Purified anti-MLKL Antibody

Pricing & Availability
Clone
W17104A (See other available formats)
Other Names
MLKL, HMLKL
Isotype
Rat IgG2a, κ
Ave. Rating
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Product Citations
publications
W17104A_Purified_MLKL_Antibody_040219
Western blot analysis of 15µg cell lysates from human PC-3, HaCaT, HeLa (Positive), Daudi (Negative) and Raw264.7 (Mouse) cells. Samples were resolved by electrophoresis (4-12% Bis-Tris gel), transferred to nitrocellulose, probed with 1 µg/mL anti-MLKL antibody, clone W17104A, followed by incubation with horseradish peroxidase (HRP) labeled goat anti-rat secondary antibody (Cat. No. 405405, 1:3000 dilution). Direct-Blot™ HRP anti-GAPDH (Cat. No. 607904, 1:10000 dilution). Western-Ready™ ECL Substrate Kit (Cat. No. 426303) was used for developing.
  • W17104A_Purified_MLKL_Antibody_040219
    Western blot analysis of 15µg cell lysates from human PC-3, HaCaT, HeLa (Positive), Daudi (Negative) and Raw264.7 (Mouse) cells. Samples were resolved by electrophoresis (4-12% Bis-Tris gel), transferred to nitrocellulose, probed with 1 µg/mL anti-MLKL antibody, clone W17104A, followed by incubation with horseradish peroxidase (HRP) labeled goat anti-rat secondary antibody (Cat. No. 405405, 1:3000 dilution). Direct-Blot™ HRP anti-GAPDH (Cat. No. 607904, 1:10000 dilution). Western-Ready™ ECL Substrate Kit (Cat. No. 426303) was used for developing.
Cat # Size Price Quantity Avail. Save
666801 25 µg 68€
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666802 100 µg 172€
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Description

MLKL has a four-helix bundle (4HB) domain at the N-terminal and a C-terminal PsKD connected via a two-helix linker. MLKL is important in the necroptotic signaling pathways. During the process, phosphorylated RIP3 (receptor-interacting protein 3) is recruited and phosphorylates MLKL which can induce the oligomerization of MLKL. Oligomerized MLKL translates to the plasma membrane, destroying the membrane integrity and inducing necroptosis. Knockdown or inhibition of MLKL prohibits necrosis. High expression of MLKL and RIP3 are reported in inflammatory bowel disease. MLKL is also involved in endosomal trafficking and extracellular vesical generation, which is necroptosis independent.

Product Details
Technical Data Sheet (pdf)

Product Details

Reactivity
Human
Antibody Type
Monoclonal
Host Species
Rat
Immunogen
Human recombinant MLKL Protein (1-193 a.a.) expressed in E. Coli.
Formulation
Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide.
Preparation
The antibody was purified by affinity chromatography.
Concentration
0.5 mg/ml
Storage & Handling
The antibody solution should be stored undiluted between 2°C and 8°C.
Application

WB - Quality tested

Recommended Usage

Each lot of this antibody is quality control tested by Western blotting. For Western blotting, the suggested use of this reagent is 0.25 - 2.5 µg per ml (1:200 - 1:2000 dilution). It is recommended that the reagent be titrated for optimal performance for each application.

RRID
AB_2801124 (BioLegend Cat. No. 666801)
AB_2801125 (BioLegend Cat. No. 666802)

Antigen Details

Distribution

Cytoplasm

Function
Necroptosis
Interaction
RIP3
Biology Area
Apoptosis/Tumor Suppressors/Cell Death, Cell Biology, Cell Death, Signal Transduction
Molecular Family
Protein Kinases/Phosphatase
Gene ID
197259 View all products for this Gene ID
UniProt
View information about MLKL on UniProt.org

Related FAQs

There are no FAQs for this product.
Go To Top Version: 1    Revision Date: 04/02/2019

For research use only. Not for diagnostic use. Not for resale. BioLegend will not be held responsible for patent infringement or other violations that may occur with the use of our products.

 

*These products may be covered by one or more Limited Use Label Licenses (see the BioLegend Catalog or our website, www.biolegend.com/ordering#license). BioLegend products may not be transferred to third parties, resold, modified for resale, or used to manufacture commercial products, reverse engineer functionally similar materials, or to provide a service to third parties without written approval of BioLegend. By use of these products you accept the terms and conditions of all applicable Limited Use Label Licenses. Unless otherwise indicated, these products are for research use only and are not intended for human or animal diagnostic, therapeutic or commercial use.

 

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Toll-Free Phone: 1-877-Bio-Legend (246-5343) Phone: (858) 768-5800 Fax: (877) 455-9587

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