Purified anti-human MIF Antibody

Pricing & Availability
Clone
10C3 (See other available formats)
Other Names
Macrophage migration inhibitory factor (MMIF), Glycosylation inhibiting factor (GIF), GLIF
Isotype
Mouse IgG2b, κ
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Product Citations
publications
MIF_Human_Antibody_ELISA_020514
  • MIF_Human_Antibody_ELISA_020514
  • n10C3_Purified_MIF_Antibody_WB_051314.jpg
    Western blot analysis of THP-1 (lane 1), Raw264.7(lane 2), PC-3(lane 3) and Jurkat(lane 4) cells using anti-MIF antibody (10C3). GAPDH antibody (poly6314) was used as loading control.
Cat # Size Price Quantity Avail. Save
525501 50 µg 84€
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525502 500 µg 244€
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Description

Human macrophage migration inhibitory factor (MIF) is a 12.5 kD, 115 amino acid, non-glycosylated polypeptide expressed by multiple cell types, including activated T cells, macrophages, eosinophils, epithelial cells, and endothelial cells. MIF plays many roles in biological processes such as catalytic activity, immunity, endocrine regulation, signal modulation, and inflammation.

MIF is expressed in malignant cells including lung, liver, breast, colon, and prostate tumors. Studies suggest that MIF might serve as a molecular link between chronic inflammation and cancer.

Recombinant MIF consists of a mixture of monomers, dimers, and trimers. The physiologically active forms are believed to be predominantly dimeric and trimeric forms.

Product Details
Technical Data Sheet (pdf)

Product Details

Reactivity
Human
Antibody Type
Monoclonal
Host Species
Mouse
Immunogen
Recombinant human MIF with a His tag
Formulation
Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide.
Preparation
The antibody was purified by affinity chromatography.
Concentration
0.5 mg/ml
Storage & Handling
The antibody solution should be stored undiluted between 2°C and 8°C.
Application

ELISA Capture - Quality tested
WB - Validated

Recommended Usage

Each lot of this protein is quality control tested by ELISA assay.  A concentration of 4 µg/ml of the capture antibody was utilized to generate the example standard curve. It is recommended that each lot of reagent be titrated for optimal performance for each application.

Application Notes

ELISA: To measure human MIF, the purified 10C3 antibody (Cat. No. 525501) is useful as the capture antibody in a sandwich ELISA assay, when used in conjunction with the biotinylated 10C3 antibody (Cat. No. 525503) as the detection antibody and Recombinant Human MIF (ELISA Std.) (Cat. No. 594609) as the standard.

Additional reported applications (for the relevant formats) include: functional assay1 and neutralization1. The LEAF™ purified antibody (Endotoxin <0.1 EU/µg, Azide-Free, 0.2 µm filtered) is recommended for functional assays (contact our custom solutions team).

Note: For testing human MIF in serum, plasma or cell culture supernatant, BioLegend's LEGEND MAX™ ELISA Kits (Cat. No. 438407 & 438408) are specially developed and recommended.

Application References

(PubMed link indicates BioLegend citation)
  1. Zhou H, et al. 2009. PLoS ONE. 4:e6087. (FA, Neut)
RRID
AB_2563133 (BioLegend Cat. No. 525501)
AB_2563134 (BioLegend Cat. No. 525502)

Antigen Details

Structure
Predominantly a homotrimer
Distribution

Activated T-cells, macrophages, eosinophils, epithelial cells, and endothelial cells

Function
Pro-inflammatory cytokine, regulates macrophage function, involved in cancer development, tautomerase activity
Interaction
Monocytes and macrophages, mesenchymal cells, B-cells
Ligand/Receptor
CD74, CXCR4, CXCR2, CXCR7
Cell Type
T cells, Macrophages, Eosinophils, Epithelial cells, Endothelial cells
Biology Area
Angiogenesis, Apoptosis/Tumor Suppressors/Cell Death, Cell Biology, Cell Cycle/DNA Replication, Immunology, Innate Immunity, Signal Transduction
Molecular Family
Cytokines/Chemokines
Antigen References

1. Zhou H, et al. 2009. PLoS ONE. 4:e6087.
2. Potolicchio I, et al. 2003. J. Biol. Chem. 278:30889.
3. Meyer-Siegler K & Iczkowski K. 2005. BMC Cancer. 5:73.
4. Babu SN, et al. 2012. Asian Pac. J. Cancer Prev. 13:1737.

Gene ID
4282 View all products for this Gene ID
UniProt
View information about MIF on UniProt.org

Related FAQs

There are no FAQs for this product.
Go To Top Version: 2    Revision Date: 05/13/2014

For research use only. Not for diagnostic use. Not for resale. BioLegend will not be held responsible for patent infringement or other violations that may occur with the use of our products.

 

*These products may be covered by one or more Limited Use Label Licenses (see the BioLegend Catalog or our website, www.biolegend.com/ordering#license). BioLegend products may not be transferred to third parties, resold, modified for resale, or used to manufacture commercial products, reverse engineer functionally similar materials, or to provide a service to third parties without written approval of BioLegend. By use of these products you accept the terms and conditions of all applicable Limited Use Label Licenses. Unless otherwise indicated, these products are for research use only and are not intended for human or animal diagnostic, therapeutic or commercial use.

 

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Your actual data may vary due to variations in samples, target cells, instruments and their settings, staining conditions, and other factors.
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