Purified anti-mouse IL-22 Antibody

Pricing & Availability
BL35175 (See other available formats)
Regulatory Status
Other Names
Interleukin-22, IL-TIF, IL-10-related T-cell-derived-inducible factor
Rat IgG2a, κ
Ave. Rating
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Product Citations
Western blot analysis of 50 ng of recombinant human IL-22 (lane 1) and 50 ng of recombinant mouse IL-22 (lane 2) using purified anti-mouse IL-22 (clone BL35175) antibody.
  • BL35175_PURE_IL22_Antibody_WB_050416
    Western blot analysis of 50 ng of recombinant human IL-22 (lane 1) and 50 ng of recombinant mouse IL-22 (lane 2) using purified anti-mouse IL-22 (clone BL35175) antibody.
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517501 25 µg 100€
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517502 100 µg 212€
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IL-22, a cytokine structurally related to IL-10, was originally identified in the mouse as a gene induced by IL-9 in T cells and mast cells. IL-22 was initially designated as IL-TIF (IL10-related T cell-derived inducible factor). Mouse IL-TIF consists of 179 amino acids, including four cysteins, and has a predicted molecular weight of 20 kD.  Biological activity of IL-22 is initiated by binding to a cell-surface complex composed of IL-22R1 and IL-10R2 receptor chains and further regulated by interactions with a soluble binding protein, IL-22BP, which shares sequence similarity with an extracellular region of IL-22R1 (sIL-22R1). Both chains of the IL-22R complex belong to the class II cytokine receptor families. Two types of IL-22-binding receptor have been discovered, a membrane-bound receptor and a soluble receptor, both encoded by different genes. IL-22 is produced by immune cells and acts on non-immune cells to regulate local tissue inflammation. As a product of the recently identified T helper 17 lineage of CD4(+) effector lymphocytes, IL-22 plays a critical role in mucosal immunity as well as in dysregulated inflammation observed in autoimmune diseases.

Product Details
Technical Data Sheet (pdf)

Product Details

Verified Reactivity
Mouse, Human
Antibody Type
Host Species
Mature recombinant protein mouse Interleukin-22 (Leu34-Val179)
This antibody is provided in phosphate-buffered solution, pH 7.2, 0.09% sodium azide.
The antibody was purified by affinity chromatography.
0.5 mg/ml
Storage & Handling
Upon receipt, store undiluted between 2°C and 8°C.

WB - Quality tested

Recommended Usage

Each lot of this antibody is quality control tested by Western blotting. Western blotting, suggested working dilution(s): Use 0.5 µg/ml dilution buffer for each mini-gel.

Application Notes

Clone BL35175 has been shown to be useful for detecting mouse and human IL-22 by Western blotting.

Product Citations
  1. Ogita T, et al. 2021. Front Nutr. 8:701466. PubMed
  2. Ke Y, et al. 2011. J Immunol. 187:2130. PubMed
  3. Jiao S, et al. 2020. Cell. 179(5):1177-1190.e13.. PubMed
AB_10695475 (BioLegend Cat. No. 517501)
AB_10694243 (BioLegend Cat. No. 517502)

Antigen Details

179 aa with with a putative 33 aa signal peptide that is cleaved to generate a 147 aa mature protein.

Secreted, Th17

Cytokine that contributes to the inflammatory response in vivo.
Binds to IL-22 receptor complex that consists of two receptor subunits, IL22R and IL10Rβ.
Cell Type
Biology Area
Cell Biology, Signal Transduction
Molecular Family
Antigen References

1. Dumoutier L, et al. 2000. J. Immunol. 164:1814.
2. Xie MH, et al. 2000. J. Biol. Chem. 275:31335.
3. Jones BC, et al. 2008. Structure 16:1333.
4. Sonnenberg GF, et al. 2010. J. Exp. Med. 207:1293.
5. Kotenko SV, et al. 2001. J. Immunol. 166:7096.
6. Chang C, et al. 2006. Cell Research 16:902.

Gene ID
50929 View all products for this Gene ID
View information about IL-22 on UniProt.org

Related FAQs

There are no FAQs for this product.
Go To Top Version: 3    Revision Date: 12/14/2021

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  • Purified anti-mouse IL-22


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