Purified anti-p63 (TA) Antibody

Pricing & Availability
Clone
Poly6189 (See other available formats)
Other Names
p63, Isoforms TA p63-α, -β, -γ
Isotype
Rabbit Polyclonal IgG
Ave. Rating
Submit a Review
Product Citations
publications
poly6189_P63_TA_Purified_Antibody_081616
Total cell lysate from 293E cells (lane 1, 15 µg) and 293E cells transfected with human p63 (TA) (lane 2, 15 µg) were resolved by electrophoresis (4-20% Tris-Glycine gel), transferred to nitrocellulose, and probed with purified anti-p63 (TA) antibody (poly6189). Proteins were visualized using an HRP Donkey anti-rabbit IgG Antibody and chemiluminescence detection. Direct-Blot™ HRP anti-β-actin antibody (clone 2F1-1) was used as a loading control.
  • poly6189_P63_TA_Purified_Antibody_081616
    Total cell lysate from 293E cells (lane 1, 15 µg) and 293E cells transfected with human p63 (TA) (lane 2, 15 µg) were resolved by electrophoresis (4-20% Tris-Glycine gel), transferred to nitrocellulose, and probed with purified anti-p63 (TA) antibody (poly6189). Proteins were visualized using an HRP Donkey anti-rabbit IgG Antibody and chemiluminescence detection. Direct-Blot™ HRP anti-β-actin antibody (clone 2F1-1) was used as a loading control.
Cat # Size Price Quantity Avail. Save
618901 50 µl (5 Western blots) 100€
 
Check Availability


Need larger quantities of this item?
Request Bulk Quote

This product is currently unavailable

 
618902 200 µl (20 Western blots) 280€
 
Check Availability


Need larger quantities of this item?
Request Bulk Quote

This product is currently unavailable

 
This product is scheduled for discontinuation. Please use the availability checker to determine current availability.
Description

p63 (TA) is a member of the p53 family. This protein has multiple isoforms TA p63-α, -β, -γ, and δN p63-α, -β, -γ. The molecular weight of the TA p63 isoforms α=77 kD, β=62 kD, and γ=56 kD. This nuclear protein is essential for limb formation, epidermal morphogenesis, epithelial stem cell regeneration; δN isoforms are antagonistic to p53; TA isoforms can transactivate p53 targets. The p63 δN isoforms repress TA isoforms; mutant p53 abrogates p63 transactivation This protein interacts with p53, p73, and the various p63 TA- and δN isoforms. The Poly6189 antibody recognizes human p63 TA isoforms and has been shown to be useful for Western blotting.

Product Details
Technical Data Sheet (pdf)

Product Details

Reactivity
Human, recognizes TA-isoforms
Antibody Type
Polyclonal
Host Species
Rabbit
Immunogen
Modified peptide
Formulation
This antibody is provided in phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide and 50% glycerol.
Preparation
The antibody was purified by antigen-affinity chromatography.
Concentration
Lot-specific (please contact technical support for concentration and total µg amount, or use our Lookup tool if you have a lot number.)
Storage & Handling
Upon receipt, store frozen at -20°C.
Application

WB - Quality tested
ICC, IHC-P - Reported in the literature

Recommended Usage

Each lot of this antibody is quality control tested by Western blotting. Western blotting, suggested working dilution(s): Use 10 µl per 5 ml antibody dilution buffer for each mini-gel. It is recommended that the reagent be titrated for optimal performance for each application.

Application Notes

This product may contain other non-IgG subtypes.

Application References

(PubMed link indicates BioLegend citation)
  1. Ben Khalifa Y, et al. 2011. PLoS Pathog. 7:e1002256. (ICC)
  2. Zhou Y, et al. 2011. Clin. Invest. Med. 34:E184. (IHC-P)
  3. Tordella L, et al. 2013. PNAS. 110:17969. PubMed
  4. Curtis KM, et al. 2015. PLoS One. 10:123642. PubMed
  5. Ahronian LG, et al. 2015. PLoS One.10:123816. PubMed
Product Citations
  1. Khalifa Y, et al. 2011. PLoS One. 6:e1002256. PubMed
  2. Tordella L, et al. 2013. Proc Natl Acad Sci U S A. 110:17969. PubMed
  3. Curtis K, et al. 2015. PLoS One. 10:123642. PubMed
  4. Ahronian L, et al. 2015. PLoS One. 10:123816. PubMed
  5. Ho J, et al. 2016. PLoS One. 11: 0148301. PubMed
  6. Wang C, et al. 2018. Nat Commun. 13:10. PubMed
  7. Karsli Uzunbas G, et al. 2019. J Biol Chem. 10.1074/jbc.RA119.007965. PubMed
RRID
AB_315895 (BioLegend Cat. No. 618901)
AB_2207171 (BioLegend Cat. No. 618902)

Antigen Details

Structure
p53 family; six isoforms, TA p63α, β, γ, approximately 77 kD, 62 kD, 56 kD
Distribution

Nuclear

Function
Essential for limb formation, epidermal morphogenesis, epithelial stem cell regeneration; ΔN isoforms antagonistic to p53; TA isoforms can transactivate p53 targets
Interaction
p53; p73; TA- and ΔN isoforms
Biology Area
Apoptosis/Tumor Suppressors/Cell Death, Cell Biology, Cell Cycle/DNA Replication, Transcription Factors
Antigen References

1. Yang A, et al. 1998. Mol Cell. 2:305.
2. Celli J, et al. 1999. Cell. 99:143.
3. Ghioni P, et al. 2002. Mol Cell Biol. 22:8659.
4. Waltermann A, et al. 2003. Oncogene. 22:5686.

Regulation
ΔN isoforms repress TA isoforms, mutant p53 abrogates p63 transactivation
Gene ID
8626 View all products for this Gene ID
UniProt
View information about p63 on UniProt.org

Related FAQs

There are no FAQs for this product.
Go To Top Version: 5    Revision Date: 08/16/2016

For research use only. Not for diagnostic use. Not for resale. BioLegend will not be held responsible for patent infringement or other violations that may occur with the use of our products.

 

*These products may be covered by one or more Limited Use Label Licenses (see the BioLegend Catalog or our website, www.biolegend.com/ordering#license). BioLegend products may not be transferred to third parties, resold, modified for resale, or used to manufacture commercial products, reverse engineer functionally similar materials, or to provide a service to third parties without written approval of BioLegend. By use of these products you accept the terms and conditions of all applicable Limited Use Label Licenses. Unless otherwise indicated, these products are for research use only and are not intended for human or animal diagnostic, therapeutic or commercial use.

 

BioLegend Inc., 8999 BioLegend Way, San Diego, CA 92121 www.biolegend.com
Toll-Free Phone: 1-877-Bio-Legend (246-5343) Phone: (858) 768-5800 Fax: (877) 455-9587

This data display is provided for general comparisons between formats.
Your actual data may vary due to variations in samples, target cells, instruments and their settings, staining conditions, and other factors.
If you need assistance with selecting the best format contact our expert technical support team.

ProductsHere
Insert Note Here
Save Close Clear
Lab Timer
Tools
Login / Register
Forgot your password? Reset password?
Create an Account