Purified anti-mouse Ly-6G Maxpar Ready Antibody anti-Ly-6G

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Clone: 1A8 (See other available formats)
Regulatory Status: RUO
Other Names: Lymphocyte antigen 6 complex, locus G
Isotype: Rat IgG2a, κ
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Product Citations: publications

1A8_PURE_Ly-6G_Maxpar_Antibody_1_FC_051316 — C57BL/6 mouse bone marrow cells stained with 148Nd-anti-CD11b (M1/70) and 141Pr-anti-Ly-6G (1A8). Data provided by DVS Sciences.

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127637

100 µg

100€

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Description

Lymphocyte antigen 6 complex, locus G (Ly-6G), a 21-25 kD GPI-anchored protein, is expressed on the majority of myeloid cells in bone marrow and peripheral granulocytes.

Product Details

Technical Data Sheet (pdf)

Product Details

Verified Reactivity

Mouse

Antibody Type

Monoclonal

Host Species

Rat

Immunogen

Ly-6G transfected EL-4J cell line.

Formulation

Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide and EDTA.

Preparation

The antibody was purified by affinity chromatography.

Concentration

1.0 mg/ml

Storage & Handling

The antibody solution should be stored undiluted between 2°C and 8°C.

Application

FC - Quality tested
CyTOF®, WB - Verified

Recommended Usage

This product is suitable for use with the Maxpar® Metal Labeling Kits. For metal labeling using Maxpar® Ready antibodies, proceed directly to the step to Partially Reduce the Antibody by adding 100 µl of Maxpar® Ready antibody to 100 µl of 4 mM TCEP-R in a 50 kDa filter and continue with the protocol. Always refer to the latest version of Maxpar® User Guide when conjugating Maxpar® Ready antibodies.

Application Notes

While 1A8 recognizes only Ly-6G, clone RB6-8C5 recognizes both Ly-6G and Ly-6C. Clone RB6-8C5 binds with high affinity to mouse Ly-6G molecules and to a lower extent to Ly-6C¹⁵. Clone RB6-8C5 impairs the binding of anti-mouse Ly-6G clone 1A8¹⁵. However, clone RB6-8C5 is able to stain in the presence of anti-mouse Ly-6C clone HK1.4¹⁶.

Additional reported applications (for the relevant formats) include: immunohistochemistry⁹ of frozen sections¹⁰ and paraffin-embedded sections¹¹, depletion^{4, 12-14}, and spatial biology (IBEX)^20,21. The Ultra-LEAF™ purified antibody (Endotoxin < 0.01 EU/µg, Azide-Free, 0.2 µm filtered) is recommended for in vivo studies or highly sensitive assays (Cat. No. 127632, 127649, 127650, 127661 and 127662).

Additional Product Notes

Maxpar® is a registered trademark of Standard BioTools Inc.

Application References (PubMed link indicates BioLegend citation)

Fleming TJ, et al. 1993. J. Immunol. 151:2399. (FC)
Daley JM, et al. 2008. J. Leukocyte Biol. 83:1. (FC)
Dietlin TA, et al. 2007. J. Leukocyte Biol. 81:1205. (FC)
Daley J, et al. 2007. J. Leukocyte Biol. doi:10.1189. (Deplete) PubMed
Tadagavadi RK, et al. 2010. J. Immunol. 185:4904. PubMed
Sumagin R, et al. 2010. J. Immunol. 185:7057. PubMed
Guiducci C, et al. 2010. J. Exp Med. 207:2931. PubMed
Fujita M, et al. 2011. Cancer Res. 71:2664. PubMed
Van Leeuwen, et al. 2008. Arterioscler. Thromb. Vasc. Biol. 28:84. (IHC)
Kowanetz M, et al. 2010. P. Natl. Acad. Sci. USA 107:21248. [supplementary data] (IHC)
Esbona K, et al. 2016. Breast Cancer Res. 18:35. (IHC)
Wojtasiak M, et al. 2010. J. Gen. Virol. 91:2158. (FC, Deplete)
Jaeger BN, et al. 2012. J. Exp. Med. 209:565. (Deplete)
Wozniak KL, et al. 2012. BMC Immunol. 13:65 (FC, Deplete)
Ribechini E, et al. 2009. Eur. J. Immunol. 39:3538.
Ng LG, et al. 2011. J Invest. Dermatol. 131:2058. PubMed
Ma C, et al. 2012. J. Leukoc. Biol. 92:1199.
McCartney-Francis, N, et al. 2014. J Leukoc. Biol. 96:917. PubMed
Her Z, et al. 2014. EMBO Mol. Med. 7:24. PubMed
Radtke AJ, et al. 2020. Proc Natl Acad Sci U S A. 117:33455-65. (SB) PubMed
Radtke AJ, et al. 2022. Nat Protoc. 17:378-401. (SB) PubMed

Product Citations

Dasgupta D, et al. 2020. Gastroenterology. 159:1487. PubMed
Mariani SA, et al. 2019. Immunity. 50:1439. PubMed
Jordan S, et al. 2020. Cell. 178(5):1102-1114.e17.. PubMed
Rustenhoven J, et al. 2021. Cell. 184(4):1000-1016.e27. PubMed
Zhu YP et al. 2018. Cell reports. 24(9):2329-2341 . PubMed

RRID

AB_2563784 (BioLegend Cat. No. 127637)

Antigen Details

Structure: A 21-35 kD GPI-anchorded membrane protein
Distribution: Expressed on the majority of myeloid cells in bone marrow and peripheral granulocytes. The monoclonal antibody RB6-8C5 recognizes both Ly-6G and Ly-6C.
Cell Type: Granulocytes, Macrophages, Monocytes
Biology Area: Immunology, Innate Immunity
Antigen References: Fleming TJ, et al. 1993. J. Immunol. 151:2399.
Gene ID: 546644 View all products for this Gene ID
UniProt: View information about Ly-6G on UniProt.org

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Related FAQs

Can I obtain CyTOF data related to your Maxpar® Ready antibody clones?

We do not test our antibodies by mass cytometry or on a CyTOF machine in-house. The data displayed on our website is provided by Fluidigm^®. Please contact Fluidigm^® directly for additional data and further details.

http://techsupport.fluidigm.com/

Can I use Maxpar® Ready format clones for flow cytometry staining?

We have not tested the Maxpar^® Ready antibodies formulated in solution containing EDTA for flow cytometry staining. While it is likely that this will work in majority of the situations, it is best to use the non-EDTA formulated version of the same clone for flow cytometry testing. The presence of EDTA in some situations might negatively affect staining.

I am having difficulty observing a signal after conjugating a metal tag to your Maxpar® antibody. Please help troubleshoot.

We only supply the antibody and not test that in house. Please contact Fluidigm^® directly for troubleshooting advice: http://techsupport.fluidigm.com/

Is there a difference between buffer formulations related to Maxpar® Ready and purified format antibodies?

The Maxpar^® Ready format antibody clones are formulated in Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide and EDTA. The regular purified format clones are formulated in solution that does not contain any EDTA. Both formulations are however without any extra carrier proteins.

Other Formats

View All Ly-6G Reagents Request Custom Conjugation

Description	Clone	Applications
Alexa Fluor® 594 anti-mouse Ly-6G	1A8	IHC-F,SB
Purified anti-mouse Ly-6G	1A8	FC,IHC-F,IHC-P,SB
Biotin anti-mouse Ly-6G	1A8	FC,IHC
FITC anti-mouse Ly-6G	1A8	FC
PE anti-mouse Ly-6G	1A8	FC
Alexa Fluor® 647 anti-mouse Ly-6G	1A8	FC,IHC-F,SB
Pacific Blue™ anti-mouse Ly-6G	1A8	FC
APC anti-mouse Ly-6G	1A8	FC
PerCP/Cyanine5.5 anti-mouse Ly-6G	1A8	FC
PE/Cyanine7 anti-mouse Ly-6G	1A8	FC
Alexa Fluor® 700 anti-mouse Ly-6G	1A8	FC,IHC
APC/Cyanine7 anti-mouse Ly-6G	1A8	FC
Alexa Fluor® 488 anti-mouse Ly-6G	1A8	FC,IHC-F,SB
Brilliant Violet 421™ anti-mouse Ly-6G	1A8	FC,IHC-F
Brilliant Violet 570™ anti-mouse Ly-6G	1A8	FC
Ultra-LEAF™ Purified anti-mouse Ly-6G	1A8	FC,Depletion,IHC
Brilliant Violet 510™ anti-mouse Ly-6G	1A8	FC
Purified anti-mouse Ly-6G (Maxpar® Ready)	1A8	FC,CyTOF®,WB
Brilliant Violet 650™ anti-mouse Ly-6G	1A8	FC
Brilliant Violet 711™ anti-mouse Ly-6G	1A8	FC
Brilliant Violet 605™ anti-mouse Ly-6G	1A8	FC
Brilliant Violet 785™ anti-mouse Ly-6G	1A8	FC
PE/Dazzle™ 594 anti-mouse Ly-6G	1A8	FC
APC/Fire™ 750 anti-mouse Ly-6G	1A8	FC
PerCP anti-mouse Ly-6G	1A8	FC
TotalSeq™-A0015 anti-mouse Ly-6G	1A8	PG
TotalSeq™-C0015 anti-mouse Ly-6G	1A8	PG
TotalSeq™-B0015 anti-mouse Ly-6G	1A8	PG
Spark Blue™ 550 anti-mouse Ly-6G	1A8	FC
Spark NIR™ 685 anti-mouse Ly-6G	1A8	FC
Spark YG™ 593 anti-mouse Ly-6G	1A8	FC
APC/Fire™ 810 anti-mouse Ly-6G Antibody	1A8	FC
PE/Cyanine5 anti-mouse Ly-6G	1A8	FC
PE/Fire™ 810 anti-mouse Ly-6G Antibody	1A8	FC
Spark UV™ 387 anti-mouse Ly-6G	1A8	FC
PE/Fire™ 640 anti-mouse Ly-6G	1A8	FC
Spark YG™ 570 anti-mouse Ly-6G	1A8	IHC-F,FC

Certificate of Analysis

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Go To Top Version: 3 Revision Date: 07/21/2022

For Research Use Only. Not for diagnostic or therapeutic use.

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Your actual data may vary due to variations in samples, target cells, instruments and their settings, staining conditions, and other factors.
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