Purified anti-mouse CD127 (IL-7Rα) Recombinant Antibody, QA17A28

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Clone: QA17A28 (See other available formats)
Regulatory Status: RUO
Other Names: IL-7 receptor α chain, IL-7Rα
Isotype: Mouse IgG1, κ
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Product Citations: publications

QA17A28_PURE_CD127_IL-7Ralpha_Antibody_RECOM_901020.png — C57BL/6 mouse splenocytes were stained with purified anti-mouse CD127 (IL-7Rα) recombinant (clone QA17A28) (left) or purified mouse IgG1a, κ isotype control (right) then followed by rat anti-mouse IgG1 PE. Cells were co-stained with CD3 APC.

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159902

500 µg

315€

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Description

CD127 is a 60-90 kD type I transmembrane glycoprotein also known as IL-7 receptor α chain or IL-7Rα. It forms a heterodimer with the common γ chain (γc or CD132) which is shared with the receptors for IL-2, IL-4, IL-9, IL-13, IL-15, and IL-21. CD127 is expressed on immature B cells through early pre-B stage, thymocytes (except CD4/CD8 double positive thymocytes), peripheral T cells, and bone marrow stromal cells. CD127 has been reported to be an useful marker for identifying memory and effector T cells. The ligation of IL-7 with its receptor is important for stimulation of mature and immature T cells as well as immature B cells proliferation and development.

Product Details

Technical Data Sheet (pdf)

Product Details

Verified Reactivity: Mouse
Antibody Type: Recombinant
Host Species: Mouse
Immunogen: Recombinant mouse IL-7R α Fc fusion protein
Formulation: Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide
Preparation: The antibody was purified by affinity chromatography.
Concentration: 0.5 mg/mL
Storage & Handling: The antibody solution should be stored undiluted between 2°C and 8°C.
Application: FC - Quality tested
Recommended Usage: Each lot of this antibody is quality control tested by immunofluorescent staining with flow cytometric analysis. For flow cytometric staining, the suggested use of this reagent is ≤ 0.125 µg per million cells in 100 µL volume. It is recommended that the reagent be titrated for optimal performance for each application.
Application Notes: Clone QA17A28 is able to completely block the binding of clone A7R34 to IL-7Rα.
RRID: AB_2876561 (BioLegend Cat. No. 159902)

Antigen Details

Structure: Type I transmembrane glycoprotein, 60-90 kD
Distribution: Immature B cells through early pre-B stage, thymocytes (except CD4/CD8 double positive thymocytes), peripheral T cells, bone marrow stromal cells
Function: T cell and immature B cell proliferation and development
Interaction: CD132
Ligand/Receptor: IL-7
Antigen References: 1. Sudo T, et al. 1993. P. Natl. Acad. Sci. USA 90:9125.
2. Okuno Y, et al. 2001. P. Natl. Acad. Sci. USA 99:6246.
3. Pillai M, et al. 2004. Leukemia Lymphoma 45:2403
Gene ID: 16197 View all products for this Gene ID
UniProt: View information about CD127 on UniProt.org

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Cell Surface Flow Cytometry Staining Protocol

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Other Formats

View All CD127 Reagents Request Custom Conjugation

Description	Clone	Applications
Purified anti-mouse CD127 (IL-7Rα) Recombinant Antibody	QA17A28	FC
APC anti-mouse CD127 (IL-7Rα) Recombinant Antibody	QA17A28	FC
FITC anti-mouse CD127 (IL-7Rα) Recombinant Antibody	QA17A28	FC
PE anti-mouse CD127 (IL-7Rα) Recombinant Antibody	QA17A28	FC

Certificate of Analysis

Go To Top Version: 1 Revision Date: 09/10/2020

For Research Use Only. Not for diagnostic or therapeutic use.

This product is supplied subject to the terms and conditions, including the limited license, located at www.biolegend.com/terms) ("Terms") and may be used only as provided in the Terms. Without limiting the foregoing, BioLegend products may not be used for any Commercial Purpose as defined in the Terms, resold in any form, used in manufacturing, or reverse engineered, sequenced, or otherwise studied or used to learn its design or composition without express written approval of BioLegend. Regardless of the information given in this document, user is solely responsible for determining any license requirements necessary for user’s intended use and assumes all risk and liability arising from use of the product. BioLegend is not responsible for patent infringement or any other risks or liabilities whatsoever resulting from the use of its products.

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