Purified anti-mouse NGAL (Lipocalin-2) Antibody

Pricing & Availability
M047A10 (See other available formats)
Regulatory Status
Other Names
Lipocalin 2, Oncogene 24p3, Neutrophil Gelatinase-Associated Lipocalin, Siderocalin LCN2, 25 kD Alpha-2-Microglobulin-Related Subunit Of MMP-9, p25, MSFI, Migration-Stimulating Factor Inhibitor, HNL
Rat IgG2a, κ
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Product Citations
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532202 500 µg 396,00€
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NGAL (lipocalin-2) is a lipocalin family protein that plays important roles in innate immunity, inflammation, and atherosclerosis. Mouse NGAL can be detected in the lung, spleen, uterus, vagina, and epididymis. It is a critical component of innate immunity due to its ability to bind iron-siderophore complexes and limit bacterial growth. It is also produced by injured epithelia and is one of the most promising new markers for renal epithelial injury and cardiovascular disease (CVD).

Product Details
Technical Data Sheet (pdf)

Product Details

Antibody Type
Host Species
In-house recombinant mouse NGAL
Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide.
The antibody was purified by affinity chromatography.
0.5 mg/ml
Storage & Handling
The antibody solution should be stored undiluted between 2°C and 8°C.

ELISA - Quality tested
IP - Verified

Recommended Usage

Each lot of this antibody is quality control tested by ELISA. For ELISA capture applications, a concentration of 1.5 µg/mL is recommended. To obtain a linear standard curve, serial dilutions of NGAL recombinant protein ranging from 1000 to 15.6 pg/mL is recommended for each ELISA plate. It is recommended that the reagent be titrated for optimal performance.

Application Notes

The purified M047A10 antibody is useful as the capture antibody in a sandwich ELISA assay, when used in conjunction with the biotinylated M0417A8 antibody as the detecting antibody.

For testing mouse NGAL in cell culture supernatant, cell lysate, serum, plasma, urine, and other biological fluids, BioLegend's specially developed LEGEND MAX™ Kits are recommended.

Product Citations
AB_2650711 (BioLegend Cat. No. 532202)

Antigen Details

195 amino acid monomer with a predicted molecular weight of approximately 23 kD.

Secreted by epithelial cells, macrophages, neutrophils; increased levels have been observed in plasma, serum, and urine in various conditions, such as metastatic breast and colorectal cancer, acute kidney injury, pancreatitis and preeclampsia.

Apoptosis, innate immunity, renal development.
Disulfide-linked with MMP9.
Apoptosis, innate immunity, renal development.
Cell Type
Epithelial cells, Macrophages, Neutrophils
Biology Area
Apoptosis/Tumor Suppressors/Cell Death, Cell Biology, Immunology, Innate Immunity, Signal Transduction
Antigen References

1. Iqbal N, et al. 2013. Expert Opin.Med. Diagn. 7:209.
2. Singer E, et al. 2013. Acta. Physiol. (Oxf) 207:663.
3. Palazzuoli A, et al. 2012. Clin. Chim. Acta. 413:1832.
4. Flo TH, et al. 2004. Nature 432:917.
5. Goetz DH, et al. 2002. Mol. Cell. 10:1033.
6. Leung L, et al. 2012. PLoS One 7:e46677.
7. Kjeldsen L, et al. 1993. J. Biol. Chem. 268:10425.

Gene ID
16819 View all products for this Gene ID
View information about NGAL on UniProt.org

Related FAQs

There are no FAQs for this product.
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*These products may be covered by one or more Limited Use Label Licenses (see the BioLegend Catalog or our website, www.biolegend.com/ordering#license). BioLegend products may not be transferred to third parties, resold, modified for resale, or used to manufacture commercial products, reverse engineer functionally similar materials, or to provide a service to third parties without written approval of BioLegend. By use of these products you accept the terms and conditions of all applicable Limited Use Label Licenses. Unless otherwise indicated, these products are for research use only and are not intended for human or animal diagnostic, therapeutic or commercial use.


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