- 1H4B01 (See other available formats)
- Regulatory Status
- Other Names
- TIR domain-containing adapter molecule 1, TRIF, MyD88-3, Proline-Rich, Vinculin And TIR Domain-Containing Protein B (PRVTIRB)
- Mouse IgG2a, κ
- Ave. Rating
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- Product Citations
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TICAM-1, also known as TRIF, is an intracellular adaptor protein utilized by TLR3 and TLR4. TLR3 recognizes double stranded viral RNA and induces TICAM-1 mediated type I interferon production. The N-terminal region of TICAM-1 recruits TBK1 and IKKi, which in turn phosphorylates the interferon regulatory transcription factor IRF3. The phosphorylated IRF3 is subsequently dimerized and translocates to the nucleus, resulting in transcriptional activation of the gene encoding IFN-β. Mice lacking in TICAM-1 expression are defective in the TLR3 mediated induction of proinflamatory cytokines, suggesting that TICAM-1 is crucial for TLR3 downstream signaling. TICAM-1 also mediates TLR4-induced signaling in the presence of the adaptor molecule TRAM. The C-terminal region of TICAM-1 recruits RIP1 and induces apoptosis through FADD/caspase cascade.Product Details
- Human, Mouse
- Antibody Type
- Host Species
- Partial human TICAM-1 recombinant protein (29-204 a.a.)
- Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide.
- The antibody was purified by affinity chromatography.
- 0.5 mg/ml
- Storage & Handling
- The antibody solution should be stored undiluted between 2°C and 8°C.
WB - Quality tested
ICC, IP - Verified
- Recommended Usage
Each lot of this antibody is quality control tested by Western blotting. For Western blotting, the suggested use of this reagent is 0.1 - 1.0 µg per ml. For immunocytochemistry, a concentration of 5.0 μg/ml is recommended. For immunoprecipitation, the suggested use of this reagent is 2.0 - 10 μg per ml. It is recommended that the reagent be titrated for optimal performance for each application.
- Product Citations
AB_2562543 (BioLegend Cat. No. 657102)
- 712 amino acids, predicted molecular weight of 76 kD. Contains a Toll/interleukin-1 receptor (TIR) homology domain responsible for mediating protein-protein interactions.
Cytosol, endosome membrane
- TICAM-1 acts as an adaptor protein of TLR3 and TLR4 (through TRAM), mediating innate immune responses during viral infection.
- TICAM-1 interacts with TLR3, TRAM, TBK1, IRF3, IRF7, TRAF3, TRAF6, and IKKi.
- Biology Area
- Cell Biology, Immunology, Innate Immunity, Neuroscience
- Molecular Family
- Toll Like Receptors
- Antigen References
1. Petnicki-Ocwieja T, et al. 2013. Infect Immun. 81:402.
2. Riad A, et al. 2011. J. Immunol. 186:2561.
3. Choi YJ, et al. 2010. J. Biol. Chem. 285:21382.
4. Gais P, et al. 2010. J. Immunol. 184:5842.
5. Han KJ, et al. 2010. J. Biol. Chem. 285:12543.
6. Takeda K and Akira S. 2005. Int. Immunol. 17:1.
- Gene ID
- 148022 View all products for this Gene ID
- View information about TICAM-1 on UniProt.org
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