Purified anti-mouse Notch 1 Antibody

Pricing & Availability
Clone
HMN1-12 (See other available formats)
Other Names
Neurogenic locus notch homolog protein 1
Isotype
Armenian Hamster IgG
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HMN1-12_Pure_022309.jpg
Mouse Notch-1 transfected cells stained with purified HMN1-12, followed by anti-Armenian hamster IgG PE
  • HMN1-12_Pure_022309.jpg
    Mouse Notch-1 transfected cells stained with purified HMN1-12, followed by anti-Armenian hamster IgG PE
Cat # Size Price Quantity Avail. Save
130602 500 µg 268€
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Description

The Notch receptors are highly conserved from invertebrates to mammals. Notch 1 and Notch 2 exhibit the greatest structural similarity among the four mammalian Notch receptors. Notch 3 has a number of structural and functional differences. The binding of Notch 3 to its ligands results in the proteolysis of Notch and movement of intracellular portions of Notch into the nucleus. This translocation triggers a series of signaling processes. Notch 3 is primarily expressed in adult arterial smooth muscle cells. Notch 3 gene mutation can cause CADASIL, an inherited early stroke syndrome.

Product Details
Technical Data Sheet (pdf)

Product Details

Reactivity
Mouse
Antibody Type
Monoclonal
Host Species
Armenian Hamster
Immunogen
Notch1-Fc fusion protein
Formulation
Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide.
Preparation
The antibody was purified by affinity chromatography.
Concentration
0.5 mg/ml
Storage & Handling
The antibody solution should be stored undiluted between 2°C and 8°C.
Application

FC - Quality tested
IHC - Reported in the literature

Recommended Usage

Each lot of this antibody is quality control tested by immunofluorescent staining with flow cytometric analysis. For flow cytometric staining, the suggested use of this reagent is ≤0.25 µg per million cells in 100 µl volume. It is recommended that the reagent be titrated for optimal performance for each application.

Application References

(PubMed link indicates BioLegend citation)
  1. Moriyama Y, et al. 2008. Int. Immunol. 20:763.
RRID
AB_1227721 (BioLegend Cat. No. 130602)

Antigen Details

Structure
Transmembrane receptor. Heterodimer consisting of a C-terminal fragment and N-terminal fragment probably linked by disulphide bonds. Contains 5 ankyrin repeats, 29-36 EGF repeats, 3 Notch/Lin repeats.
Distribution

Highly expressed in the brain, lung, and thymus (CD4-CD8- cells and CD4-CD8+ cells). Lower levels of expression in spleen, bone marrow, spinal cord, eyes, mammary gland, liver, intestine, kidney, and heart.

Function
Regulates broad spectrum of various cell lineage commitment processes, including myogenesis, neurogenesis, gliogenesis, and lymphocyte development.
Ligand/Receptor
Jag 1,2; Delta 1,2
Cell Type
Neural Stem Cells
Biology Area
Cell Biology, Immunology, Neuroscience, Neuroscience Cell Markers, Stem Cells, Synaptic Biology
Molecular Family
Postsynaptic proteins
Antigen References

1. Ehebauer ME, et al. 2006. Biochem. J. 392:13.
2. Shimizu K, et al. 2000. Mol. Cell. Biol. 20:18.
3. Tanigaki K, et al. 2007. Nature Immunol. 8:4514.
4. Kraman M, et al. 2005. FASEB J. 19:1311.

Gene ID
18128 View all products for this Gene ID
UniProt
View information about Notch 1 on UniProt.org

Related FAQs

There are no FAQs for this product.
Go To Top Version: 1    Revision Date: 11/30/2012

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