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Purified anti-human CD223 (LAG-3) Antibody

Pricing & Availability
Clone
11C3C65 (See other available formats)
Regulatory Status
RUO
Other Names
CD223, LAG-3, LAG3, lymphocyte-activation gene-3
Isotype
Mouse IgG1, κ
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Product Citations
publications
11C3C65_PURE_CD223_Antibody_1_05062016
PHA-stimulated (3-day) human peripheral blood lymphocytes were stained with CD3 APC/Cy7 and purified human CD223 (LAG-3, clone 11C3C65) (top) or mouse IgG1, κ isotype control (bottom), followed by anti-mouse IgG1 PE.
  • 11C3C65_PURE_CD223_Antibody_1_05062016
    PHA-stimulated (3-day) human peripheral blood lymphocytes were stained with CD3 APC/Cy7 and purified human CD223 (LAG-3, clone 11C3C65) (top) or mouse IgG1, κ isotype control (bottom), followed by anti-mouse IgG1 PE.
  • 11C3C65_PURE_CD223_Antibody_2_05062016
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369302 100 µg 231€
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Description

CD223, also known as LAG-3, is a 70 kD type I transmembrane glycoprotein that is involved in T-cell signaling. Similar to CD4, CD223 binds MHC class II, but with a higher affinity. CD223 negatively regulates T-cell activation. It is expressed by activated T-cells and natural killer cells (NKs), as well as regulatory T-cells. It is transiently expressed on the surface of activated T-cells in acute conditions but high expression is maintained under tolerizing conditions. CD223 deficiency results in reduced tumor growth. CD223 and PD-1 can act in synergy and reverse exhausted phenotypes, improve tumor rejection, and control viral load.

Product Details
Technical Data Sheet (pdf)

Product Details

Verified Reactivity
Human
Antibody Type
Monoclonal
Host Species
Mouse
Immunogen
Human LAG-3 transfected cells.
Formulation
Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide.
Preparation
The antibody was purified by affinity chromatography.
Concentration
0.5 mg/ml
Storage & Handling
The antibody solution should be stored undiluted between 2°C and 8°C.
Application

FC - Quality tested
Recommended Usage

Each lot of this antibody is quality control tested by immunofluorescent staining with flow cytometric analysis. For flow cytometric staining, the suggested use of this reagent is ≤0.5 µg per million cells in 100 µl volume. It is recommended that the reagent be titrated for optimal performance for each application.

Application Notes

The staining of clone 11C3C65 cannot be blocked by clone 7H2C65, which is another anti-human CD223 (LAG-3) antibody.

Product Citations
  1. Gide TN, et al. 2019. Cancer Cell. 35:238. PubMed
  2. Tian Y, et al. 2019. Cell Rep. 29:4482. PubMed
  3. Liu F, et al. 2020. J Immunother Cancer. 8:. PubMed
  4. O'Boyle KC, et al. 2020. Methods Mol Biol. 2111:1. PubMed
  5. Cobb DA, et al. 2022. J Immunother Cancer. 10:. PubMed
  6. Schulte–Schrepping J, et al. 2020. Cell. 182(6):1419-1440. PubMed
  7. Macmillan ML, et al. 2021. Blood Adv. 5:1425. PubMed
  8. Georg P, et al. 2022. Cell. 185:493. PubMed
RRID
AB_2616876 (BioLegend Cat. No. 369302)

Antigen Details

Structure
70 kD transmembrane glycoprotein, Ig superfamily, highly homologous to CD4.
Distribution

Activated T-cells and natural killer cells (NKs) and regulatory T cells.

Function
Negatively regulates T-cell activation.
Ligand/Receptor
Binds MHC class II molecules.
Cell Type
Dendritic cells, NK cells, T cells, Tregs
Biology Area
Immunology, Inhibitory Molecules
Molecular Family
CD Molecules, Immune Checkpoint Receptors
Antigen References

1. Castelli C, et al. 2014. Oncoimmunology. 3(11):e967146.
2. Poirier N, et al. 2011. Clin. Exp. Immunol. 164:265.
3. Juno JA, et al. 2015. Retrovirology. 12:17.
4. Casati C, et al. 2006. Cancer Res. 66:4450.

Gene ID
3902 View all products for this Gene ID
UniProt
View information about CD223 on UniProt.org

Related FAQs

There are no FAQs for this product.
Go To Top Version: 0    Revision Date: 05/18/2016

For research use only. Not for diagnostic use. Not for resale. BioLegend will not be held responsible for patent infringement or other violations that may occur with the use of our products.

 

*These products may be covered by one or more Limited Use Label Licenses (see the BioLegend Catalog or our website, www.biolegend.com/ordering#license). BioLegend products may not be transferred to third parties, resold, modified for resale, or used to manufacture commercial products, reverse engineer functionally similar materials, or to provide a service to third parties without written approval of BioLegend. By use of these products you accept the terms and conditions of all applicable Limited Use Label Licenses. Unless otherwise indicated, these products are for research use only and are not intended for human or animal diagnostic, therapeutic or commercial use.

 

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This data display is provided for general comparisons between formats.
Your actual data may vary due to variations in samples, target cells, instruments and their settings, staining conditions, and other factors.
If you need assistance with selecting the best format contact our expert technical support team.

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