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Purified anti-SFRP4 Antibody

Pricing & Availability
Clone
Poly28584 (See other available formats)
Regulatory Status
RUO
Other Names
SFRP4
Isotype
Rabbit Polyclonal IgG
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Product Citations
publications
Poly28584_Pure_SFRP4_Antibody_1_040519
Western blot of purified anti-SFRP4 antibody (clone Poly28584). Lane 1: Molecular weight marker; Lane 2: 30 µg of HepG2 cell lysate; Lane 3: 40 µg of human heart lysate. The blot was incubated with 2 µg/mL of the primary antibody overnight at 4°C, followed by incubation with HRP-labeled Donkey anti-rabbit IgG (Cat. No. 406401). Enhanced chemiluminescence was used as the detection system.
  • Poly28584_Pure_SFRP4_Antibody_1_040519
    Western blot of purified anti-SFRP4 antibody (clone Poly28584). Lane 1: Molecular weight marker; Lane 2: 30 µg of HepG2 cell lysate; Lane 3: 40 µg of human heart lysate. The blot was incubated with 2 µg/mL of the primary antibody overnight at 4°C, followed by incubation with HRP-labeled Donkey anti-rabbit IgG (Cat. No. 406401). Enhanced chemiluminescence was used as the detection system.
  • Poly28584_Pure_SFRP4_Antibody_2_040519
    IHC staining of purified anti-SFRP4 antibody (clone Poly28584) on formalin-fixed paraffin-embedded human breast cancer tissue. Following antigen retrieval using Sodium Citrate H.I.E.R (Cat. No. 928502), the tissue was incubated with 5 µg/ml of the primary antibody overnight at 4°C. BioLegend’s Ultra Streptavidin (USA) HRP Detection Kit (Multi-Species, DAB, Cat. No. 929901) was used for detection followed by hematoxylin counterstaining, according to the protocol provided. The image was captured with a 40X objective. Scale Bar: 50 µm
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858401 25 µg 100€
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Description

Secreted frizzled-related protein 4 (SFRP4) is a member of the SFRP family that consists of 5 SFRPs that share a common ~120 amino acid long cysteine-rich domain. SFRPs, including SFRP4, have been shown to function as a soluble Wnt signaling modulator, where they compete with Frizzled family receptor to bind to the Wnt ligands. Expression level of SFRP4 is regulated by sex hormones including estrogen and progesterone, and correlates with incidence of breast  and ovarian cancers. IL-1β-induced upregulation of SFRP4 release into the serum from islets years before clinical diagnosis of diabetes can be used as a prognostic tool for type II diabetes.

Product Details
Technical Data Sheet (pdf)

Product Details

Verified Reactivity
Human
Antibody Type
Polyclonal
Host Species
Rabbit
Immunogen
Recombinant SFRP4 (Met265~Val346) with N-terminal His-Tag expressed in E.coli.
Formulation
0.01 M PBS, pH7.4, containing 0.02% NaN3, 50% glycerol.
Preparation
The antibody was purified by affinity chromatography.
Concentration
0.5 mg/ml
Storage & Handling
Store at 4°C for up to 3 months. For longer term storage aliquot into small volumes and store at -20°C.
Application

WB - Quality tested
IHC-P - Verified
Recommended Usage

Each lot of this antibody is quality control tested by Western blotting. For Western blotting, the suggested use of this reagent is 5.0 - 10 µg per ml. For immunohistochemistry on formalin-fixed paraffin-embedded tissue, a concentration range of 5.0 - 10 µg/ml is suggested. It is recommended that the reagent be titrated for optimal performance for each application.

RRID
AB_2801219 (BioLegend Cat. No. 858401)

Antigen Details

Structure
SFRP4 is a 346 amino acid protein with an apparent molecular mass of ~ 40 kD.
Distribution

Tissue Distribution: Endometrium and testis
Cellular Distribution: Extracellular, nucleus, and plasma membrane

Function
Soluble signaling molecule that modulates wnt signaling
Ligand/Receptor
Wnt protein
Biology Area
Cancer Biomarkers, Neuroscience
Antigen References
  1. Pawar NM, et al. 2018. Cell Signal. 45:63
  2. Bergmann K, et al. 2014. Clin Biochem. 47(7-8):529
Gene ID
6424 View all products for this Gene ID
UniProt
View information about SFRP4 on UniProt.org

Related FAQs

There are no FAQs for this product.
Go To Top Version: 1    Revision Date: 04/05/2019

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