- 5-271 (See other available formats)
- Regulatory Status
- Other Names
- GPIIIb, GPIV
- Mouse IgG2a, κ
- Ave. Rating
- Submit a Review
- Product Citations
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CD36 is an 85 kD integral membrane glycoprotein, also known as GPIIIb, or GPIV. It is expressed on various epithelial and endothelial cells as well as erythrocytes, platelets, macrophages/monocytes and some macrophage-derived dendritic cells. CD36 functions as a scavenger receptor, binding thrombospondin, long chain fatty acids, oxidized LDL, collagen type I, IV, and V as well as apoptotic cells. The 5-271 antibody has been reported to be useful for flow cytometry.Product Details
- Antibody Type
- Host Species
- Human platelets
- Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide and EDTA.
- The antibody was purified by affinity chromatography.
- 1.0 mg/ml
- Storage & Handling
- The antibody solution should be stored undiluted between 2°C and 8°C.
FC - Quality tested
CyTOF® - Verified
- Recommended Usage
This product is suitable for use with the Maxpar® Metal Labeling Kits. The product is formulated to simplify the antibody preparation when performing the labeling protocol. As a result, it is possible to proceed directly to the step to Partially Reduce the Antibody by adding 100 µl of Maxpar® Ready antibody to 100 µl of 4 mM TCEP-R in a 50 kDa filter and continue with the protocol. Always refer to the latest version of Maxpar® User Guide when conjugating Maxpar® Ready antibodies.
- Application Notes
Additional reported applications (for the relevant formats) include: immunofluorescence2.
- Additional Product Notes
Maxpar® is a registered trademark of Fluidigm Inc.
(PubMed link indicates BioLegend citation)
- Stelner E, et al. 2006. J. Cell Sci. 119:459.
- Stewart DA, et al. 2012. Mol. Cancer Res. 10:727. (IF)
- Product Citations
AB_2563745 (BioLegend Cat. No. 336215)
- Integral membrane protein, CD36 family, single chain glycoprotein, 85-113 kD
Expressed in various epithelial and endothelial cells, erythrocytes, platelets, monocytes/ macrophages, some macrophage-derived dendritic cells
- Scavenger receptor for thrombospondin, long chain fatty acids, anionic phospholipids, oxidized LDL, collagen types I, IV and V. Involved in recognition and phagocytosis of apoptotic cells, cell adhesion.
- Thrombospondin, anionic phospholipids, oxidized LDL, collage types I, IV, and V
- Cell Type
- Dendritic cells, Endothelial cells, Epithelial cells, Erythrocytes, Macrophages, Monocytes, Platelets
- Biology Area
- Cell Biology, Immunology, Innate Immunity, Neuroinflammation, Neuroscience
- Molecular Family
- CD Molecules
- Antigen References
1. Hogg N, et al. 1984. Immunology 53-753.
2. Greenwalt DE, et al. 1992. Blood 80:1105.
3. Armsesilla AL, et al.1994. J. Biol. Chem. 269:18985.
4. Endemann G, et al. 1993. J. Biol. Chem. 268:11811.
- Gene ID
- 948 View all products for this Gene ID
- View information about CD36 on UniProt.org
- Can I obtain CyTOF data related to your Maxpar® Ready antibody clones?
We do not test our antibodies by mass cytometry or on a CyTOF machine in-house. The data displayed on our website is provided by Fluidigm®. Please contact Fluidigm® directly for additional data and further details.
- Can I use Maxpar® Ready format clones for flow cytometry staining?
We have not tested the Maxpar® Ready antibodies formulated in solution containing EDTA for flow cytometry staining. While it is likely that this will work in majority of the situations, it is best to use the non-EDTA formulated version of the same clone for flow cytometry testing. The presence of EDTA in some situations might negatively affect staining.
- I am having difficulty observing a signal after conjugating a metal tag to your Maxpar® antibody. Please help troubleshoot.
We only supply the antibody and not test that in house. Please contact Fluidigm® directly for troubleshooting advice: http://techsupport.fluidigm.com/
- Is there a difference between buffer formulations related to Maxpar® Ready and purified format antibodies?
The Maxpar® Ready format antibody clones are formulated in Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide and EDTA. The regular purified format clones are formulated in solution that does not contain any EDTA. Both formulations are however without any extra carrier proteins.
|Purified anti-human CD36||5-271||FC, IHC-F, ICC|
|APC anti-human CD36||5-271||FC|
|APC/Cyanine7 anti-human CD36||5-271||FC|
|FITC anti-human CD36||5-271||FC|
|PE anti-human CD36||5-271||FC|
|Purified anti-human CD36 (Maxpar® Ready)||5-271||FC, CyTOF®|
|Biotin anti-human CD36||5-271||FC|
|APC/Fire™ 750 anti-human CD36||5-271||FC|
|PE/Cyanine7 anti-human CD36||5-271||FC|
|PerCP/Cyanine5.5 anti-human CD36||5-271||FC|
|TotalSeq™-A0407 anti-human CD36||5-271||PG|
|TotalSeq™-C0407 anti-human CD36||5-271||PG|
|Brilliant Violet 421™ anti-human CD36||5-271||FC|
|Alexa Fluor® 488 anti-human CD36||5-271||FC|
|TotalSeq™-B0407 anti-human CD36||5-271||PG|
|Alexa Fluor® 700 anti-human CD36 Antibody||5-271||FC|
|TotalSeq™-D0407 anti-human CD36||5-271||PG|
Compare Data Across All Formats
This data display is provided for general comparisons between formats.
Your actual data may vary due to variations in samples, target cells, instruments and their settings, staining conditions, and other factors.
If you need assistance with selecting the best format contact our expert technical support team.
Purified anti-human CD36
APC anti-human CD36
APC/Cyanine7 anti-human CD36
FITC anti-human CD36
PE anti-human CD36
Purified anti-human CD36 (Maxpar® Ready)
Biotin anti-human CD36
APC/Fire™ 750 anti-human CD36
PE/Cyanine7 anti-human CD36
PerCP/Cyanine5.5 anti-human CD36
TotalSeq™-A0407 anti-human CD36
TotalSeq™-C0407 anti-human CD36
Brilliant Violet 421™ anti-human CD36
Alexa Fluor® 488 anti-human CD36
TotalSeq™-B0407 anti-human CD36
Alexa Fluor® 700 anti-human CD36 Antibody
TotalSeq™-D0407 anti-human CD36