Purified anti-mouse I-A/I-E Antibody

Pricing & Availability
Clone
M5/114.15.2 (See other available formats)
Regulatory Status
RUO
Other Names
MHC class II
Isotype
Rat IgG2b, κ
Ave. Rating
Submit a Review
Product Citations
publications
M5/114.15.2
C57/B6 mouse splenocytes were stained with purified anti-mouse I-A/I-E (clone M5/114.15.2) (solid line) or purified rat IgG2b, κ isotype control (dashed line) followed by IgG-FITC.
  • M5/114.15.2
    C57/B6 mouse splenocytes were stained with purified anti-mouse I-A/I-E (clone M5/114.15.2) (solid line) or purified rat IgG2b, κ isotype control (dashed line) followed by IgG-FITC.
  • M5slash114_Pure_I-A-I-E_Antibody_102518
    Fresh, frozen mouse spleen was stained with purified I-A/I-E clone M5/114.15.2 conjugated and detected with a FITC CODEX™ oligonucleotide duplex (red). Samples were counterstained with F4/80 Cy5 (green). Data generated at Akoya Biosciences, Inc. using the CODEX™ technology.
Compare all formats
Cat # Size Price Quantity Check Availability Save
107601 50 µg 52€
Check Availability


Need larger quantities of this item?
Request Bulk Quote
107602 500 µg 202€
Check Availability


Need larger quantities of this item?
Request Bulk Quote
Description

These class II molecules are expressed on antigen presenting cells (including B cells) and a subset of T cells from H-2b,d,q,r bearing mice and are involved in antigen presentation to T cells expressing CD3/TCR and CD4 proteins.

Product Details
Technical Data Sheet (pdf)

Product Details

Verified Reactivity
Mouse
Antibody Type
Monoclonal
Host Species
Rat
Immunogen
Activated C57BL/6 mouse spleen cells
Formulation
Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide.
Preparation
The antibody was purified by affinity chromatography.
Concentration
0.5 mg/ml
Storage & Handling
The antibody solution should be stored undiluted between 2°C and 8°C.
Application

FC - Quality tested
IHC-F - Verified
IP, Block - Reported in the literature, not verified in house

Recommended Usage

Each lot of this antibody is quality control tested by immunofluorescent staining with flow cytometric analysis. For flow cytometric staining, the suggested use of this reagent is ≤ 0.25 µg per million cells in 100 µl volume. It is recommended that the reagent be titrated for optimal performance for each application.

Application Notes

The M5/114.15.2 antibody reacts with a polymorphic determinant shared by the I-Ab, I-Ad, I-Aq, I-Ed, and I-Ek MHC class II alloantigens from mice carrying H-2p,r,q,b,d,u haplotypes. Clone M5/114.15.2 however does not react wtih I-Af, I-Ak, or I-As MHC class II alloantigens.1

Additional reported applications (for the relevant formats) include: immunoprecipitation1, immunohistochemistry of frozen sections2,3,6, in vitro and in vivo blocking of antigen presentation or ligand binding4-7, and spatial biology (IBEX)17,18. The Ultra-LEAF™ purified antibody (Endotoxin < 0.01 EU/µg, Azide-Free, 0.2 µm filtered) is recommended for functional assays (Cat. Nos. 107655 & 107656).

Application References

(PubMed link indicates BioLegend citation)
  1. Bhattacharya A, et al. 1981. J. Immunol. 127:2488. (IP)
  2. Viville S, et al. 1993. Cell 72:635. (IHC)
  3. Nelson AJ, et al. 1993. J. Immunol. 151:2453. (IHC)
  4. Shi Y, et al. 1998. J. Exp. Med. 187:367. (Block)
  5. Yamashita I, et al. 1993. Int. Immunol. 5:1139.
  6. Guo M, et al. 1995. Zygote 3:65. (IHC)
  7. Kim A, et al. 2004. Exp. Mol. Med. 36:428. (Block)
  8. Luckashenak NA, et al. 2006. J. Immunol. 177:5177.
  9. Venanzi ES, et al. 2007. J. Immunol. 179:5693.
  10. Christensen SR, et al. 2006. Immunity 25:417. PubMed
  11. Matte-Martone C, et al. 2008. Blood 111:3884. PubMed
  12. De Pascalis R, et al. 2008. Infect. Immun. 76:4311. PubMed
  13. Kuns RD, et al. 2009. Blood 113:5999. PubMed
  14. Sabatino JJ, et al. 2011. J. Exp. Med. 208:81. PubMed
  15. Draber P, et al. 2011. Mol Cell Biol. 22:4550. PubMed
  16. Fu H, et al. 2014. Nat Commun. 5:3436. PubMed
  17. Radtke AJ, et al. 2020. Proc Natl Acad Sci U S A. 117:33455-65. (SB) PubMed
  18. Radtke AJ, et al. 2022. Nat Protoc. 17:378-401. (SB) PubMed
Product Citations
  1. Farkas AM, et al. 2019. J Allergy Clin Immunol. 143:335. PubMed
  2. Arnold IC, et al. 2019. PLoS Pathog. 15:e1007866. PubMed
  3. Sabatino J, et al. 2011. J Exp Med. 208:81. PubMed
  4. Daemen S, et al. 2021. Cell Reports. 34(2):108626. PubMed
  5. Zaman R, et al. 2021. Immunity. :. PubMed
  6. Paterson MJ, et al. 2020. PLoS Pathog. 16:e1008733. PubMed
  7. Sorrelle N, et al. 2019. J Immunol. 202:292. PubMed
  8. Mazor R, et al. 2018. Proc Natl Acad Sci U S A. 115:E733. PubMed
  9. Jung J, et al. 2019. Cell Host Microbe. 25:513. PubMed
  10. Lau SF, et al. 2020. Cell Rep. 31:107530. PubMed
  11. Bajaña S, et al. 2021. Front Immunol. 11:577718. PubMed
  12. Bachran C, et al. 2017. Sci Rep.. 10.1038/s41598-017-17948-0. PubMed
  13. Wang W, et al. 2018. Cancer Cell. 34:757. PubMed
  14. Sparber F, et al. 2018. PLoS Pathog. 14:e1007069. PubMed
  15. Arnold IC, et al. 2018. J Exp Med. 215:2055. PubMed
  16. Perez-Muñoz M, et al. 2015. PLoS One. 10: 0144382. PubMed
  17. Prado C, et al. 2021. J Neuroinflammation. 18:292. PubMed
  18. Bonnefoy F, et al. 2018. Front Immunol. 2.170833333. PubMed
  19. Cao Q, et al. 2018. Am J Physiol Renal Physiol. 314:F561. PubMed
  20. Chikina AS, et al. 2020. Cell. 183:411. PubMed
  21. Mayer JU, et al. 2020. Front Immunol. 11:592325. PubMed
  22. Nagatake T, et al. 2022. Mucosal Immunol. 15:289. PubMed
  23. Fiala GJ, et al. 2019. J Immunol. 203:569. PubMed
  24. Claser C, et al. 2019. Nat Commun. 3.361805556. PubMed
  25. Mouat IC, et al. 2021. Elife. 10:. PubMed
  26. Stefanescu C, et al. 2021. Front Oncol. 11:765151. PubMed
  27. Kuns R, et al. 2009. Blood. 113:5999. PubMed
  28. Hitchcock JR, et al. 2020. FEBS J. 287:250. PubMed
  29. McLeod RL, et al. 2018. Oncotarget. 9:34459. PubMed
  30. Nakamizo S,et al. 2017. Sci Rep. . 10.1038/s41598-017-14292-1. PubMed
  31. Yang M, et al. 2020. Oncoimmunology. 9:1708064. PubMed
  32. Toulmin SA, et al. 2021. Nat Commun. 12:3993. PubMed
  33. Park S, et al. 2021. Nat Cell Biol. 23:476. PubMed
  34. Zhao J, et al. 2012. J Virol. 86:11416. PubMed
  35. O'Dea KP, et al. 2020. J Extracell Vesicles. 9:1706708. PubMed
  36. Hausmann A, et al. 2021. J Exp Med. 218:. PubMed
  37. Hilligan KL, et al. 2020. Nat Commun. 11:5637. PubMed
  38. Chen L, et al. 2020. Front Immunol. 11:584458. PubMed
  39. Stacey MA, et al. 2017. J Clin Invest. 127:1463. PubMed
  40. McAlpine CS, et al. 2021. Nature. 595:701. PubMed
  41. Bonnardel J, et al. 2019. Immunity. 51:638. PubMed
  42. Gartlan KH, et al. 2019. Blood Adv. 2.110416667. PubMed
  43. Abboud G, et al. 2018. Front Immunol. 9:1973. PubMed
  44. Donati Y, et al. 2020. Am J Physiol Lung Cell Mol Physiol. L619:318. PubMed
  45. Lai JF, et al. 2020. J Allergy Clin Immunol. 146:1406. PubMed
  46. Wei S, et al. 2019. Virulence. 0.588888889. PubMed
  47. Hoves S, et al. 2018. J Exp Med. 215:859. PubMed
  48. Hu G, et al. 2021. Nat Commun. 12:773. PubMed
  49. Enders M, et al. 2020. J Immunol. 204:87. PubMed
  50. Dai Z, et al. 2021. JCI Insight. 6: . PubMed
  51. Ho NI, et al. 2021. Immunology. 164:494. PubMed
  52. Krueger A, et al. 2022. Front Microbiol. 12:789042. PubMed
  53. Schuler CF, et al. 2020. Allergy. 75:2279. PubMed
  54. Matte-Martone C, et al. 2008. Blood. 111:3884. PubMed
  55. Pascalis R, et al. 2008. Infect Immun. 76:4331. PubMed
  56. Luckashenak N, et al. 2006. J Immunol. 177:5177. PubMed
  57. King IL, et al. 2017. Mucosal Immunol. 10:1160. PubMed
  58. Biton M et al. 2018. Cell. 175(5):1307-1320 . PubMed
  59. Gubin MM, et al. 2018. Cell. 175:1014. PubMed
  60. Fino KK, et al. 2017. Sci Rep. 5.334722222. PubMed
  61. Ford J, et al. 2019. Front Immunol. 2.502083333. PubMed
  62. Hoffmann FM, et al. 2018. Front Immunol. 9:1046. PubMed
  63. Li J, et al. 2021. Front Immunol. 12:710406. PubMed
  64. Kim D, et al. 2019. Immune Netw. 19:e32. PubMed
  65. Rosenbaum M, et al. 2019. Nat Commun. 2.05. PubMed
  66. Heyman O, et al. 2018. Front Immunol. 1.563888889. PubMed
  67. Zhang J, et al. 2019. J Clin Invest. 130:. PubMed
  68. Hirata SI, et al. 2020. Allergy. 75:1939. PubMed
  69. Campesato LF, et al. 2020. Nat Commun. 3.24375. PubMed
  70. Grubiši? V, et al. 2020. Cell Rep. 32:108100. PubMed
  71. Goh W, et al. 2020. Cell Rep. 33:108285. PubMed
  72. Wieghofer P, et al. 2021. EMBO J. 40:e105123. PubMed
  73. Li J, et al. 2020. Development. . PubMed
  74. Hacini–Rachinel F, et al. 2018. J Allergy Clin Immunol. 141:163. PubMed
  75. Roussey JA, et al. 2017. J Immunol. 199:3535. PubMed
  76. Jux B, et al. 2013. Int Immunol. 25:307. PubMed
  77. König S, et al. 2014. PLoS One. 9:93555. PubMed
  78. Inclan-Rico JM, et al. 2020. PLoS Pathog. 16:e1008579. PubMed
  79. Simmons S et al. 2019. Elife. 8 pii: e41239. PubMed
  80. Asadi Shahmirzadi A, et al. 2020. Cell Metabolism. 32(3):447-456.e6. PubMed
  81. Phan TS, et al. 2021. Sci Adv. 7:. PubMed
  82. Ipseiz N, et al. 2020. EMBO J. 39:e103454. PubMed
  83. Cané S, et al. 2021. J Immunother Cancer. 9:. PubMed
  84. Lee MC, et al. 2017. J Immunol. 198:3565. PubMed
  85. Diao J, et al. 2018. J Immunol. 201:1306. PubMed
  86. Pushalkar S, et al. 2018. Cancer Discov. 0.613194444. PubMed
  87. Chen B, et al. 2021. Cell. 184:6262. PubMed
  88. Maller O, et al. 2020. Nat Mater. 20:548. PubMed
  89. An Y et al. 2018. Cell reports. 25(3):702-714 . PubMed
  90. Rios–Doria J, et al. 2017. Cancer Res. 77:2686. PubMed
  91. Schneppenheim J, et al. 2017. J Immunol. 199:172. PubMed
  92. Zaric M, et al. 2017. J Control Release. 268:166. PubMed
  93. Khameneh HJ, et al. 2017. J Immunol. 198:196. PubMed
  94. Fu H, et al. 2014. Nat Commun. 5:3436. PubMed
  95. Hassel C, et al. 2021. Front Immunol. 12:754661. PubMed
  96. Song M, et al. 2020. Nat Commun. 11:6298. PubMed
  97. Wu W, et al. 2017. Sci Rep. 7:44481. PubMed
  98. Scheyltjens I, et al. 2022. Nat Protoc. 17:2354. PubMed
  99. Kästele V, et al. 2021. Mucosal Immunol. 14:717. PubMed
RRID
AB_313316 (BioLegend Cat. No. 107601)
AB_313317 (BioLegend Cat. No. 107602)

Antigen Details

Structure
MHC class II
Distribution

B cell and activated T cells, APCs of the H-2b,d,q,r bearing mice

Function
Antigen presentation
Ligand/Receptor
CD3/TCR, CD4
Cell Type
Antigen-presenting cells, B cells, Dendritic cells, T cells, Tregs
Biology Area
Immunology, Innate Immunity
Molecular Family
MHC Antigens
Antigen References

1. Watts C. 1997. Ann. Rev. Immunol. 15:821.
2. Pamer E, et al. 1998. Ann. Rev. Immunol. 16:323.

Gene ID
14961 View all products for this Gene ID 14969 View all products for this Gene ID
UniProt
View information about I-A/I-E on UniProt.org

Related FAQs

There are no FAQs for this product.

Other Formats

View All I-A/I-E Reagents Request Custom Conjugation
Description Clone Applications
Biotin anti-mouse I-A/I-E M5/114.15.2 FC
FITC anti-mouse I-A/I-E M5/114.15.2 FC
PE anti-mouse I-A/I-E M5/114.15.2 FC
Purified anti-mouse I-A/I-E M5/114.15.2 FC, IHC-F, IP, Block
PE/Cyanine5 anti-mouse I-A/I-E M5/114.15.2 FC
APC anti-mouse I-A/I-E M5/114.15.2 FC
Alexa Fluor® 488 anti-mouse I-A/I-E M5/114.15.2 FC, 3D IHC
Alexa Fluor® 647 anti-mouse I-A/I-E M5/114.15.2 FC
Pacific Blue™ anti-mouse I-A/I-E M5/114.15.2 FC
Alexa Fluor® 700 anti-mouse I-A/I-E M5/114.15.2 FC, SB
PerCP/Cyanine5.5 anti-mouse I-A/I-E M5/114.15.2 FC
PerCP anti-mouse I-A/I-E M5/114.15.2 FC
APC/Cyanine7 anti-mouse I-A/I-E M5/114.15.2 FC
PE/Cyanine7 anti-mouse I-A/I-E M5/114.15.2 FC
Brilliant Violet 421™ anti-mouse I-A/I-E M5/114.15.2 FC, IHC-F, SB
Brilliant Violet 510™ anti-mouse I-A/I-E M5/114.15.2 FC
Purified anti-mouse I-A/I-E (Maxpar® Ready) M5/114.15.2 FC, CyTOF®
Brilliant Violet 605™ anti-mouse I-A/I-E M5/114.15.2 FC
Brilliant Violet 650™ anti-mouse I-A/I-E M5/114.15.2 FC
Brilliant Violet 711™ anti-mouse I-A/I-E M5/114.15.2 FC
Brilliant Violet 785™ anti-mouse I-A/I-E M5/114.15.2 FC
PE/Dazzle™ 594 anti-mouse I-A/I-E M5/114.15.2 FC
Alexa Fluor® 594 anti-mouse I-A/I-E M5/114.15.2 IHC-F, 3D IHC
APC/Fire™ 750 anti-mouse I-A/I-E M5/114.15.2 FC
TotalSeq™-A0117 anti-mouse I-A/I-E M5/114.15.2 PG
Ultra-LEAF™ Purified anti-mouse I-A/I-E M5/114.15.2 FC, IHC-F, IP, Block
TotalSeq™-B0117 anti-mouse I-A/I-E M5/114.15.2 PG
TotalSeq™-C0117 anti-mouse I-A/I-E M5/114.15.2 PG
Spark Blue™ 550 anti-mouse I-A/I-E M5/114.15.2 FC
PE/Fire™ 640 anti-mouse I-A/I-E M5/114.15.2 FC
Spark YG™ 581 anti-mouse I-A/I-E M5/114.15.2 FC
PE/Fire™ 810 anti-mouse I-A/I-E M5/114.15.2 FC
Spark UV™ 387 anti-mouse I-A/I-E M5/114.15.2 FC
Go To Top Version: 5    Revision Date: 10/25/2018

For research use only. Not for diagnostic use. Not for resale. BioLegend will not be held responsible for patent infringement or other violations that may occur with the use of our products.

 

*These products may be covered by one or more Limited Use Label Licenses (see the BioLegend Catalog or our website, www.biolegend.com/ordering#license). BioLegend products may not be transferred to third parties, resold, modified for resale, or used to manufacture commercial products, reverse engineer functionally similar materials, or to provide a service to third parties without written approval of BioLegend. By use of these products you accept the terms and conditions of all applicable Limited Use Label Licenses. Unless otherwise indicated, these products are for research use only and are not intended for human or animal diagnostic, therapeutic or commercial use.

 

BioLegend Inc., 8999 BioLegend Way, San Diego, CA 92121 www.biolegend.com
Toll-Free Phone: 1-877-Bio-Legend (246-5343) Phone: (858) 768-5800 Fax: (877) 455-9587

This data display is provided for general comparisons between formats.
Your actual data may vary due to variations in samples, target cells, instruments and their settings, staining conditions, and other factors.
If you need assistance with selecting the best format contact our expert technical support team.

  • Biotin anti-mouse I-A/I-E

  • FITC anti-mouse I-A/I-E

  • PE anti-mouse I-A/I-E

  • Purified anti-mouse I-A/I-E

  • PE/Cyanine5 anti-mouse I-A/I-E

  • APC anti-mouse I-A/I-E

  • Alexa Fluor® 488 anti-mouse I-A/I-E

  • Alexa Fluor® 647 anti-mouse I-A/I-E

  • Pacific Blue™ anti-mouse I-A/I-E

  • Alexa Fluor® 700 anti-mouse I-A/I-E

  • PerCP/Cyanine5.5 anti-mouse I-A/I-E

  • PerCP anti-mouse I-A/I-E

  • APC/Cyanine7 anti-mouse I-A/I-E

  • PE/Cyanine7 anti-mouse I-A/I-E

  • Brilliant Violet 421™ anti-mouse I-A/I-E

  • Brilliant Violet 510™ anti-mouse I-A/I-E

  • Purified anti-mouse I-A/I-E (Maxpar® Ready)

  • Brilliant Violet 605™ anti-mouse I-A/I-E

  • Brilliant Violet 650™ anti-mouse I-A/I-E

  • Brilliant Violet 711™ anti-mouse I-A/I-E

  • Brilliant Violet 785™ anti-mouse I-A/I-E

  • PE/Dazzle™ 594 anti-mouse I-A/I-E

  • Alexa Fluor® 594 anti-mouse I-A/I-E

  • APC/Fire™ 750 anti-mouse I-A/I-E

  • TotalSeq™-A0117 anti-mouse I-A/I-E

  • Ultra-LEAF™ Purified anti-mouse I-A/I-E

  • TotalSeq™-B0117 anti-mouse I-A/I-E

  • TotalSeq™-C0117 anti-mouse I-A/I-E

  • Spark Blue™ 550 anti-mouse I-A/I-E

  • PE/Fire™ 640 anti-mouse I-A/I-E

  • Spark YG™ 581 anti-mouse I-A/I-E

  • PE/Fire™ 810 anti-mouse I-A/I-E

  • Spark UV™ 387 anti-mouse I-A/I-E

ProductsHere

Login / Register
Remember me
Forgot your password? Reset password?
Create an Account