Purified anti-STAT3 Phospho (Tyr705) Antibody

Pricing & Availability
Clone
13A3-1 (See other available formats)
Other Names
Signal transducer and activator of transcription 3, Acute-phase response factor, APRF, HIES
Isotype
Mouse IgG1, κ
Ave. Rating
1 reviews
Product Citations
publications
1_13A3dash1_PURE_STAT3_Tyr705_Antibody_WB_3_123118.png
Total cell lysates (15 µg total protein) from A431 cells serum starved and untreated (-) or stimulated with 100 ng/mL hEGF for 5 minutes were resolved by 4-12% Bis-Tris gel electrophoresis, transferred to a nitrocellulose membrane, and probed with 0.5 µg/mL (1:1000 dilution) of Purified anti-STAT3 Phospho (Tyr705) Antibody, clone 13A3-1, for 2 hours at room temperature. Proteins were visualized by chemiluminescence detection using HRP goat anti-mouse IgG Antibody (Cat. No. 405306) at a 1:3000 dilution. Purified anti-STAT3 pan Antibody, Clone 4G4B45 (Cat. 678002), was used as a pan STAT3 loading control at 1.0 µg/mL (1:500 dilution). Lane M: Molecular Weight marker.
  • 1_13A3dash1_PURE_STAT3_Tyr705_Antibody_WB_3_123118.png
    Total cell lysates (15 µg total protein) from A431 cells serum starved and untreated (-) or stimulated with 100 ng/mL hEGF for 5 minutes were resolved by 4-12% Bis-Tris gel electrophoresis, transferred to a nitrocellulose membrane, and probed with 0.5 µg/mL (1:1000 dilution) of Purified anti-STAT3 Phospho (Tyr705) Antibody, clone 13A3-1, for 2 hours at room temperature. Proteins were visualized by chemiluminescence detection using HRP goat anti-mouse IgG Antibody (Cat. No. 405306) at a 1:3000 dilution. Purified anti-STAT3 pan Antibody, Clone 4G4B45 (Cat. 678002), was used as a pan STAT3 loading control at 1.0 µg/mL (1:500 dilution). Lane M: Molecular Weight marker.
  • 2_13A3dash1_PURE_STAT3_Tyr705_Antibody_WB_040317
    Total cell lysate from PC3 (lane 1, 15 µg), untreated HepG2 (lane 2, 15 µg), HepG2 treated with 10 ng/ml of IL-6 for 15 minutes (lane 3, 15 µg) and HeLa (lane 4, 15 µg) were resolved by electrophoresis (4-12% Bis-Tris), transferred to nitrocellulose, and probed with 1 µg/ml Purified anti-STAT3 Phospho (Tyr705) antibody (clone 13A3-1). Proteins were visualized using an HRP Goat anti-mouse IgG (clone Poly4053) antibody and chemiluminescence detection. Direct-Blot™ HRP anti-β-actin antibody (clone 2F1-1) was used as a loading control.
  • 3_13A3dash1_PURE_STAT3_Tyr705_Antibody_WB_2_051617
    15 µg of total cell lysate from untreated NIH3T3 (lane 1) and NIH3T3 treated with 100 ng/ml of IL-6 for 30 minutes (lane 2) were resolved by electrophoresis (4-20% Tris-Glycine), transferred to nitrocellulose, and probed with 1 µg/ml purified anti-STAT3 Phospho (Tyr705) antibody (clone 13A3-1). Proteins were visualized using an HRP Goat anti-mouse IgG (clone Poly4053) antibody and chemiluminescence detection. Direct-Blot™ HRP anti-β-actin antibody (clone 2F1-1) was used as a loading control.
  • 4_13A3dash1_PURE_STAT3_Tyr705_Antibody_ICC_4_123118.png
    Serum starved A431 cells were untreated (panel A) or stimulated with 100 ng/mL hEGF for 5 minutes (panel B). Cells were fixed with 4% paraformaldehyde for 10 minutes, permeabilized with ice-cold methanol, and blocked with 5% FBS for 60 minutes. Fixed cells were then intracellularly stained with 5 µg/mL (1:100 dilution) of Purified anti-STAT3 Phospho (Tyr705) Antibody, clone 13A3-1 for 2 hours at room temperature, after which proteins were visualized with Alexa Fluor® 594 goat anti-mouse IgG (Cat. No. 405326) at 2.5 µg/mL (1:200 dilution). Nuclei were counterstained with DAPI, and the image was captured with a 60X objective.
Cat # Size Price Quantity Avail. Save
651001 25 µg 100€
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651002 100 µg 220€
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Description

Tyrosine phosphorylation of STAT3 at Tyr705 occurs in response to LIF, IL-6, leptin, OSM, EGF, PDGF, and HGF. It plays a key role in cell growth and apoptosis through mediating expression of a variety of genes in response to the stimuli.

Product Details
Technical Data Sheet (pdf)

Product Details

Reactivity
Human, Mouse
Antibody Type
Monoclonal
Host Species
Mouse
Immunogen
KLH conjugated modified synthetic peptide
Formulation
This antibody is provided in phosphate-buffered solution, pH 7.2, containing 0.05% sodium azide.
Preparation
The antibody was purified by affinity chromatography.
Concentration
0.5 mg/ml
Storage & Handling
Upon receipt, store undiluted between 2°C and 8°C.
Application

WB - Quality tested
ICC - Validated

Recommended Usage

Each lot of this antibody is quality control tested by Western blotting. For Western blotting, the suggested use of this reagent is 0.5 - 1.0 µg per ml (1:500 - 1:1000 dilution). For immunocytochemistry, a concentration of 0.5 μg/ml is recommended. It is recommended that the reagent be titrated for optimal performance for each application.

Application Notes

The STAT3 Phospho (Tyr705) antibody recognizes the regulatory tyrosine phosphorylation of human STAT3 protein and has been shown to be useful for Western blotting.

During PD testing, this clone failed to stain PFA-fixed cells that had been permeabilized with Triton X-100. We highly recommend permeabilizing cells with methanol.

RRID
AB_10897947 (BioLegend Cat. No. 651001)
AB_10900983 (BioLegend Cat. No. 651002)

Antigen Details

Structure
STAT3 is a 770 amino acid protein of 88 kD. It consists of a DNA binding domain, a SH2 domain, a regulatory tyrosine responsible for binding of SH2 domain, and a C-terminal transactivation domain.
Distribution

Ubiquitous.

Function
STAT3 is tyrosine phosphorylated by receptor kinases in response to a variety of cytokines and growth factors. It forms homo- or heterodimer with STAT1 when tyrosine is phosphorylated, and then translocates to nucleus, acting as a transcription regulator. It is also essential for the differentiation of TH17 cells, which is involved in autoimmune diseases.
Cell Sources
Cytoplasm. Translocate to nucleus in response to tyrosine phosphorylation.
Cell Type
Embryonic Stem Cells, Neural Stem Cells
Biology Area
Cell Biology, Neuroscience, Neuroscience Cell Markers, Signal Transduction, Stem Cells, Synaptic Biology, Transcription Factors
Molecular Family
Phospho-Proteins
Antigen References

1. Akira S, et al. 1994. Cell 77:63.
2. Zhang X, et al. 1995. Science 267:1990.
3. Sanchez-Margalet V, et al. 2001. Cell. Immunol. 211:30.
4. Simon A, et al. 2000. Science 290:144.
5. Hoey T, et al. 1999. Adv. Immunol. 71:145.

Regulation
The small GTPase Rac1 binds and regulates activity of STAT3.
Gene ID
6774 View all products for this Gene ID
UniProt
View information about STAT3 Phospho on UniProt.org

Related FAQs

There are no FAQs for this product.
Go To Top Version: 5    Revision Date: 12/31/2018

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