- C-11 (See other available formats)
- Regulatory Status
- Other Names
- Cytokeratin, Keratin
- Mouse IgG1, κ
- Ave. Rating
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- Product Citations
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Cytokeratins are intermediate filament proteins that are widely expressed in many tissues. These proteins are vital components of the cytoskeleton and interact with a number of other proteins. Cytokeratins are usually found in the cytoplasm,although some cytokeratins can be found in the nucleus and the nucleolus.Product Details
- Verified Reactivity
- Antibody Type
- Host Species
- Keratin enriched fraction from human epidermoid carcinoma.
- This antibody is provided in phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide at 0.5 mg/ml.
- The antibody was purified by affinity chromatography.
- 0.5 mg/ml
- Storage & Handling
- The antibody solution should be stored undiluted between 2°C and 8°C.
WB - Quality tested
ICC - Verified
IP, IHC-P - Reported in the literature, not verified in house
- Recommended Usage
Each lot of this antibody is quality control tested by Western blotting. Western blotting, suggested working dilution(s): Use 5 µg antibody per 5 ml antibody dilution buffer for each mini-gel. For immunocytochemistry, a concentration range of 1.0 - 4.0 μg/ml is recommended. It is recommended that the reagent be titrated for optimal performance for each application.
- Application Notes
The C-11 monoclonal antibody reacts with a conserved epitope of human cytokeratin 4, 5, 6, 8, 10, 13, and 18. This antibody has been shown to be useful for Western blotting and has also been reported to be useful for immunoprecipitation, immunohistochemistry (paraffin sections), immunocytochemistry, and spatial biology (IBEX)7,8.
(PubMed link indicates BioLegend citation)
- Kovarik J. 1988. Int. J. Cancer Suppl. 3:50.
- Bartek J, et al. 1991. J. Pathol. 164:215.
- Chernyavsky A I, 2007. J. Biol. Chem. doi:10.1074/jbc.M611365200. PubMed
- Comito G, et al. 2012. Cancer Lett. 324:31. PubMed
- Vojt-òsek B, et al. 1989. Folia Biol (Praha). 35:373. (reactivity with rat, dog, sheep, pig, cow)
- Dekaney CM, et al. 2005. Gastroenterology 129:1567. (ICC)
- Radtke AJ, et al. 2020. Proc Natl Acad Sci U S A. 117:33455-65. (SB) PubMed
- Radtke AJ, et al. 2022. Nat Protoc. 17:378-401. (SB) PubMed
- Product Citations
AB_439774 (BioLegend Cat. No. 628601)
AB_439775 (BioLegend Cat. No. 628602)
- Intermediate filament protein, contains three coiled-coil domains. Cytokeratins exist as heterotetramers composed of two type I and two type II keratin subunits
Cytokeratins are widely expressed proteins usually found in the cytoplasm although some cytokeratins can also be found in the nucleus and the nucleolus
- Intermediate filament protein involved with the cytoskeleton.
- Most cytokeratins interact with a number of other proteins such as 14-3-3, EGF receptor, and many others
- Biology Area
- Cell Biology, Cell Motility/Cytoskeleton/Structure, Neuroscience, Neuroscience Cell Markers
- Molecular Family
- Intermediate Filaments
- Gene ID
- 3875 View all products for this Gene ID
- View information about Cytokeratin pan reactive on UniProt.org
|Purified anti-Cytokeratin (pan reactive)||C-11||WB, ICC, IP, IHC-P|
|Alexa Fluor® 647 anti-Cytokeratin (pan reactive)||C-11||ICC, SB, ICFC|
|Alexa Fluor® 594 anti-Cytokeratin (pan reactive)||C-11||ICC|
|Alexa Fluor® 488 anti-Cytokeratin (pan reactive)||C-11||ICC, IHC-P|
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