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Purified anti-human CD4 Antibody

Pricing & Availability
Clone
A17070D (See other available formats)
Regulatory Status
RUO
Other Names
T4/Leu-3
Isotype
Mouse IgG2b, κ
Ave. Rating
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Product Citations
publications
A17070D_PURE_CD4_Antibody_111020
Human paraffin-embedded tonsil tissue slices were prepared with a standard protocol of deparaffinization and rehydration. Antigen retrieval was done with Sodium Citrate H.I.E.R. 1X at 95°C for 40 minutes. Tissue was washed with PBS/0.05% Tween 20 twice for five minutes and blocked with 5% FBS and 0.2% gelatin for 30 minutes. Then, the tissue was stained with 10 µg/mL purified anti-human CD4 (clone A17070D) at 4°C overnight, followed by 2.5 µg/mL anti-mouse IgG (Poly4053) Alexa Fluor® 594 (red) for two hours at room temperature. Nuclei were counterstained with DAPI (green). The image was captured with a 10X objective.
  • A17070D_PURE_CD4_Antibody_111020
    Human paraffin-embedded tonsil tissue slices were prepared with a standard protocol of deparaffinization and rehydration. Antigen retrieval was done with Sodium Citrate H.I.E.R. 1X at 95°C for 40 minutes. Tissue was washed with PBS/0.05% Tween 20 twice for five minutes and blocked with 5% FBS and 0.2% gelatin for 30 minutes. Then, the tissue was stained with 10 µg/mL purified anti-human CD4 (clone A17070D) at 4°C overnight, followed by 2.5 µg/mL anti-mouse IgG (Poly4053) Alexa Fluor® 594 (red) for two hours at room temperature. Nuclei were counterstained with DAPI (green). The image was captured with a 10X objective.
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396202 100 µg 141€
 
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Description

CD4, also known as T4/Leu-3, is a 55 kD single-chain type I transmembrane glycoprotein and member of the immunoglobulin superfamily. It is expressed on most thymocytes, helper T cells, type II NKT cells, and monocytes/macrophages. CD4 is part of the TCR/CD3 complex, binds to β2 domain from the MHC class II molecule, and participates in TCR signal transduction. CD4 is the receptor of IL-16 and is a coreceptor for the human immunodeficiency virus (HIV) and human herpes virus 7 (HHV-7).

 

Product Details
Technical Data Sheet (pdf)

Product Details

Verified Reactivity
Human
Antibody Type
Monoclonal
Host Species
Mouse
Immunogen
Recombinant human CD4
Formulation
Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide
Preparation
The antibody was purified by affinity chromatography.
Concentration
0.5 mg/mL
Storage & Handling
The antibody solution should be stored undiluted between 2°C and 8°C.
Application

IHC-P - Quality tested
Recommended Usage

Each lot of this antibody is quality control tested by formalin-fixed paraffin-embedded immunohistochemical staining. For immunohistochemistry, a concentration range of 5 - 10 µg/mL is suggested. It is recommended that the reagent be titrated for optimal performance for each application.

RRID
AB_2888738 (BioLegend Cat. No. 396202)

Antigen Details

Structure
55 kD, type I transmembrane glycoprotein, member of the Ig superfamily
Distribution

Helper T cells, subset of thymocytes, monocytes/macrophages, type II NKT cells

Interaction
Part of the TCR/CD3 complex
Ligand/Receptor
β2 domain from the MHC class II
Antigen References

1. Zhu J, et al. 2010. Annu Rev. Immunol. 28:445.
2. Vignali DA. 2010. J. Immunol. 184:5933.
3. Zhou L, et al. 2009. Immunity 30:646.
4. Singer A, et al. 2008. Nat. Rev. Immunol. 8:788.
5. Zhu J and Paul WE. 2008. Blood 112:1557.

Gene ID
920 View all products for this Gene ID
UniProt
View information about CD4 on UniProt.org

Related FAQs

There are no FAQs for this product.
Go To Top Version: 1    Revision Date: 11/10/2020

For research use only. Not for diagnostic use. Not for resale. BioLegend will not be held responsible for patent infringement or other violations that may occur with the use of our products.

 

*These products may be covered by one or more Limited Use Label Licenses (see the BioLegend Catalog or our website, www.biolegend.com/ordering#license). BioLegend products may not be transferred to third parties, resold, modified for resale, or used to manufacture commercial products, reverse engineer functionally similar materials, or to provide a service to third parties without written approval of BioLegend. By use of these products you accept the terms and conditions of all applicable Limited Use Label Licenses. Unless otherwise indicated, these products are for research use only and are not intended for human or animal diagnostic, therapeutic or commercial use.

 

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