Purified anti-mouse CD127 (IL-7Rα) Antibody

Pricing & Availability
Clone
A7R34 (See other available formats)
Regulatory Status
RUO
Other Names
IL-7 receptor α chain, IL-7Rα
Isotype
Rat IgG2a, κ
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Product Citations
publications
A_A7R34_PE_100809
C57BL/6 mouse splenocytes stained with rat IgG2a (RTK2758) PE isotype control and CD3 (145-2C11) APC
  • A_A7R34_PE_100809
    C57BL/6 mouse splenocytes stained with rat IgG2a (RTK2758) PE isotype control and CD3 (145-2C11) APC
  • B_A7R34_PE_100809
    C57BL/6 mouse splenocytes stained with A7R34 PE and CD3 (145-2C11) APC
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135002 500 µg 109€
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Description

CD127 is a 60-90 kD type I transmembrane glycoprotein also known as IL-7 receptor α chain or IL-7Rα. It forms a heterodimer with the common γ chain (γc or CD132) which is shared with the receptors for IL-2, IL-4, IL-9, IL-13, IL-15, and IL-21. CD127 is expressed on immature B cells through early pre-B stage, thymocytes (except CD4/CD8 double positive thymocytes), peripheral T cells, and bone marrow stromal cells. CD127 has been reported to be an useful marker for identifying memory and effector T cells. The ligation of IL-7 with its receptor is important for stimulation of mature and immature T cells as well as immature B cells proliferation and development.

Product Details
Technical Data Sheet (pdf)

Product Details

Verified Reactivity
Mouse
Antibody Type
Monoclonal
Host Species
Rat
Immunogen
IL-7Ra-IgG1 fusion protein
Formulation
Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide.
Preparation
The antibody was purified by affinity chromatography.
Concentration
0.5 mg/mL
Storage & Handling
The antibody solution should be stored undiluted between 2°C and 8°C.
Application

FC - Quality tested
IP, WB, IHC, ICC - Reported in the literature, not verified in house

Recommended Usage

Each lot of this antibody is quality control tested by immunofluorescent staining with flow cytometric analysis. For flow cytometric staining, the suggested use of this reagent is ≤ 1.0 µg per million cells in 100 µL volume. It is recommended that the reagent be titrated for optimal performance for each application.

Application Notes

A7R34 is able to block clone SB/199 binding to IL-7R.

Application References

(PubMed link indicates BioLegend citation)
  1. Sudo T, et al. 1993. P. Natl. Acad. Sci. USA 90:9125.
  2. Hashi H, et al. 2001. J. Immunol. 166:3702.
  3. Taylor R, et al. 2007. J. Immunol. 178:5659.
  4. Mazzon C, et al. 2011. Blood. 118:2733. PubMed
  5. Jin J, et al. 2011. J. Immunol. doi:10.4049/jimmunol.1001238. PubMed
Product Citations
  1. Campisi L, et al. 2022. Nature. 606:945. PubMed
  2. Potempa M, et al. 2022. J Immunol. 208:1742. PubMed
  3. Khan KA, et al. 2020. NPJ Breast Cancer. 6:29. PubMed
  4. Arnold IC, et al. 2018. J Exp Med. 215:2055. PubMed
  5. De Vlaeminck Y, et al. 2020. Cancers (Basel). 12:. PubMed
  6. Nguyen N, et al. 2022. iScience. 25:103679. PubMed
  7. Carpenter SM, et al. 2017. PLoS Pathog. 13:e1006704. PubMed
  8. Shen G, et al. 2017. Eur J Immunol. 47:900. PubMed
  9. Qin Q, et al. 2017. Virology. 502:198. PubMed
  10. Morabito KM, et al. 2017. Mucosal Immunol. 0.795138889. PubMed
  11. Khan KA, et al. 2020. NPJ Breast Cancer. 6:29. PubMed
  12. Zhou D, et al. 2022. Nat Commun. 13:5174. PubMed
  13. Hsiao CC, et al. 2021. Cells. 10:. PubMed
  14. Mazzon C, et al. 2011. Blood. 118:2733. PubMed
  15. Sun Y, et al. 2020. J Immunol. 205:3358. PubMed
  16. Verbiest T, et al. 2018. Leukemia. 32:1435. PubMed
  17. Gubin MM, et al. 2018. Cell. 175:1014. PubMed
  18. Parigi SM, et al. 2018. Sci Rep. 0.440277778. PubMed
  19. Karmaus PWF, et al. 2019. Nature. 565:101. PubMed
  20. Neumann K, et al. 2017. J Immunol. 198(1):128-137. PubMed
  21. Kakaradov B, et al. 2017. Nat Immunol. 18:422. PubMed
  22. Inclan-Rico JM, et al. 2020. PLoS Pathog. 16:e1008579. PubMed
  23. Link CWM, et al. 2020. Front Immunol. 11:596772. PubMed
  24. Wagner AK, et al. 2017. Nat Commun. 8:15627. PubMed
  25. Stelekati E, et al. 2018. Cell Rep. 2.445833333. PubMed
  26. Xu W, et al. 2021. Immunity. 54(3):526-541.e7. PubMed
  27. Sudan R, et al. 2022. Front Immunol. 13:830961. PubMed
  28. Schnoegl D, et al. 2022. Front Immunol. 13:909270. PubMed
  29. Maier B, et al. 2020. Nature. 580:257. PubMed
  30. An Y et al. 2018. Cell reports. 25(3):702-714 . PubMed
  31. Kästele V, et al. 2021. Mucosal Immunol. 14:717. PubMed
  32. Sutiwisesak R, et al. 2020. PLoS Pathog. 16:e1009000. PubMed
RRID
AB_1937287 (BioLegend Cat. No. 135002)

Antigen Details

Structure
Type I transmembrane glycoprotein, associate with CD132, 60-90 kD
Distribution

Immature B cells through early pre-B stage, thymocytes (except CD4/CD8 double positive thymocytes), peripheral T cells, bone marrow stromal cells

Function
T cell and immature B cell proliferation and development
Ligand/Receptor
IL-7
Cell Type
B cells, T cells, Thymocytes
Biology Area
Immunology
Molecular Family
CD Molecules, Cytokine/Chemokine Receptors
Antigen References

1. Sudo T, et al. 1993. P. Natl. Acad. Sci. USA 90:9125.
2. Okuno Y, et al. 2001. P. Natl. Acad. Sci. USA 99:6246.
3. Pillai M, et al. 2004. Leukemia Lymphoma 45:2403.

Gene ID
16197 View all products for this Gene ID
UniProt
View information about CD127 on UniProt.org

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Go To Top Version: 3    Revision Date: 01/15/2021

For Research Use Only. Not for diagnostic or therapeutic use.

 

This product is supplied subject to the terms and conditions, including the limited license, located at www.biolegend.com/terms) ("Terms") and may be used only as provided in the Terms. Without limiting the foregoing, BioLegend products may not be used for any Commercial Purpose as defined in the Terms, resold in any form, used in manufacturing, or reverse engineered, sequenced, or otherwise studied or used to learn its design or composition without express written approval of BioLegend. Regardless of the information given in this document, user is solely responsible for determining any license requirements necessary for user’s intended use and assumes all risk and liability arising from use of the product. BioLegend is not responsible for patent infringement or any other risks or liabilities whatsoever resulting from the use of its products.

 

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