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Purified anti-Caspase-3 Antibody

Pricing & Availability
Clone
W20054B (See other available formats)
Regulatory Status
RUO
Other Names
Caspase 3 preproprotein, Casp3, cpp32, Apopain, Yama, PARP cleavage protease, SREBP cleavage activity 1
Isotype
Rat IgG2a, κ
Ave. Rating
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Product Citations
publications
W20054B_PURE_Caspase-3_Antibody_120121
Total cell lysates (15 µg total protein) from Jurkat (Lane 1, untreated control) and Jurkat treated with 1 µM of staurosporine (Lane 2) for 3 hours were resolved by 4-12 % Bis-Tris gel electrophoresis, transferred to a nitrocellulose membrane, and probed with 0.5 μg/mL of anti-Caspase-3 antibody overnight at 4°C. Proteins were visualized by chemiluminescence detection using HRP goat anti-rat IgG antibody (Cat. No. 405405) at 1:3000 dilution. Direct-Blot™ HRP anti-β actin antibody (Cat. No. 643807) was used as a loading control at a 1:10,000 dilution (lower). Western-Ready™ ECL Substrate Premium Kit (Cat. No. 426319) was used as a detection reagent. Lane M: Molecular weight ladder
  • W20054B_PURE_Caspase-3_Antibody_120121
    Total cell lysates (15 µg total protein) from Jurkat (Lane 1, untreated control) and Jurkat treated with 1 µM of staurosporine (Lane 2) for 3 hours were resolved by 4-12 % Bis-Tris gel electrophoresis, transferred to a nitrocellulose membrane, and probed with 0.5 μg/mL of anti-Caspase-3 antibody overnight at 4°C. Proteins were visualized by chemiluminescence detection using HRP goat anti-rat IgG antibody (Cat. No. 405405) at 1:3000 dilution. Direct-Blot™ HRP anti-β actin antibody (Cat. No. 643807) was used as a loading control at a 1:10,000 dilution (lower). Western-Ready™ ECL Substrate Premium Kit (Cat. No. 426319) was used as a detection reagent. Lane M: Molecular weight ladder
Cat # Size Price Quantity Check Availability Save
949801 25 µg 95€
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949802 100 µg 235€
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Description

Caspase 3 is a member of the peptidase family C14. This cytoplasmic caspase functions as a heterotetramer and has two isoforms (one acts as a dominant negative inhibitor). The pro-form of this caspase is approximately 32 kD. Caspase 3 is involved in the caspase activation cascade responsible for apoptosis execution. Caspase 3 is inhibited by c-IAP1, c-IAP2, XIAP, survivin, and Livin and activated by Caspase 6, -8, and -10, granzyme B, Apaf-1 by cleavage into large p17 and small p12 subunits. Caspase 3 has been shown to cleave PARP, huntingtin, androgen receptor, atrophin-1, DNA- PKcs, D4-GDI, hnRNPs C1/C2, U1snRNP, PKC delta, ATM, fodrin, gelsolin, ICAD, RIP, X-IAP, STAT1, topoisomerase I, vimentin, Rb, lamin B, mdm2, and β-catenin. 

Product Details
Technical Data Sheet (pdf)

Product Details

Verified Reactivity
Human
Antibody Type
Monoclonal
Host Species
Rat
Immunogen
Partial recombinant human Caspase-3 protein
Formulation
Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide
Preparation
The antibody was purified by affinity chromatography.
Concentration
0.5 mg/mL
Storage & Handling
The antibody solution should be stored undiluted between 2°C and 8°C.
Application

WB - Quality tested
Recommended Usage

Each lot of this antibody is quality control tested by western blotting. For western blotting, the suggested use of this reagent is 0.25 - 1.0 µg/mL. It is recommended that the reagent be titrated for optimal performance for each application.

Application Notes

This antibody recognizes endogenous human Caspase-3 proteins in both total and spliced forms from Jurkat cell lysates treated with or without staurosporine. 

RRID
AB_2904464 (BioLegend Cat. No. 949801)
AB_2904464 (BioLegend Cat. No. 949802)

Antigen Details

Structure
Caspase 3 is a 277 amino acid protein with a molecular mass of 32 kD.
Distribution

Nucleus and cytosol

Function
Caspase 3 regulates programmed cell death (apoptosis)
Interaction
Active heterodimers between large p17 subunit and small p11 subunit of Casp7 and vice versa. Cleaves PARP, huntingtin, androgen receptor, atrophin-1, DNA- PKcs, D4-GDI, hnRNPs C1/C2, U1snRNP, PKC delta, ATM, fodrin, gelsolin, ICAD, RIP, X-IAP, STAT1, topo
Cell Type
B cells, Endothelial cells, Fibroblasts, Monocytes, T cells
Biology Area
Apoptosis/Tumor Suppressors/Cell Death, Cell Biology, Cell Death, Neuroscience
Antigen References
  1. Goldberg YP, et al. 1996. Nat Genet. 13:442-9.
  2. Fernandes-Alnemri T, et al. 1996. Proc Natl Acad Sci U S A. 93:7464-9.
  3. Slee EA, et al. 2001. J Biol Chem. 276:7320-6.
  4. Woo M, et al. 2003. Nat Immunol. 4:1016-22.
Gene ID
836 View all products for this Gene ID
UniProt
View information about Caspase-3 on UniProt.org

Related FAQs

There are no FAQs for this product.
Go To Top Version: 1    Revision Date: 12/01/2021

For research use only. Not for diagnostic use. Not for resale. BioLegend will not be held responsible for patent infringement or other violations that may occur with the use of our products.

 

*These products may be covered by one or more Limited Use Label Licenses (see the BioLegend Catalog or our website, www.biolegend.com/ordering#license). BioLegend products may not be transferred to third parties, resold, modified for resale, or used to manufacture commercial products, reverse engineer functionally similar materials, or to provide a service to third parties without written approval of BioLegend. By use of these products you accept the terms and conditions of all applicable Limited Use Label Licenses. Unless otherwise indicated, these products are for research use only and are not intended for human or animal diagnostic, therapeutic or commercial use.

 

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  • Purified anti-Caspase-3
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