Purified anti-mouse I-A/I-E Antibody

Pricing & Availability
Clone
M5/114.15.2 (See other available formats)
Regulatory Status
RUO
Other Names
MHC class II
Isotype
Rat IgG2b, κ
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Product Citations
publications
M5/114.15.2
C57/B6 mouse splenocytes were stained with purified anti-mouse I-A/I-E (clone M5/114.15.2) (solid line) or purified rat IgG2b, κ isotype control (dashed line) followed by IgG-FITC.
  • M5/114.15.2
    C57/B6 mouse splenocytes were stained with purified anti-mouse I-A/I-E (clone M5/114.15.2) (solid line) or purified rat IgG2b, κ isotype control (dashed line) followed by IgG-FITC.
  • M5slash114_Pure_I-A-I-E_Antibody_102518
    Fresh, frozen mouse spleen was stained with purified I-A/I-E clone M5/114.15.2 conjugated and detected with a FITC CODEX™ oligonucleotide duplex (red). Samples were counterstained with F4/80 Cy5 (green). Data generated at Akoya Biosciences, Inc. using the CODEX™ technology.
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107601 50 µg 52€
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107602 500 µg 202€
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Description

These class II molecules are expressed on antigen presenting cells (including B cells) and a subset of T cells from H-2b,d,q,r bearing mice and are involved in antigen presentation to T cells expressing CD3/TCR and CD4 proteins.

Product Details
Technical Data Sheet (pdf)

Product Details

Verified Reactivity
Mouse
Antibody Type
Monoclonal
Host Species
Rat
Immunogen
Activated C57BL/6 mouse spleen cells
Formulation
Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide.
Preparation
The antibody was purified by affinity chromatography.
Concentration
0.5 mg/ml
Storage & Handling
The antibody solution should be stored undiluted between 2°C and 8°C.
Application

FC - Quality tested
IHC-F - Verified
IP, Block - Reported in the literature, not verified in house

Recommended Usage

Each lot of this antibody is quality control tested by immunofluorescent staining with flow cytometric analysis. For flow cytometric staining, the suggested use of this reagent is ≤ 0.25 µg per million cells in 100 µl volume. It is recommended that the reagent be titrated for optimal performance for each application.

Application Notes

The M5/114.15.2 antibody reacts with a polymorphic determinant shared by the I-Ab, I-Ad, I-Aq, I-Ed, and I-Ek MHC class II alloantigens from mice carrying H-2p,r,q,b,d,u haplotypes. Clone M5/114.15.2 however does not react wtih I-Af, I-Ak, or I-As MHC class II alloantigens.1

Additional reported applications (for the relevant formats) include: immunoprecipitation1, immunohistochemistry of frozen sections2,3,6, in vitro and in vivo blocking of antigen presentation or ligand binding4-7, and spatial biology (IBEX)17,18. The Ultra-LEAF™ purified antibody (Endotoxin < 0.01 EU/µg, Azide-Free, 0.2 µm filtered) is recommended for functional assays (Cat. Nos. 107655 & 107656).

Application References

(PubMed link indicates BioLegend citation)
  1. Bhattacharya A, et al. 1981. J. Immunol. 127:2488. (IP)
  2. Viville S, et al. 1993. Cell 72:635. (IHC)
  3. Nelson AJ, et al. 1993. J. Immunol. 151:2453. (IHC)
  4. Shi Y, et al. 1998. J. Exp. Med. 187:367. (Block)
  5. Yamashita I, et al. 1993. Int. Immunol. 5:1139.
  6. Guo M, et al. 1995. Zygote 3:65. (IHC)
  7. Kim A, et al. 2004. Exp. Mol. Med. 36:428. (Block)
  8. Luckashenak NA, et al. 2006. J. Immunol. 177:5177.
  9. Venanzi ES, et al. 2007. J. Immunol. 179:5693.
  10. Christensen SR, et al. 2006. Immunity 25:417. PubMed
  11. Matte-Martone C, et al. 2008. Blood 111:3884. PubMed
  12. De Pascalis R, et al. 2008. Infect. Immun. 76:4311. PubMed
  13. Kuns RD, et al. 2009. Blood 113:5999. PubMed
  14. Sabatino JJ, et al. 2011. J. Exp. Med. 208:81. PubMed
  15. Draber P, et al. 2011. Mol Cell Biol. 22:4550. PubMed
  16. Fu H, et al. 2014. Nat Commun. 5:3436. PubMed
  17. Radtke AJ, et al. 2020. Proc Natl Acad Sci U S A. 117:33455-65. (SB) PubMed
  18. Radtke AJ, et al. 2022. Nat Protoc. 17:378-401. (SB) PubMed
Product Citations
  1. Paterson MJ, et al. 2020. PLoS Pathog. 16:e1008733. PubMed
  2. Cai S, et al. 2020. Sci Rep. 10:14249. PubMed
  3. Malinczak CA, et al. 2021. J Immunol. 206:1315. PubMed
  4. Li H, et al. 2022. Cancer Cell. 40:36. PubMed
  5. Campisi L, et al. 2022. Nature. 606:945. PubMed
  6. Han G, et al. 2022. Gut Microbes. 14:2104086. PubMed
  7. Moore AR, et al. 2022. Cell Rep. 41:111651. PubMed
  8. Daemen S, et al. 2021. Cell Reports. 34(2):108626. PubMed
  9. Zaman R, et al. 2021. Immunity. :. PubMed
  10. Farkas AM, et al. 2019. J Allergy Clin Immunol. 143:335. PubMed
  11. Arnold IC, et al. 2019. PLoS Pathog. 15:e1007866. PubMed
  12. Sabatino J, et al. 2011. J Exp Med. 208:81. PubMed
  13. Liu HJ, et al. 2023. Nat Commun. 14:1214. PubMed
  14. Bajaña S, et al. 2021. Front Immunol. 11:577718. PubMed
  15. Lau SF, et al. 2020. Cell Rep. 31:107530. PubMed
  16. Sorrelle N, et al. 2019. J Immunol. 202:292. PubMed
  17. Mazor R, et al. 2018. Proc Natl Acad Sci U S A. 115:E733. PubMed
  18. Jung J, et al. 2019. Cell Host Microbe. 25:513. PubMed
  19. Prado C, et al. 2021. J Neuroinflammation. 18:292. PubMed
  20. Perez-Muñoz M, et al. 2015. PLoS One. 10: 0144382. PubMed
  21. Bachran C, et al. 2017. Sci Rep.. 10.1038/s41598-017-17948-0. PubMed
  22. Wang W, et al. 2018. Cancer Cell. 34:757. PubMed
  23. Sparber F, et al. 2018. PLoS Pathog. 14:e1007069. PubMed
  24. Arnold IC, et al. 2018. J Exp Med. 215:2055. PubMed
  25. Mayer JU, et al. 2020. Front Immunol. 11:592325. PubMed
  26. Nagatake T, et al. 2022. Mucosal Immunol. 15:289. PubMed
  27. Chikina AS, et al. 2020. Cell. 183:411. PubMed
  28. Bonnefoy F, et al. 2018. Front Immunol. 2.170833333. PubMed
  29. Cao Q, et al. 2018. Am J Physiol Renal Physiol. 314:F561. PubMed
  30. Mouat IC, et al. 2021. Elife. 10:. PubMed
  31. Stefanescu C, et al. 2021. Front Oncol. 11:765151. PubMed
  32. Fiala GJ, et al. 2019. J Immunol. 203:569. PubMed
  33. Claser C, et al. 2019. Nat Commun. 3.361805556. PubMed
  34. Yang M, et al. 2020. Oncoimmunology. 9:1708064. PubMed
  35. Toulmin SA, et al. 2021. Nat Commun. 12:3993. PubMed
  36. Park S, et al. 2021. Nat Cell Biol. 23:476. PubMed
  37. Kuns R, et al. 2009. Blood. 113:5999. PubMed
  38. Hitchcock JR, et al. 2020. FEBS J. 287:250. PubMed
  39. McLeod RL, et al. 2018. Oncotarget. 9:34459. PubMed
  40. Nakamizo S,et al. 2017. Sci Rep. . 10.1038/s41598-017-14292-1. PubMed
  41. O'Dea KP, et al. 2020. J Extracell Vesicles. 9:1706708. PubMed
  42. Hausmann A, et al. 2021. J Exp Med. 218:. PubMed
  43. Hilligan KL, et al. 2020. Nat Commun. 11:5637. PubMed
  44. Chen L, et al. 2020. Front Immunol. 11:584458. PubMed
  45. Zhao J, et al. 2012. J Virol. 86:11416. PubMed
  46. McAlpine CS, et al. 2021. Nature. 595:701. PubMed
  47. Stacey MA, et al. 2017. J Clin Invest. 127:1463. PubMed
  48. Lai JF, et al. 2020. J Allergy Clin Immunol. 146:1406. PubMed
  49. Donati Y, et al. 2020. Am J Physiol Lung Cell Mol Physiol. L619:318. PubMed
  50. Bonnardel J, et al. 2019. Immunity. 51:638. PubMed
  51. Gartlan KH, et al. 2019. Blood Adv. 2.110416667. PubMed
  52. Abboud G, et al. 2018. Front Immunol. 9:1973. PubMed
  53. Hu G, et al. 2021. Nat Commun. 12:773. PubMed
  54. Enders M, et al. 2020. J Immunol. 204:87. PubMed
  55. Dai Z, et al. 2021. JCI Insight. 6: . PubMed
  56. Wei S, et al. 2019. Virulence. 0.588888889. PubMed
  57. Hoves S, et al. 2018. J Exp Med. 215:859. PubMed
  58. Ho NI, et al. 2021. Immunology. 164:494. PubMed
  59. Krueger A, et al. 2022. Front Microbiol. 12:789042. PubMed
  60. Schuler CF, et al. 2020. Allergy. 75:2279. PubMed
  61. Matte-Martone C, et al. 2008. Blood. 111:3884. PubMed
  62. Pascalis R, et al. 2008. Infect Immun. 76:4331. PubMed
  63. Luckashenak N, et al. 2006. J Immunol. 177:5177. PubMed
  64. King IL, et al. 2017. Mucosal Immunol. 10:1160. PubMed
  65. Biton M et al. 2018. Cell. 175(5):1307-1320 . PubMed
  66. Li J, et al. 2021. Front Immunol. 12:710406. PubMed
  67. Gubin MM, et al. 2018. Cell. 175:1014. PubMed
  68. Fino KK, et al. 2017. Sci Rep. 5.334722222. PubMed
  69. Ford J, et al. 2019. Front Immunol. 2.502083333. PubMed
  70. Hoffmann FM, et al. 2018. Front Immunol. 9:1046. PubMed
  71. Wieghofer P, et al. 2021. EMBO J. 40:e105123. PubMed
  72. Li J, et al. 2020. Development. :. PubMed
  73. Hirata SI, et al. 2020. Allergy. 75:1939. PubMed
  74. Campesato LF, et al. 2020. Nat Commun. 3.24375. PubMed
  75. Grubiši? V, et al. 2020. Cell Rep. 32:108100. PubMed
  76. Goh W, et al. 2020. Cell Rep. 33:108285. PubMed
  77. Kim D, et al. 2019. Immune Netw. 19:e32. PubMed
  78. Rosenbaum M, et al. 2019. Nat Commun. 2.05. PubMed
  79. Heyman O, et al. 2018. Front Immunol. 1.563888889. PubMed
  80. Zhang J, et al. 2019. J Clin Invest. 130:. PubMed
  81. Inclan-Rico JM, et al. 2020. PLoS Pathog. 16:e1008579. PubMed
  82. Jux B, et al. 2013. Int Immunol. 25:307. PubMed
  83. König S, et al. 2014. PLoS One. 9:93555. PubMed
  84. Hacini–Rachinel F, et al. 2018. J Allergy Clin Immunol. 141:163. PubMed
  85. Roussey JA, et al. 2017. J Immunol. 199:3535. PubMed
  86. Phan TS, et al. 2021. Sci Adv. 7:. PubMed
  87. Asadi Shahmirzadi A, et al. 2020. Cell Metabolism. 32(3):447-456.e6. PubMed
  88. Simmons S et al. 2019. Elife. 8 pii: e41239. PubMed
  89. Ipseiz N, et al. 2020. EMBO J. 39:e103454. PubMed
  90. Cané S, et al. 2021. J Immunother Cancer. 9:. PubMed
  91. Chen B, et al. 2021. Cell. 184:6262. PubMed
  92. Maller O, et al. 2020. Nat Mater. 20:548. PubMed
  93. Lee MC, et al. 2017. J Immunol. 198:3565. PubMed
  94. Diao J, et al. 2018. J Immunol. 201:1306. PubMed
  95. Pushalkar S, et al. 2018. Cancer Discov. 0.613194444. PubMed
  96. Hassel C, et al. 2021. Front Immunol. 12:754661. PubMed
  97. Song M, et al. 2020. Nat Commun. 11:6298. PubMed
  98. Fu H, et al. 2014. Nat Commun. 5:3436. PubMed
  99. An Y et al. 2018. Cell reports. 25(3):702-714 . PubMed
  100. Rios–Doria J, et al. 2017. Cancer Res. 77:2686. PubMed
  101. Schneppenheim J, et al. 2017. J Immunol. 199:172. PubMed
  102. Zaric M, et al. 2017. J Control Release. 268:166. PubMed
  103. Khameneh HJ, et al. 2017. J Immunol. 198:196. PubMed
  104. Scheyltjens I, et al. 2022. Nat Protoc. 17:2354. PubMed
  105. Kästele V, et al. 2021. Mucosal Immunol. 14:717. PubMed
  106. Wu W, et al. 2017. Sci Rep. 7:44481. PubMed
RRID
AB_313316 (BioLegend Cat. No. 107601)
AB_313317 (BioLegend Cat. No. 107602)

Antigen Details

Structure
MHC class II
Distribution

B cell and activated T cells, APCs of the H-2b,d,q,r bearing mice

Function
Antigen presentation
Ligand/Receptor
CD3/TCR, CD4
Cell Type
Antigen-presenting cells, B cells, Dendritic cells, T cells, Tregs
Biology Area
Immunology, Innate Immunity
Molecular Family
MHC Antigens
Antigen References

1. Watts C. 1997. Ann. Rev. Immunol. 15:821.
2. Pamer E, et al. 1998. Ann. Rev. Immunol. 16:323.

Gene ID
14961 View all products for this Gene ID 14969 View all products for this Gene ID
UniProt
View information about I-A/I-E on UniProt.org

Related FAQs

There are no FAQs for this product.

Other Formats

View All I-A/I-E Reagents Request Custom Conjugation
Description Clone Applications
Biotin anti-mouse I-A/I-E M5/114.15.2 FC
FITC anti-mouse I-A/I-E M5/114.15.2 FC
PE anti-mouse I-A/I-E M5/114.15.2 FC
Purified anti-mouse I-A/I-E M5/114.15.2 FC,IHC-F,IP,Block
PE/Cyanine5 anti-mouse I-A/I-E M5/114.15.2 FC
APC anti-mouse I-A/I-E M5/114.15.2 FC
Alexa Fluor® 488 anti-mouse I-A/I-E M5/114.15.2 FC,3D IHC
Alexa Fluor® 647 anti-mouse I-A/I-E M5/114.15.2 FC
Pacific Blue™ anti-mouse I-A/I-E M5/114.15.2 FC
Alexa Fluor® 700 anti-mouse I-A/I-E M5/114.15.2 FC,SB
PerCP/Cyanine5.5 anti-mouse I-A/I-E M5/114.15.2 FC
PerCP anti-mouse I-A/I-E M5/114.15.2 FC
APC/Cyanine7 anti-mouse I-A/I-E M5/114.15.2 FC
PE/Cyanine7 anti-mouse I-A/I-E M5/114.15.2 FC
Brilliant Violet 421™ anti-mouse I-A/I-E M5/114.15.2 FC,IHC-F,SB
Brilliant Violet 510™ anti-mouse I-A/I-E M5/114.15.2 FC
Purified anti-mouse I-A/I-E (Maxpar® Ready) M5/114.15.2 FC,CyTOF®
Brilliant Violet 605™ anti-mouse I-A/I-E M5/114.15.2 FC
Brilliant Violet 650™ anti-mouse I-A/I-E M5/114.15.2 FC
Brilliant Violet 711™ anti-mouse I-A/I-E M5/114.15.2 FC
Brilliant Violet 785™ anti-mouse I-A/I-E M5/114.15.2 FC
PE/Dazzle™ 594 anti-mouse I-A/I-E M5/114.15.2 FC
Alexa Fluor® 594 anti-mouse I-A/I-E M5/114.15.2 IHC-F,3D IHC
APC/Fire™ 750 anti-mouse I-A/I-E M5/114.15.2 FC
TotalSeq™-A0117 anti-mouse I-A/I-E M5/114.15.2 PG
Ultra-LEAF™ Purified anti-mouse I-A/I-E M5/114.15.2 FC,IHC-F,IP,Block
TotalSeq™-B0117 anti-mouse I-A/I-E M5/114.15.2 PG
TotalSeq™-C0117 anti-mouse I-A/I-E M5/114.15.2 PG
Spark Blue™ 550 anti-mouse I-A/I-E M5/114.15.2 FC
PE/Fire™ 640 anti-mouse I-A/I-E M5/114.15.2 FC
Spark YG™ 581 anti-mouse I-A/I-E M5/114.15.2 FC
PE/Fire™ 810 anti-mouse I-A/I-E M5/114.15.2 FC
Spark UV™ 387 anti-mouse I-A/I-E M5/114.15.2 FC
Spark Violet™ 538 anti-mouse I-A/I-E M5/114.15.2 FC
PerCP/Fire™ 806 anti-mouse I-A/I-E M5/114.15.2 FC
Spark Red™ 718 anti-mouse I-A/I-E M5/114.15.2 FC
APC/Fire™ 810 anti-mouse I-A/I-E M5/114.15.2 FC
Spark PLUS UV™ 395 anti-mouse I-A/I-E M5/114.15.2 FC
Go To Top Version: 5    Revision Date: 10/25/2018

For Research Use Only. Not for diagnostic or therapeutic use.

 

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This data display is provided for general comparisons between formats.
Your actual data may vary due to variations in samples, target cells, instruments and their settings, staining conditions, and other factors.
If you need assistance with selecting the best format contact our expert technical support team.

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