Purified anti-human CD231 (TALLA) Antibody

Pricing & Availability
Clone
SN1a (M3-3D9) (See other available formats)
Regulatory Status
RUO
Workshop
VII 70397
Other Names
kT-cell acute lymphoblastic leuemia antigen, TALLA-1, transmembrane 4 superfamily, TM4SF2
Isotype
Mouse IgG1, κ
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Product Citations
publications
SN1a_Pure_042408.jpg
Human T-lymphoblastic leukemia cell line Jurkat stained with purified SN1a (M3-3D9), followed by anti-mouse IgG FITC
  • SN1a_Pure_042408.jpg
    Human T-lymphoblastic leukemia cell line Jurkat stained with purified SN1a (M3-3D9), followed by anti-mouse IgG FITC
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329402 100 µg 95€
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Description

CD231 is 150 kD (28-45 kD reduced) cell surface glycoprotein belonging to the Transmembrane 4 Superfamily (TM4SF also known as tetraspanins). It was originally described as T-cell Acute Lymphoblastic Leukemia Antigen (TALLA or TALLA-1). CD231 is expressed on T-cell acute lymphoblastic leukemia, neuroblastoma cells, skeletal muscle, spleen and normal brain neuron. CD231 plays a role in integrin-mediated migration, however, its biological function is not fully clear yet.

Product Details
Technical Data Sheet (pdf)

Product Details

Verified Reactivity
Human
Reported Reactivity
African Green, Baboon, Cynomolgus
Antibody Type
Monoclonal
Host Species
Mouse
Immunogen
isolated human CD231 antigen
Formulation
Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide.
Preparation
The antibody was purified by affinity chromatography.
Concentration
0.5 mg/ml
Storage & Handling
The antibody solution should be stored undiluted between 2°C and 8°C.
Application

FC - Quality tested
IP, IHC-F, ICC - Reported in the literature, not verified in house

Recommended Usage

Each lot of this antibody is quality control tested by immunofluorescent staining with flow cytometric analysis. For flow cytometric staining, the suggested use of this reagent is ≤ 2.0 µg per 106 cells in 100 µl volume or 100 µl of whole blood. It is recommended that the reagent be titrated for optimal performance for each application.

Application Notes

Additional reported applications (for the relevant formats) include: immunoprecipitation1, immunohistochemical staining of acetone-fixed frozen tissue sections1, immunofluorescence2

Application References

(PubMed link indicates BioLegend citation)
  1. Matsuzaki H and Seon BK. 1987. Cancer Res. 47:4283.
  2. Seon BK, et al. 1983. Proc. Natl. Acad. Sci. USA. 80:845.
RRID
AB_2213454 (BioLegend Cat. No. 329402)

Antigen Details

Distribution

T-cell acute lymphoblastic leukemia, skeletal muscle, spleen, neuroblastoma cells and normal brain neuron

Cell Type
Leukemia, Neurons
Biology Area
Immunology
Molecular Family
CD Molecules
Antigen References

1. Matsuzaki H and Seon BK. 1987. Cancer Res. 47:4283.
2. Seon BK, et al. 1983. Proc. Natl. Acad. Sci. USA. 80:845.
3. Hara H and Seon BK. 1986. Proc. Natl. Acad. Sci. USA 84:3390.

Gene ID
7102 View all products for this Gene ID
UniProt
View information about CD231 on UniProt.org

Related FAQs

There are no FAQs for this product.
Go To Top Version: 2    Revision Date: 09/01/2022

For Research Use Only. Not for diagnostic or therapeutic use.

 

This product is supplied subject to the terms and conditions, including the limited license, located at www.biolegend.com/terms) ("Terms") and may be used only as provided in the Terms. Without limiting the foregoing, BioLegend products may not be used for any Commercial Purpose as defined in the Terms, resold in any form, used in manufacturing, or reverse engineered, sequenced, or otherwise studied or used to learn its design or composition without express written approval of BioLegend. Regardless of the information given in this document, user is solely responsible for determining any license requirements necessary for user’s intended use and assumes all risk and liability arising from use of the product. BioLegend is not responsible for patent infringement or any other risks or liabilities whatsoever resulting from the use of its products.

 

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This data display is provided for general comparisons between formats.
Your actual data may vary due to variations in samples, target cells, instruments and their settings, staining conditions, and other factors.
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