Purified anti-T7 Epitope Tag Antibody (Previously Covance catalog# PRB-186P)

Pricing & Availability
Clone
Poly29032 (See other available formats)
Other Names
Major capsid protein 10A, Major capsid protein, MASMTGGQQMG epitope tag, MASMTGGQQMG tag, Minor capsid protein 10B, T7 epitope tag
Previously
Covance Catalog# PRB-186P
Isotype
Rabbit Polyclonal IgG
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Product Citations
publications
Cat # Size Price Quantity Avail. Save
903201 100 µl 225€
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Description

The T7 tag is an 11 amino acid sequence encoded in the leader sequence of T7 bacteriophage gene10. This gene encodes a T7 major capsid protein whose function is not clear. The T7 tag is used in many expression vectors including the pET system. Antibodies specific for T7 tag are used for studying expression of recombinant T7-tagged proteins.

Product Details
Technical Data Sheet (pdf)

Product Details

Antibody Type
Polyclonal
Host Species
Rabbit
Immunogen
Polyclonal T7 antisera was raised against the peptide T7 (MASMTGGQQMG) conjugated to KLH. The antisera was purified via affinity chromatography using the peptide immobilized on solid support.
Formulation
Phosphate-buffered solution containing 0.1% sodium azide.
Preparation
The antibody was purified by affinity chromatography.
Concentration
1.0 mg/ml
Storage & Handling
Store at -20°C. Upon initial thawing, apportion into working aliquots and store at -20°C. Avoid repeated freeze-thaw cycles to prevent denaturing the antibody.
Application

WB, IHC, ICC, ELISA, Purification

Recommended Usage

The optimal working dilution should be determined for each specific assay condition. This antibody is sold for laboratory research use only, not for human or in-vivo use. BioLegend antibodies may not be resold or modified for resale without prior written approval.
WB: 1:1,000-30,000*
IF: 1:200-2,000
ELISA: 1:100-500 (coating), 1:1,000-30,000 (primary)

Application Notes

This antibody is effective in immunoblotting, immunohistochemistry, immunocytochemistry and ELISA.

*Our Posi-Tag Control Protein (931301) can be used as a helpful positive control for this antibody.


This product may contain other non-IgG subtypes.

RRID
AB_2565029 (BioLegend Cat. No. 903201)

Antigen Details

Biology Area
Cell Biology
Gene ID
NA
UniProt
View information about T7 on UniProt.org

Related FAQs

There are no FAQs for this product.
Go To Top Version: 3    Revision Date: 01/25/2019

For research use only. Not for diagnostic use. Not for resale. BioLegend will not be held responsible for patent infringement or other violations that may occur with the use of our products.

 

*These products may be covered by one or more Limited Use Label Licenses (see the BioLegend Catalog or our website, www.biolegend.com/ordering#license). BioLegend products may not be transferred to third parties, resold, modified for resale, or used to manufacture commercial products, reverse engineer functionally similar materials, or to provide a service to third parties without written approval of BioLegend. By use of these products you accept the terms and conditions of all applicable Limited Use Label Licenses. Unless otherwise indicated, these products are for research use only and are not intended for human or animal diagnostic, therapeutic or commercial use.

 

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Toll-Free Phone: 1-877-Bio-Legend (246-5343) Phone: (858) 768-5800 Fax: (877) 455-9587

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