Purified anti-MAP2 Antibody (Previously Covance catalog# PCK-554P)

Pricing & Availability
Poly28225 (See other available formats)
Regulatory Status
Other Names
Microtubule-associated protein 2, MAP-2
Covance Catalog# PCK-554P
Chicken IgY
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Product Citations
Immunohistochemical staining of MAP2 Chicken Polyclonal Antibody, on formalin fixed paraffin embedded mouse brain tissue at 1/10,000 dilution.
  • Poly28225_Purified_MAP2_Antibody_IHC_032615
    Immunohistochemical staining of MAP2 Chicken Polyclonal Antibody, on formalin fixed paraffin embedded mouse brain tissue at 1/10,000 dilution.
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822501 50 µL 300€
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Microtubules are 25nm diameter protein rods found in most kinds of eukaryote cells. They are polymerized from a dimeric subunit made of one α subunit and one β tubulin subunit. Microtubules are associated with a family of proteins called microtubule associated proteins (MAPs), which includes the protein τ (tau) and a group of proteins referred to as MAP1, MAP2, MAP3, MAP4 and MAP5. MAP2 is made up of two ~280kD apparent molecular weight bands referred to as MAP2a and MAP2b. A third lower molecular weight form, usually called MAP2c, corresponds to a pair of protein bands running at ~70 kD on SDS-PAGE gels. All these MAP2 forms are derived from a single gene by alternative transcription, and all share a C-terminal sequence which includes either three or four microtubule binding peptide sequences, which are very similar to those found in the related microtubule binding protein τ (tau). MAP2 isoforms are expressed only in neuronal cells and specifically in the perikarya and dendrites of these cells. Antibodies to MAP2 are therefore excellent markers on neuronal cells, their perikarya and neuronal dendrites. In contrast τ (tau) is found predominantly in neuronal axons.

Product Details
Technical Data Sheet (pdf)

Product Details

Verified Reactivity
Human, Rat, Mouse, Bovine
Antibody Type
Host Species
This antibody was made against MAP2 in chickens.
The antibody was purified by affinity chromatography.
Lot-specific (to obtain lot-specific concentration, please enter the lot number in our Concentration and Expiration Lookup or Certificate of Analysis online tools.)
Storage & Handling
The antibody solution should be stored undiluted between 2°C and 8°C. Please note the storage condition for this antibody has been changed from -20°C to between 2°C and 8°C. You can also check your vial or your CoA to find the most accurate storage condition for this antibody.

IHC-P - Quality tested

Recommended Usage

Each lot of this antibody is quality control tested by formalin-fixed paraffin-embedded immunohistochemical staining.

The optimal working dilution should be determined for each specific assay condition.
WB: 1:100,000*
IF: 1:10,000

Application Notes

This antibody is effective in immunoblotting (WB) and immunofluorescence (IF). This antibody does not bind to protein A or protein G.

*Predicted MW = 280 kD and a doublet ~70 kD

Application References

(PubMed link indicates BioLegend citation)
  1. Savas JN, et al. 2010. J Biol Chem. 285:13142-53. [IF] PubMed
Product Citations
  1. Bartoll A, et al. 2020. EMBO Mol Med. 12:e11776. PubMed
  2. Kodani Y, et al. 2022. Front Endocrinol (Lausanne). 13:941166. PubMed
  3. Lin YT et al. 2018. Neuron. 98(6):1141-1154 . PubMed
  4. Bresciani A, et al. 2018. PLoS One. 9:306. PubMed
  5. van Ineveld RL, et al. 2022. Nat Protoc. 17:3028. PubMed
  6. Tang QY, et al. 2020. Stem Cell Reports. 0.929861111. PubMed
  7. Ka M, Moffat J, Kim W 2016. Cereb Cortex. 10.1093/cercor/bhw319. PubMed
  8. Raja WK, et al. 2022. PLoS One. 17:e0277532. PubMed
  9. Cui H, et al. 2022. STAR Protoc. 3:101813. PubMed
  10. Savas J, et al. 2010. J Biol Chem. 285:13142-13153. PubMed
  11. Sepehrimanesh M, et al. 2021. STAR Protoc. 2:100917. PubMed
  12. Fischer M, et al. 2021. Alcohol Clin Exp Res. 45:979. PubMed
  13. Merlini M et al. 2019. Neuron. 101(6):1099-1108 . PubMed
  14. Gomez-Sintes R, et al. 2022. Nat Commun. 13:4220. PubMed
  15. Castagnola S, et al. 2020. Genome Res. 1633:30. PubMed
  16. Almansa D, et al. 2022. Int J Mol Sci. 23:. PubMed
  17. Sathler MF, et al. 2021. J Cell Sci. 134:. PubMed
AB_2564858 (BioLegend Cat. No. 822501)

Antigen Details

Cell Type
Neural Stem Cells
Biology Area
Cell Biology, Neuroscience, Neuroscience Cell Markers, Stem Cells
Molecular Family
Gene ID
4133 View all products for this Gene ID
View information about MAP2 on UniProt.org

Related FAQs

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Go To Top Version: 7    Revision Date: 01/07/2021

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  • Purified anti-MAP2


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