- T2-1SC (See other available formats)
- Regulatory Status
- Other Names
- TNF receptor-associated factor 6 (TRAF6), E3 ubiquitin protein ligase, Interleukin-1 signal transducer
- Mouse IgG1, κ
- Ave. Rating
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- Product Citations
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The protein encoded by this gene is a member of the TNF receptor-associated factor (TRAF) protein family. TRAFs (TNF receptor-associated factors) are a family of multifunctional adaptor proteins that bind to surface receptors and recruit additional proteins to form multiprotein signaling complexes capable of promoting cellular responses. Members of the TRAF family share a common carboxy-terminal "TRAF domain" which mediates interactions with associated proteins; many also contain amino-terminal Zinc/RING finger motifs. The six known TRAFs (TRAF1-6) act as adaptor proteins for a wide range of cell surface receptors and participate in the regulation of cell survival, proliferation, differentiation, and stress responses.
TRAF6 plays a critical role in innate and adaptive immunity, bone metabolism, and development of certain tissues including the nervous system. This protein has an amino terminal RING domain which is followed by four zinc-finger motifs, a central coiled-coil region and a highly conserved carboxyl terminal domain, known as the TRAF-C domain. TRAF6 deficiency results in osteopetrosis and defective IL-1, CD40, and LPS signaling as well as defects in neuronal development. Unlike other TRAF family members that mediate signaling through TNF, TRAF6 has unique binding activities that result in signaling responses from the interleukin-1 receptor (IL-1R), toll-like receptor, CD40, RANK, and p75 neurotrophin receptor. TRAF6 associates directly with CD40 and RANK, and indirectly with IL-1R/TLR through IRAK. This leads to activation of NF-κB and MAP kinase signaling pathways through downstream association with the TAB/TAK-1 complex. TRAF6 also activates Src family nonreceptor tyrosine kinases leading to Akt activation.
- Verified Reactivity
- Human, Mouse
- Antibody Type
- Host Species
- Partial recombinant human TRAF6 protein (352-522 aa) expressed from E. coli.
- This antibody is provided in phosphate-buffered solution, pH 7.2, containing 0.05% sodium azide.
- Affinity purified
- 0.5 mg/ml
- Storage & Handling
- Upon receipt, store undiluted between 2°C and 8°C.
WB - Quality tested
- Recommended Usage
Each lot of this antibody is quality control tested by Western blotting. For Western blotting, the suggested use of this reagent is 0.5 µg per ml. It is recommended that the reagent be titrated for optimal performance for each application.
- Product Citations
AB_2561867 (BioLegend Cat. No. 654501)
AB_2561868 (BioLegend Cat. No. 654502)
- 522 amino acids, predicted MW of 60 kD
- Cytoplasm, Nucleus
- A unique member of the TRAF family of adaptor proteins involved in both the TNF receptor superfamily and the interleukin-1 receptor (IL-1R)/Toll-like receptor (TLR) superfamily signal transduction pathways
- Homotrimer, homooligomer; Binds to TNFRSF5/CD40 and TNFRSF11A/RANK; Interacts with RBCK1 and TRAF3IP2
- Biology Area
- Cell Biology, Neuroinflammation, Neuroscience, Signal Transduction, Ubiquitin/Protein Degradation
- Antigen References
1. Rothe M, et al. 1994. Cell 78:681.
2. Arch RH, et al. 1998. Genes Dev. 12:2821.
3. Ye H, et al. 2002. Nature 418:443.
4. Yang WL, et al. 2009. Science 325:1134.
5. Qu F, et al. 2012. Mol. Cell. Biol. 32:3925.
6. Akiyama T, et al. 2012. Front Immunol. 3:278.
7. Wei J, et al. 2012. PLoS One 1:e49567.
- Gene ID
- 7189 View all products for this Gene ID 22034 View all products for this Gene ID
- View information about TRAF6 on UniProt.org
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