Purified anti-Neurofilament H (NF-H), Nonphosphorylated Antibody (Previously Covance catalog# SMI-32P)

Pricing & Availability
Clone
SMI 32 (See other available formats)
Regulatory Status
RUO
Other Names
Neurofilament heavy polypeptide, NF-H, 200 kD neurofilament protein, neurofilament triplet H protein
Previously
Covance Catalog# SMI-32P
Isotype
Mouse IgG1
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Product Citations
publications
1_SMI-32_PURE_NF-H_Antibody_IHC-P_011218
IHC staining of purified anti-Neurofilament H (NF-H), Nonphosphorylated antibody (clone SMI 32) on formalin-fixed paraffin-embedded mouse brain tissue. Following antigen retrieval using Retrieval-ALL Antigen Unmasking System 3 (Cat. No. 927601), the tissue was incubated with 1 µg/ml of the primary antibody overnight at 4°C. BioLegend’s Ultra Streptavidin (USA) HRP Detection Kit (Multi-Species, DAB, Cat. No. 929901) was used for detection followed by hematoxylin counterstaining, according to the protocol provided. The image was captured with a 40X objective. Scale bar: 50µm
  • 1_SMI-32_PURE_NF-H_Antibody_IHC-P_011218
    IHC staining of purified anti-Neurofilament H (NF-H), Nonphosphorylated antibody (clone SMI 32) on formalin-fixed paraffin-embedded mouse brain tissue. Following antigen retrieval using Retrieval-ALL Antigen Unmasking System 3 (Cat. No. 927601), the tissue was incubated with 1 µg/ml of the primary antibody overnight at 4°C. BioLegend’s Ultra Streptavidin (USA) HRP Detection Kit (Multi-Species, DAB, Cat. No. 929901) was used for detection followed by hematoxylin counterstaining, according to the protocol provided. The image was captured with a 40X objective. Scale bar: 50µm
  • SMI-32_PURE_NeurofilamentH_Antibody_HR_2_090517
    Immunofluorescence staining of anti-Neurofilament H (NF-H), Nonphosphorylated antibody (clone SMI 32) on formalin-fixed paraffin-embedded (FFPE) tissue section from mouse Thalamus. After antigen retrieval using Retrieval-ALL Antigen Unmasking System 3 (Cat. No. 927601), the tissue was blocked with Normal Serum Block for 30 min at room temperature, and then incubated with SMI 32 at 5 µg/mL overnight at 4°C, followed by incubation with Alexa Fluor® 488 Goat anti-mouse IgG (Cat. No. 405319) for one hour at room temperature. The image was captured with a 40X objective (Scale Bar: 20 µm).
  • SMI-32_PURE_NeurofilamentH_Antibody_HR_3_090517
    IHC-Fluorescence staining of Formalin Fixed Paraffin Embedded (FFPE) Rat Cerebellum by anti-Neurofilament H (NF-H), Nonphosphorylated Antibody clone SMI 32. After antigen retrieval using Retrieval-ALL Antigen Unmasking System 3 (Cat. No. 927601), the tissue was blocked with Normal Serum Block for 30 min at room temperature, and then incubated with SMI 32 at 5 µg/mL overnight at 4°C, followed by incubation with Alexa Fluor® 488 Goat anti-mouse IgG (Cat. No. 405319) for one hour at room temperature. The image was captured with a 40X objective (Scale Bar: 20 µm).
  • SMI-32_PURE_NF-H_Antibody_WB_011218
    Western blot of purified anti-Neurofilament H (NF-H), Nonphosphorylated antibody (clone SMI 32). Lane 1: Molecular weight marker; Lane 2: 20 µg of human brain lysate; Lane 3: 20 µg of mouse brain lysate; Lane 4: 20 µg of rat brain lysate. The blot was incubated with 1 ug/mL of the primary antibody overnight at 4°C, followed by incubation with HRP-labeled goat anti-mouse IgG (Cat. No. 405306). Enhanced chemiluminescence was used as the detection system.
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801702 25 µL 90€
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Description

Neurofilaments (NF) are approximately 10 nanometer intermediate filaments found in neurons. They are a major component of the neuronal cytoskeleton, and function primarily to provide structural support for the axon and to regulate the axon diameter. There are three major NF subunits, and the names given to these subunits are based upon the apparent molecular mass of the mammalian subunits on SDS-PAGE. The light or lowest NF (NF-L) runs at 68-70 kD. The medium or middle NF (NF-M) runs at about 145-160 kD, and the heavy or highest NF (NF-H) runs at 200-220 kD. However, the actual molecular weight of these proteins is considerably lower due to the highly charged C-terminal regions of the molecules. The level of NF gene expression correlates with the axonal diameter, which controls how fast electrical signals travel down the axon. Mutant mice with NF abnormalities have phenotypes resembling amyotrophic lateral sclerosis. NF immunostaining is common in diagnostic neuropathology. It is useful for differentiating neurons (positive for NF) from the glia (negative for NF).

Product Details
Technical Data Sheet (pdf)

Product Details

Verified Reactivity
Human, Mouse, Rat
Antibody Type
Monoclonal
Host Species
Mouse
Formulation
Phosphate-buffered solution (no preservatives or carrier proteins).
Preparation
The antibody was purified by affinity chromatography.
Concentration
1 mg/ml
Storage & Handling
The antibody solution should be stored undiluted between 2°C and 8°C. Please note the storage condition for this antibody has been changed from -20°C to between 2°C and 8°C. You can also check your vial or your CoA to find the most accurate storage condition for this antibody.
Application

IHC-P - Quality tested
WB - Verified
Array Tomography, ICC - Reported in the literature, not verified in house

Recommended Usage

Each lot of this antibody is quality control tested by formalin-fixed paraffin-embedded immunohistochemical staining. For immunohistochemistry, a concentration range of 1.0 - 5.0 µg/ml is suggested. For Western blotting, the suggested use of this reagent is 1.0 - 5.0 µg per ml. It is recommended that the reagent be titrated for optimal performance for each application.

Application Notes

Additional reported applications (for the relevant formats) include Western blotting6, immunohistochemistry4,5, immunocytochemistry1,2,3, 7, array tomography8.

Cross-reactivity to monkey tissue has been Reported in the literature, not verified in house4.

This antibody reacts with a nonphosphorylated epitope in neurofilament H of most mammalian species. The reaction is masked when the epitope is phosphorylated. The staining of isolated neurofilament preparations is greatly intensified upon dephosphorylation. Immunocytochemically, SMI 32 visualizes neuronal cell bodies, dendrites, and some thick axons in the central and peripheral nervous systems. However, thin axons are not revealed. Other cells and tissues are unreactive. The antibody distinguishes three subdivisions of the macaque precentral motor cortex. The greater size of the left versus the right superior temporal lobe was found to be due to increased axonal myelination and not due to increased number of glial cells or SMI 32-enumerated neurons, suggesting that the specialization for language in the left temporal lobe is related to increased speed of signal transmission. In cultures of murine cortex, SMI 32 labels a neuronal population with enhanced vulnerability to kainate toxicity most of which are GABAergic and reveal kainate-activated Ca2+ uptake.

Application References

(PubMed link indicates BioLegend citation)
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  4. Redondo J, et al. 2015. Brain Pathol. 25(6):692. (IHC-P) PubMed  
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  7. Theotokis, et al. 2016. J. Neuroinflammation 13(1):265 (IHC-P)
  8. Bennett, et al. 2015. J. Neurosci. Methods 245:25-36 (Array Tomography)
  9. Petzold A, et al. 2011. Brain 134:464. (WB) PubMed  
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RRID
AB_2715852 (BioLegend Cat. No. 801702)
AB_2564642 (BioLegend Cat. No. 801701)

Antigen Details

Structure
Neurofilament H has an apparent molecular mass of 200-220 kD.
Distribution

Tissue distribution: CNS, peripheral nerves and glandular cells of the prostate
Cellular distribution: Cytoskeleton, nucleus, cytosol, and mitochondrion

Function
NF-H Neurofilaments are the major components of the neuronal cytoskeleton. They provide axonal support and regulate axon diameter. Phosphorylation of NF-H results in the formation of interfilament cross bridges that are important in the maintenance of axonal caliber.
Receptors
Phosphorylation seems to play a major role in the functioning of the larger neurofilament polypeptides (NF-M and NF-H), the levels of phosphorylation result in changes to the neurofilament function.
Cell Type
Mature Neurons
Biology Area
Cell Biology, Neuroscience, Neuroscience Cell Markers
Molecular Family
Intermediate Filaments, Phospho-Proteins
Antigen References
  1. Turner M, et al. 2015. Journal of Neuroimmunology. 285: 4. PubMed
  2. Pagliarini V, et al. 2015. J. Cell Biol.. 211: 77. PubMed
  3. Petzold A, et al. 2011. Brain 134. (WB) PubMed 
  4. Yuan A, et al. 2016. Brain Res Bull  126(3): 334.
  5. Parlakian A, et al. 2016. Rev Neurol. 172(10): 607.
  6. Li D, et al. 2016. Front Aging Neurosci. 8: 290.
  7. Costa J, et al. 2016. Clin Chim Acta. 455: 7.
  8. Lad SP, et al. 2010.  J Stroke Cerebrovasc Dis. 21(1): 30.
Gene ID
4744 View all products for this Gene ID
UniProt
View information about Neurofilament H NF-H on UniProt.org

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  • Purified anti-Neurofilament H (NF-H), Nonphosphorylated

  • HRP anti-Neurofilament H (NF-H), Nonphosphorylated

  • Alexa Fluor® 488 anti-Neurofilament H (NF-H), Nonphosphorylated

  • Alexa Fluor® 594 anti-Neurofilament H (NF-H), Nonphosphorylated

  • Alexa Fluor® 647 anti-Neurofilament H, Nonphosphorylated

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