- 1B7 (See other available formats)
- Regulatory Status
- Other Names
- RNA binding protein fox-1 homolog 3, fox-1 homolog C, neuronal nuclei, hexaribonucleotide binding protein 3
Covance Catalog# SIG-39860
- Mouse IgG2b, κ
- Ave. Rating
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- Product Citations
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Fox3 is one of a family of 3 mammalian Fox homologues. Fox was discovered in C. elegans as a gene involved in sex determination, and the name Fox is an acronym of "Feminizing locus on X". The Fox protein and its 3 mammalian homologues are all about 46 kD proteins, each of which includes a central highly conserved RRM type RNA recognition motif, which corresponds to a small ~70 amino acid structure consisting of 4 beta strands and two alpha-helices. Fox3 is a protein which has a function in RNA splicing and is expressed heavily and specifically in neuronal nuclei. The 1B7 antibody reveals strong nuclear and distal cytoplasmic staining for Fox3/NeuN and the complete absence of staining of astrocytes and other kinds of non-neuronal cells. This Fox3/NeuN antibody is therefore an excellent marker of neuronal cells.Product Details
- Human, Mouse, Rat
- Antibody Type
- Host Species
- Antibody was raised against the N-terminal 100 amino acids of human Fox3 as expressed in and purified from E. coli.
- Phosphate-buffered solution + 10mM sodium azide.
- The antibody was purified by affinity chromatography.
- 1 mg/mL
- Storage & Handling
- The antibody solution should be stored undiluted between 2°C and 8°C. Please note the storage condition for this antibody has been changed from -20°C to between 2°C and 8°C. You can also check your vial or your CoA to find the most accurate storage condition for this antibody.
IHC-P - Quality tested
WB - Verified
ICC, IHC-F - Reported in the literature, not verified in house
- Recommended Usage
Each lot of this antibody is quality control tested by formalin-fixed paraffin-embedded immunohistochemical staining. For immunohistochemistry, a concentration range of 0.5 - 1.0 µg/mL is suggested. For western blotting, the suggested use of this reagent is 0.01 - 0.05 µg/mL. It is recommended that the reagent be titrated for optimal performance for each application.
- Application Notes
The double band observed in the immunoblot corresponds to the two NeuN isoforms produced by alternative splicing with expected molecular weights of 46 kD and 48 kD.
(PubMed link indicates BioLegend citation)
- Herculano-Houzel S and Lent R. 2005. J Neurosci. 25:2518-21. (ICC)
- Kim KK, et al. 2009. J Biol Chem. 284:31052-31061. (WB, ICC, IHC-P)
- Underwood JG, et al. 2005. Mol Cell Biol. 25:10005-10016. (IHC-F, WB)
- Product Citations
AB_2734601 (BioLegend Cat. No. 834502)
AB_2564991 (BioLegend Cat. No. 834501)
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