the gamma-cleavage is transported into the nucleus where it plays a role in transcriptional events.Product Details
- Human DLL1, amino acids (Ser22- Phe543 ) (Accession# NM_005618) was expressed in E.coli.
- Molecular Mass
- The 522 amino acid recombinant protein has a predicted molecular mass of approximately 56.2kD. The predicted N-terminal amino acid is Ser.
- >95%, as determined by Coomassie stained SDS-PAGE.
- Lyophilized from 0.2 µm filtered protein solution from PBS, pH 7.5 with no additives.
- Endotoxin Level
- Less than 1 EU per µg cytokine as determined by the LAL method.
- Storage & Handling
- Unopened vial can be stored at -20°C or -70°C. For maximum results, quick spin vial prior to opening. Reconstitute in water to a concentration of 0.1-1.0 mg/ml. Do not vortex. It is recommended to further dilute in a buffer containing a carrier protein such as 0.1% BSA and store working aliquots at -20°C to -80°C. Avoid repeated freeze/thaw cycles.
- Immobilized human DLL1 enhances BMP-2 induction of alkaline phosphatase in mouse chondrogenic ATDC5 cells in a dose dependent manner. The ED50 = 0.15 - 0.75 µg/ml.
Thymic stromal cells, fibroblasts in secondary lymphoid organs niches.
- DLL1-mediated cell—cell interactions, play an important role in somitogenesis, myogenesis, retinal development , homeostasis of intestinal stem cells, and differentiation of the intestinal epithelium.
- Multiple stem cells precursors, bone marrow precursors, memory CD4 T cells, peripheral T cells, paraxial (somitic) mesoderma.
- Notch receptors.
- Immobilized human DLL1 enhances BMP-2 induction of alkaline phosphatase in mouse chondrogenic ATDC5 cells.
- Cell Type
- Embryonic Stem Cells
- Biology Area
- Angiogenesis, Apoptosis/Tumor Suppressors/Cell Death, Cell Biology, Immunology, Stem Cells
- Molecular Family
- Adhesion Molecules
- Antigen References
1. Ikeuchi T, et al. 2003. J. Biol. Chem. 278:7751.
2. Parks AL, et al. 2006. Genetics 174:1947.
3. Schuster-Gossler K, et al. 2007. Proc. Natl. Acad. Sci. USA 104:537.
4. Sun D, et al. 2008. J. Cell. Sci. 121:3815.
5. Rocha SF, et al. 2009. Dev. Biol. 328:54.
6. D'Souza, et al. 2010. Curr. Top. Dev. Biol. 92:73.
7. Radtke F, et al. 2010. Immunity 32:14.
8. Pellegrinet L, et al. 2011. Gastroenterology 140:1230.
9. Zhang L, et al. 2011. Biochim. Biophys. Acta. 1813:2036.
10. Mota C, et al. 2014. J. Immunol. 193:5854.
11. Fasnacht N, et al. 2014. J. Exp. Med. 211:2265.
12. Shimojo H, et al. 2016. Genes Dev. 30:102.
- Gene ID
- 28514 View all products for this Gene ID
- View information about DLL1 on UniProt.org
- Why choose BioLegend recombinant proteins?
• Each lot of product is quality-tested for bioactivity as indicated on the data sheet.
• Greater than 95% Purity or higher, tested on every lot of product.
• 100% Satisfaction Guarantee for quality performance, stability, and consistency.
• Ready-to-use liquid format saves time and reduces challenges associated with reconstitution.
• Bulk and customization available. Contact us.
• Learn more about our Recombinant Proteins.
- How does the activity of your recombinant proteins compare to competitors?
We quality control each and every lot of recombinant protein. Not only do we check its bioactivity, but we also compare it against other commercially available recombinant proteins. We make sure each recombinant protein’s activity is at least as good as or better than the competition’s. In order to provide you with the best possible product, we ensure that our testing process is rigorous and thorough. If you’re curious and eager to make the switch to BioLegend recombinants, contact your sales representative today!
- What is the specific activity or ED50 of my recombinant protein?
The specific activity range of the protein is indicated on the product datasheets. Because the exact activity values on a per unit basis can largely fluctuate depending on a number of factors, including the nature of the assay, cell density, age of cells/passage number, culture media used, and end user technique, the specific activity is best defined as a range and we guarantee the specific activity of all our lots will be within the range indicated on the datasheet. Please note this only applies to recombinants labeled for use in bioassays. ELISA standard recombinant proteins are not recommended for bioassay usage as they are not tested for these applications.
- Have your recombinants been tested for stability?
Our testing shows that the recombinant proteins are able to withstand room temperature for a week without losing activity. In addition the recombinant proteins were also found to withstand four cycles of freeze and thaw without losing activity.
- Does specific activity of a recombinant protein vary between lots?
Specific activity will vary for each lot and for the type of experiment that is done to validate it, but all passed lots will have activity within the established ED50 range for the product and we guarantee that our products will have lot-to-lot consistency. Please conduct an experiment-specific validation to find the optimal ED50 for your system.
- How do you convert activity as an ED50 in ng/ml to a specific activity in Units/mg?
Use formula Specific activity (Units/mg) = 10^6/ ED50 (ng/mL)