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Purified anti-CD324 (E-Cadherin) Antibody

Pricing & Availability
Clone
4A2 (See other available formats)
Regulatory Status
RUO
Other Names
Cadherin-1, CAM 120/80, Epithelial cadherin, Uvomorulin, CD_antigen, CD324 Antigen, CDHE, ECAD, LCAM
Isotype
Mouse IgG1, κ
Ave. Rating
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Product Citations
publications
4A2_PURE_CD324_Antibody_082719
Western blot of purified anti-CD324 (E-Cadherin) antibody (clone 4A2). Lane 1: Molecular weight marker; Lane 2: 30 µg of human skin lysate; Lane 3: 30 µg of rat colon lysate. The blot was incubated with 5 µg/mL of the primary antibody overnight at 4°C, followed by incubation with HRP-labeled goat anti-mouse IgG (Cat. No. 405306). Enhanced chemiluminescence was used as the detection system. The top arrow indicates the full length E-Cadherin protein. The bottom arrow indicates the 33-kDa C-terminal fragment (CTF2).
  • 4A2_PURE_CD324_Antibody_082719
    Western blot of purified anti-CD324 (E-Cadherin) antibody (clone 4A2). Lane 1: Molecular weight marker; Lane 2: 30 µg of human skin lysate; Lane 3: 30 µg of rat colon lysate. The blot was incubated with 5 µg/mL of the primary antibody overnight at 4°C, followed by incubation with HRP-labeled goat anti-mouse IgG (Cat. No. 405306). Enhanced chemiluminescence was used as the detection system. The top arrow indicates the full length E-Cadherin protein. The bottom arrow indicates the 33-kDa C-terminal fragment (CTF2).
Cat # Size Price Quantity Check Availability Save
866701 25 µg 85€
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866702 100 µg 221€
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Description

E-cadherin is a calcium-dependent cell adhesion molecule (CAM) critical for formation and functions of adherent junctions during development and tumorigenesis. E-cadherin is usually expressed and localized at the surface of epithelial cells. However, it is also expressed in the adult and embryonic forebrain germinal zones in vivo and in cells derived from these regions growing in vitro. Conditional loss of E-cadherin in mouse brain causes defects in the self-renewal of neural stem cells both in vivo and in vitro. Overexpression of E-cadherin increases the number of neural stem cell colonies indicating that E-cadherin is required for the neural stem cell self-renewal function. In human glioblastoma tissue, the expression of E-cadherin is increased and usually correlates with unfavorable outcome.

Product Details
Technical Data Sheet (pdf)

Product Details

Verified Reactivity
Human, Rat
Antibody Type
Monoclonal
Host Species
Mouse
Formulation
Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide.
Preparation
The antibody was purified by affinity chromatography.
Concentration
0.5 mg/ml
Storage & Handling
The antibody solution should be stored undiluted between 2°C and 8°C.
Application

WB - Quality tested
Recommended Usage

Each lot of this antibody is quality control tested by Western blotting. For Western blotting, the suggested use of this reagent is 1.0 - 10 µg per ml. It is recommended that the reagent be titrated for optimal performance for each application.

It is also recommended using EDTA-based solutions for dissociating attachment-dependent cell lines. 

Application Notes

Lower MW bands detected in WB are cleaved C terminal fragments.

Cleavage of E-cadherin usually occurs in tumor development and cleaved E-cadherin fragments of ~29-38 kD often possess distinct oncogenic properties.

Application References

(PubMed link indicates BioLegend citation)
  1. David, J.M. and Rajasekaran, A.K. 2012. Cancer Res. 72(12): 2917–2923.
Product Citations
  1. Lin CY, et al. 2022. Int J Mol Sci. 23:. PubMed
RRID
AB_2814610 (BioLegend Cat. No. 866701)
AB_2814611 (BioLegend Cat. No. 866702)

Antigen Details

Structure
Human CD324 (E-cadherin) is a 882 amino acid protein with a predicted molecular mass of 97 kD and observed molecular mass of ~120 kD.
Distribution

Tissue distribution: Non-neural epithelial tissues and forebrain germinal zones
Cellular distribution: Golgi, plasma membrane, endosome, and cell

Function
CD324 (E-cadherin) is involved in cell adhesion.
Interaction
Interacts with catenin family members
Cell Type
Embryonic Stem Cells, Epithelial cells, Neural Stem Cells
Biology Area
Cell Adhesion, Cell Biology, Immuno-Oncology, Immunology, Neuroscience, Stem Cells
Molecular Family
Adhesion Molecules, CD Molecules
Antigen References
  1. Lewis-Tuffin LJ, et al. 2010. PLoS One. 5(10):e13665.
  2. Karpowicz P, et al. 2009. J Neurosci. 29(12):3885-96.
Gene ID
999 View all products for this Gene ID
UniProt
View information about CD324 on UniProt.org

Related FAQs

There are no FAQs for this product.
Go To Top Version: 2    Revision Date: 08/27/2019

For research use only. Not for diagnostic use. Not for resale. BioLegend will not be held responsible for patent infringement or other violations that may occur with the use of our products.

 

*These products may be covered by one or more Limited Use Label Licenses (see the BioLegend Catalog or our website, www.biolegend.com/ordering#license). BioLegend products may not be transferred to third parties, resold, modified for resale, or used to manufacture commercial products, reverse engineer functionally similar materials, or to provide a service to third parties without written approval of BioLegend. By use of these products you accept the terms and conditions of all applicable Limited Use Label Licenses. Unless otherwise indicated, these products are for research use only and are not intended for human or animal diagnostic, therapeutic or commercial use.

 

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This data display is provided for general comparisons between formats.
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  • Purified anti-CD324 (E-Cadherin)
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