- 6H9B10 (See other available formats)
- Regulatory Status
- Other Names
- FKBP12-rapamycin complex-associated protein 1, FK506 binding protein 12-rapamycin associated protein 1, FRAP1
- Mouse IgG1, κ
- Ave. Rating
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- Product Citations
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mTOR (mammalian target of rapamycin) is a 289 kD member of the PI3 kinase family, containing HEAT, FRB, and FATC domains. mTOR is expressed in the nucleus and cytoplasm, and is a target for cell-cycle arrest and the immunosuppressive effects of FKBP12-rapamycin complex. mTOR controls ribosome biogenesis and cell growth, and acts as an ATP sensor. mTOR is repressed by nutrient deprivation and mitochondrial uncoupling. mTOR is the target of PI3K-regulated protein kinase, AKT/PKB (phosphorylated at Thr 2446 and Ser2448). mTOR has also been shown to bind RAPTOR, which negatively regulates mTOR kinase activity, and is also inhibited by tuberin-hamartin tumor suppressor complex. The binding of RAPTOR to mTOR is necessary for the mTOR-catalyzed phosphorylation of 4EBP1 in vitro, and it strongly enhances the mTOR kinase activity toward p70-alpha.Product Details
- Human, Mouse
- Antibody Type
- Host Species
- Partial human mTOR recombinant protein (1186-1385 aa) expressed in E. coli.
- This antibody is provided in phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide.
- The antibody was purified by affinity chromatography.
- 0.5 mg/ml
- Storage & Handling
- The antibody solution should be stored undiluted between 2°C and 8°C.
WB - Quality tested
- Recommended Usage
Each lot of this antibody is quality control tested by Western blotting. For Western blotting, the suggested use of this reagent is 0.25 - 1.0 µg per ml. It is recommended that the reagent be titrated for optimal performance for each application.
- Product Citations
AB_2563155 (BioLegend Cat. No. 659201)
AB_2563156 (BioLegend Cat. No. 659202)
- 2549 amino acids encode a protein with predicted MW of 289 kD.
- The protein encoded by this gene belongs to a family of phosphatidylinositol kinase-related kinases. These kinases mediate cellular responses to stresses such as DNA damage and nutrient deprivation. This protein acts as the target for cell-cycle arrest and immunosuppressive effects of the FKBP12-rapamycin complex.
- Part of the mammalian target of rapamycin complex 1 (mTORC1) which contains MTOR, MLST8, RPTOR, AKT1S1/PRAS40, and DEPTOR; also part of the mammalian target of rapamycin complex 2 (mTORC2) which contains MTOR, MLST8, PRR5, RICTOR, MAPKAP1, and DEPTOR.
- Biology Area
- Cell Biology, Cell Cycle/DNA Replication, Signal Transduction
- Antigen References
1. Brown EJ, et al. 1994. Nature. 369:756.
2. Dennis P, et al. 2001. Science. 294:1102.
3. Fingar D, et al. 2002. Genes Dev. 16:1472.
4. Nojima H, et al. 2003. J. Biol. Chem. 278:15461.
- Gene ID
- 2475 View all products for this Gene ID
- View information about mTOR on UniProt.org
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