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Purified anti-DDX58 (RIG-I) Antibody

Pricing & Availability
Clone
9F1B20 (See other available formats)
Regulatory Status
RUO
Other Names
DEAD box protein 58, RIG-I-like receptor 1 (RLR-1), Retinoic acid-inducible gene 1 protein (RIG-1)
Isotype
Mouse IgG2a, κ
Ave. Rating
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Product Citations
publications
9F1B20_Purified_DDX58_Antibody_040419_updated.png
Total cell lysates (15 µg total protein) from undifferentiated THP-1 cells (-) and THP-1 cells treated with PMA for 3 days followed by an overnight LPS treatment (+) were resolved by 4-12% Bis-Tris gel electrophoresis, transferred to a nitrocellulose membrane, and probed with 2.0 µg/mL (1:250 dilution) of Purified anti-DDX58 (RIG-1) Antibody, clone 9F1B20, overnight at 4°C. Proteins were visualized by chemiluminescence detection using HRP goat anti-mouse IgG Antibody (Cat. No. 405306) at a 1:3000 dilution. Direct-Blot™ HRP anti-β-Actin Antibody (Cat. No. 664804) was used as a loading control at a 1:25000 dilution (lower). Lane M: Molecular Weight marker.
  • 9F1B20_Purified_DDX58_Antibody_040419_updated.png
    Total cell lysates (15 µg total protein) from undifferentiated THP-1 cells (-) and THP-1 cells treated with PMA for 3 days followed by an overnight LPS treatment (+) were resolved by 4-12% Bis-Tris gel electrophoresis, transferred to a nitrocellulose membrane, and probed with 2.0 µg/mL (1:250 dilution) of Purified anti-DDX58 (RIG-1) Antibody, clone 9F1B20, overnight at 4°C. Proteins were visualized by chemiluminescence detection using HRP goat anti-mouse IgG Antibody (Cat. No. 405306) at a 1:3000 dilution. Direct-Blot™ HRP anti-β-Actin Antibody (Cat. No. 664804) was used as a loading control at a 1:25000 dilution (lower). Lane M: Molecular Weight marker.
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662302 100 µg 231€
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Description

DDX58, also named RIG-1, is a member of the DExD/H-box-containing RNA helicase family. DDX58 was first reported as a retinoic acid-inducible gene and subsequently characterized as a pattern recognition receptor (PRR). DDX58 is responsible for sensing cytosolic viral RNA and has been shown to initiate innate antiviral immune responses. DDX58 is composed of two caspase recruitment domains (CARDs), a helicase domain, and a C-terminal regulatory domain (CTD). In response to virus infections, the DDX58 CTD domain binds 5'-triphosphorylated dsRNA, which induces multimerization of DDX58 with the adaptor molecule MAVS on the mitochondria through the CARD domains. MAVS transduces the signal to activate downstream transcription factors, such as IRF-3, IRF-7, and NF-κB, leading to production of proinflammatory cytokines and type I interferons.

Product Details
Technical Data Sheet (pdf)

Product Details

Verified Reactivity
Human
Antibody Type
Monoclonal
Host Species
Mouse
Immunogen
Partial DDX58 recombinant protein (1-228 a.a.) expressed in E. coli.
Formulation
Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide.
Preparation
The antibody was purified by affinity chromatography.
Concentration
0.5 mg/ml
Storage & Handling
The antibody solution should be stored undiluted between 2°C and 8°C.
Application

WB - Quality tested
Recommended Usage

Each lot of this antibody is quality control tested by Western blotting. For Western blotting, the suggested use of this reagent is 0.5 - 2.5 µg per ml. It is recommended that the reagent be titrated for optimal performance for each application.

RRID
AB_2564246 (BioLegend Cat. No. 662302)

Antigen Details

Structure
925 amino acids with a predicted molecular weight of 107 kD. Contains two CARD domains, one RNA helicase domain and one CTD domain.
Distribution

Cytoplasm

Function
DDX58 is a RNA helicase participating in the innate immune responses during viral infection. It acts as a cytoplasmic sensor of viral RNA and activates the production of proinflammatory cytokines and type I interferons.
Interaction
DDX58 forms homodimers upon binding to viral double stranded (ds) RNA. Interacts with TMEM173, DHX58, TRIM25, NLRC5, and MAVS.
Biology Area
Cell Biology, Immunology, Innate Immunity, Signal Transduction
Antigen References

1. Goubau D, et al. 2013. Immunity. 38:855.
2. Zhang HX, et al. 2013. Proc. Natl. Acad. Sci. USA. 110:6459.
3. Wang Y, et al. 2013. Innate Immun. 19:193.
4. Oshiumi H, et al. 2012. J. Biochem. 151:5.
5. Kolakofsky D, et al. 2012. RNA. 18:2118.
6. Schlee M, et al. 2010. Mol. Ther. 18:1254.

Gene ID
23586 View all products for this Gene ID
UniProt
View information about DDX58 on UniProt.org

Related FAQs

There are no FAQs for this product.
Go To Top Version: 3    Revision Date: 04/04/2019

For research use only. Not for diagnostic use. Not for resale. BioLegend will not be held responsible for patent infringement or other violations that may occur with the use of our products.

 

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