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Purified anti-mouse CD63 Antibody

Pricing & Availability
Clone
NVG-2 (See other available formats)
Regulatory Status
RUO
Other Names
LIMP, LAMP-3, gp55, melanoma-associated antigen, ME491
Isotype
Rat IgG2a, κ
Ave. Rating
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Product Citations
publications
a-NVG-2_Pure_CD63_Antibody_1_040620
Thioglycolate-elicited BALB/c mouse peritoneal macrophages were fixed, permeabilized and intracellularly stained with purified CD63 (clone NVG-2) (left) or purified rat IgG2a, κ isotype control (right), followed by biotinylated anti Rat IgG, SAV PE, and CD11b APC.
  • a-NVG-2_Pure_CD63_Antibody_1_040620
    Thioglycolate-elicited BALB/c mouse peritoneal macrophages were fixed, permeabilized and intracellularly stained with purified CD63 (clone NVG-2) (left) or purified rat IgG2a, κ isotype control (right), followed by biotinylated anti Rat IgG, SAV PE, and CD11b APC.
  • b-NVG-2_Pure_041312
    bEnd.3, mouse endothelial cells were stained with purified CD63 (clone NVG-2) (filled histogram) or rat IgG2a, κ isotype control (open histogram), followed by anti-rat IgG PE.
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143901 25 µg 72€
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143902 100 µg 165€
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Description

CD63, also known as LIMP, LAMP-3, gp55, and melanoma-associated antigen (ME491), is a member of the tetraspanin superfamily (TM4SF) that constitutes a main component of the lysosomal membrane. It is expressed on activated platelets, monocyte/macrophages, endothelium, fibroblasts, osteoblasts, and smooth muscle cells. CD63 may be involved in platelet activation and is thought to function as a transmembrane adaptor protein. CD63 has been shown to associate with CD9, CD81, VLA-3, and VLA-6. In mice, there are two CD63 gene loci, of which only one is functional. CD63 deficient mice are viable, and there is no alteration in the population of immune cells.  A recent report shows that CD63-deficient mice exhibit a significant reduction in both leukocyte rolling and recruitment in a peritonitis model. 

Product Details
Technical Data Sheet (pdf)

Product Details

Verified Reactivity
Mouse
Antibody Type
Monoclonal
Host Species
Rat
Immunogen
Intestinal lamina propria light-density cells (enriched with eosinophils)
Formulation
Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide.
Preparation
The antibody was purified by affinity chromatography.
Concentration
0.5 mg/mL
Storage & Handling
The antibody solution should be stored undiluted between 2°C and 8°C.
Application

ICFC - Quality tested
FC - Verified
WB, IHC-F - Reported in the literature, not verified in house
Recommended Usage

Each lot of this antibody is quality control tested by intracellular immunofluorescent staining with flow cytometric analysis. For intracellular flow cytometric staining, the suggested use of this reagent is ≤ 0.5 µg per million cells in 100 µL volume. For flow cytometric staining, the suggested use of this reagent is ≤ 1.0 µg per million cells in 100 µL volume. It is recommended that the reagent be titrated for optimal performance for each application.

Application Notes

Additional reported applications (for the relevant formats) include: Western blotting1 and immunofluorescence1.

Application References

(PubMed link indicates BioLegend citation)
  1. Verjan Garcia N, et al. 2011. J. Immunol. 187:2268. (WB, IF)
Product Citations
  1. Ma Y, et al. 2021. Sci Rep. 11:13471. PubMed
  2. Hutton C, et al. 2021. Cancer Cell. 39:1227. PubMed
  3. Daemen S, et al. 2021. Cell Reports. 34(2):108626. PubMed
  4. Arnold IC, et al. 2018. J Exp Med. 215:2055. PubMed
  5. Crawford G, et al. 2018. Nat Immunol. 1.388194444. PubMed
  6. Wang G, et al. 2021. Nat Commun. 12:5733. PubMed
  7. Cappariello A, et al. 2018. J Bone Miner Res. 33:517. PubMed
  8. Elgawidi A, et al. 2020. Med Microbiol Immunol. 209:473. PubMed
  9. O'Dea KP, et al. 2020. J Extracell Vesicles. 9:1706708. PubMed
  10. Jiang L, et al. 2020. Microorganisms. 9:. PubMed
  11. Nakai W, et al. 2016. Sci Rep. 6: 33935. PubMed
  12. Samoylenko A, et al. 2021. Sci Rep. 11:19594. PubMed
  13. Teng Y, et al. 2017. Nat Commun. 8:14448. PubMed
  14. Daemen S, et al. 2021. STAR Protoc. 2:100511. PubMed
  15. Gubin MM, et al. 2018. Cell. 175:1014. PubMed
  16. Lobastova L, et al. 2021. Frontiers in Cell and Developmental Biology. 9:698503. PubMed
  17. Sudhakar JN, et al. 2020. Nat Commun. 3.434722222. PubMed
  18. Lloyd-Lewis B, et al. 2018. J Biol Chem. 9:2684. PubMed
  19. Degosserie J, et al. 2018. J Extracell Vesicles. 11:243. PubMed
  20. Garcia-Martin R, et al. 2022. Nature. 601:446. PubMed
  21. Teng Y, et al. 2021. Mol Ther. 29:2424. PubMed
  22. Harada Y, et al. 2020. Cell Rep. 33:108261. PubMed
  23. Garcia-Martin R, et al. 2022. Cell Rep. 38:110277. PubMed
  24. Cappariello A, et al. 2022. Calcif Tissue Int. Online ahead of print. PubMed
  25. Ruan Z, et al. 2022. Cell Rep. 39:110791. PubMed
RRID
AB_11203908 (BioLegend Cat. No. 143901)
AB_11204263 (BioLegend Cat. No. 143902)

Antigen Details

Structure
Tetraspan transmembrane superfamily (TM4SF), type III lysosomal glycoprotein, 53 kD
Distribution

Activated platelets, monocytes, macrophages, endothelium, fibroblasts, osteoclasts, and smooth muscle cells

Function
Platelet activation
Interaction
CD9, CD81, VLA-3, and VLA-6
Cell Type
Endothelial cells, Fibroblasts, Macrophages, Monocytes, Osteoclasts, Platelets
Biology Area
Immunology, Innate Immunity
Molecular Family
CD Molecules
Antigen References

1. Azorsa DO, et al. 1991. Blood 78:280.
2. Kishimoto T, et al. 1997. Leukocyte Typing V1. Oxford University Press New York.
3. Hildreth JE, et al. 1991. Blood 77:121.

Gene ID
12512 View all products for this Gene ID
UniProt
View information about CD63 on UniProt.org

Related FAQs

There are no FAQs for this product.
Go To Top Version: 3    Revision Date: 04/06/2020

For research use only. Not for diagnostic use. Not for resale. BioLegend will not be held responsible for patent infringement or other violations that may occur with the use of our products.

 

*These products may be covered by one or more Limited Use Label Licenses (see the BioLegend Catalog or our website, www.biolegend.com/ordering#license). BioLegend products may not be transferred to third parties, resold, modified for resale, or used to manufacture commercial products, reverse engineer functionally similar materials, or to provide a service to third parties without written approval of BioLegend. By use of these products you accept the terms and conditions of all applicable Limited Use Label Licenses. Unless otherwise indicated, these products are for research use only and are not intended for human or animal diagnostic, therapeutic or commercial use.

 

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This data display is provided for general comparisons between formats.
Your actual data may vary due to variations in samples, target cells, instruments and their settings, staining conditions, and other factors.
If you need assistance with selecting the best format contact our expert technical support team.

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