Purified anti-mouse CD120a (TNF R Type I/p55) Antibody

Pricing & Availability
Clone
55R-286 (See other available formats)
Other Names
TNFR type I, TNFRI, TNFR60, p55, TNFRSF1A
Isotype
Armenian Hamster IgG
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Product Citations
publications
55R-286_Purified_083107
NIH/3T3 cell line stained with purified 55R-286, followed by biotinylated anti-Armenian hamster IgG and Sav-PE
  • 55R-286_Purified_083107
    NIH/3T3 cell line stained with purified 55R-286, followed by biotinylated anti-Armenian hamster IgG and Sav-PE
Cat # Size Price Quantity Avail. Save
113001 50 µg 56€
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Description

CD120a is a 55 kD Type I transmembrane protein, also known as Tumor Necrosis Factor Receptor Type I (TNFRI) or p55. It is expressed on a variety of cells at low levels. Resting T cells and erythrocytes express very little to no CD120a. This receptor binds both TNF-α and LT-α (also known as TNF-β). In association with TRADD and RIP, the receptor crosslinking induced by TNF-α or LT-α trimers is critical for signal transduction, leading to apoptosis, NF-kB activation, increased expression of proinflammatory genes, tumor necrosis, and cell differentiation depending on cell type and differentiation state.

Product Details
Technical Data Sheet (pdf)

Product Details

Reactivity
Mouse
Antibody Type
Monoclonal
Host Species
Armenian Hamster
Immunogen
E. coli-expressed extracellular domain of the mouse TNFRI protein
Formulation
Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide.
Preparation
The antibody was purified by affinity chromatography.
Concentration
0.5 mg/ml
Storage & Handling
The antibody solution should be stored undiluted between 2°C and 8°C.
Application

FC - Quality tested
IP, ELISA Capture, WB - Reported in the literature

Recommended Usage

Each lot of this antibody is quality control tested by immunofluorescent staining with flow cytometric analysis. For flow cytometric staining, the suggested use of this reagent is ≤ 1.0 µg per 106 cells in 100 µl volume. It is recommended that the reagent be titrated for optimal performance for each application.

Application Notes

Flow Cytometry3,4: For most successful immunofluorescent staining results, it may be important to maximize signal over background by using a relatively bright fluorochrome-antibody conjugate (Cat. No. 113003/113004)or by using a high sensitivity, three-layer staining technique (e.g., including a biotinylated anti-hamster IgG second step (Cat. No. 405501), followed by SAv-PE (Cat. No. 405204)).
ELISA Capture2: The 55R-286 antibody is useful as a capture antibody when paired with the biotinylated 55R-170 antibody (Cat. No. 112904) as the detecting antibody.
Additional reported applications (for the relevant formats) include: Western blotting and immunoprecipitation1.

Application References

(PubMed link indicates BioLegend citation)
  1. Pinckard, J.K., et al. 1997. J. Biol Chem. 272:10784. (IP)
  2. Sheehan, K.C., et al. 1995. J. Exp. Med. 181:607. (ELISA Capture)
  3. Schlaepfer, D.D., et al. 2007. J. Biol. Chem. 282:17450. (FC)
  4. Bell, J.H., et al. 2007. J. Leukocyte Biol. 82:173. (FC)
  5. Kuroishi, T., et al. 2008. J. Leukocyte Biol. 83:912.
Product Citations
  1. Kuroishi T, et al. 2008. J Leukoc Biol. 83:912. PubMed
Publication Library
RRID
AB_313530 (BioLegend Cat. No. 113001)

Antigen Details

Structure
TNFR superfamily, 55 kD
Distribution

Variety of cell types at low levels

Function
Apoptosis, NF-κB activation, inflammation, tumor necrosis, cell differentiation
Ligand/Receptor
TNF-α, LT-α (TNF-β)
Biology Area
Immunology, Innate Immunity
Molecular Family
CD Molecules, Cytokine/Chemokine Receptors
Antigen References

1. Aggarwal, B. B., et al. 1985. Nature 318 665.
2. Chan, F. K.M., et al. 2000. Science 288:2351.
3. Loetscher, H., et al. 1990. Cell 61:351.
4. Rothe, J., et al. 1993. Nature 364:798.

Gene ID
21937 View all products for this Gene ID
UniProt
View information about CD120a on UniProt.org

Related FAQs

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Go To Top Version: 2    Revision Date: 09/25/2014

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