Purified anti-BAD Antibody

Pricing & Availability
Clone
W16055A (See other available formats)
Other Names
Bcl2-associated agonist of cell death, Bcl-2-binding component 6 (Bbc6), Bcl-xL/Bcl-2-associated death promoter, Bcl2 antagonist of cell death.
Isotype
Rat IgG2a, κ
Ave. Rating
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Product Citations
publications
W16055A_PURE_BAD_WB_022317
Total cell lysates (15 µg protein) from HeLa, A431, A20 and NIH3T3 were resolved by 4-12% Bis-tris gel electrophoresis, transferred to nitrocellulose, and probed with 5 µg/mL purified anti-BAD (clone W16055A) antibody. Proteins were visualized using an HRP goat anti-rat-IgG secondary antibody (clone Poly4054) and chemiluminescence detection. Direct-Blot™ HRP anti-β-actin (clone 2F1-1) antibody was used as a loading control.
  • W16055A_PURE_BAD_WB_022317
    Total cell lysates (15 µg protein) from HeLa, A431, A20 and NIH3T3 were resolved by 4-12% Bis-tris gel electrophoresis, transferred to nitrocellulose, and probed with 5 µg/mL purified anti-BAD (clone W16055A) antibody. Proteins were visualized using an HRP goat anti-rat-IgG secondary antibody (clone Poly4054) and chemiluminescence detection. Direct-Blot™ HRP anti-β-actin (clone 2F1-1) antibody was used as a loading control.
Cat # Size Price Quantity Avail. Save
695502 100 µg 188€
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Description

BCL-2 associated agonist of cell death (BAD) is a member of the BCL-2 family, which is involved in the regulation of the mitochondrial apoptosis pathway. The BCL-2 protein family members can either promote cell survival (BCL-2, BCL-XL, and MCL-1) or induce apoptosis (BAX, BAK, BIM, BID, and BAD). The BCL-2 family proteins form homodimers or heterodimers through the conserved BCL-2 homology motifs (BH1, BH2, BH3, and BH4). BAD belongs to the BH3-only proteins and functions as a direct antagonist of the prosurvival effect mediated by BCL-2 and BCL-XL. BAD is inactivated by several survival kinases, such as AKT, PKA, and RSK, through phosphorylation. Upon stimulation of survival signals, phosphorylated BAD dissociates from BCL-2/ BCL-XL complexes and is sequestered in the cytoplasm by 14-3-3 scaffold proteins. Without interacting with BAD, BCL-2 and BCL-XL are able to suppress apoptosis by preventing release of cytochrome c from mitochondria and blocking subsequent activation of caspases.

Product Details
Technical Data Sheet (pdf)

Product Details

Reactivity
Human, Mouse
Antibody Type
Monoclonal
Host Species
Rat
Immunogen
Full length mouse BAD recombinant protein expressed in E. coli.
Formulation
Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide.
Preparation
The antibody was purified by affinity chromatography.
Concentration
0.5 mg/ml
Storage & Handling
The antibody solution should be stored undiluted between 2°C and 8°C.
Application

WB - Quality tested

Recommended Usage

Each lot of this antibody is quality control tested by Western blotting. For Western blotting, the suggested use of this reagent is 1.0 - 5.0 µg per ml. It is recommended that the reagent be titrated for optimal performance for each application.

Product Citations
  1. Chea C, et al. 2018. PLoS One. 13:e0191683. PubMed
RRID
AB_2650854 (BioLegend Cat. No. 695502)

Antigen Details

Structure
204 amino acids with a predicted molecular weight of 22.1 kD. Contains a BH3 domain responsible for dimer formation.
Distribution

Mitochondrion outer membrane and cytoplasm.

Function
BAD is a proapoptotic protein, promoting cell apoptosis through inactivating the BCL-2 family members BCL-2 and BCL-XL.
Interaction
Forms homodimers or heterodimers with other members of the same family. Interacts with S100A10, 14-3-3, AKT1, PIM3, NOL3, and HIF3A.
Biology Area
Apoptosis/Tumor Suppressors/Cell Death, Cell Biology
Antigen References

1. Cekanova M, et al. 2015. Exp. Cell. Res. 331:1.
2. Pareja F, et al. 2014. Mol. Cancer Res. 12:987.
3. Sakamaki J, et al. 2011. Proc. Natl. Acad. Sci. USA 108:6085.
4. Yu C, et al. 2004. Mol. Cell. 13:329-40.
5. Cartier A, et al. 2003. Exp. Cell Res. 291:242.
6. Moriishi K, et al. 2002. Virology 292:258.

Gene ID
12015 View all products for this Gene ID
UniProt
View information about BAD on UniProt.org

Related FAQs

There are no FAQs for this product.
Go To Top Version: 0    Revision Date: 03/14/2017

For research use only. Not for diagnostic use. Not for resale. BioLegend will not be held responsible for patent infringement or other violations that may occur with the use of our products.

 

*These products may be covered by one or more Limited Use Label Licenses (see the BioLegend Catalog or our website, www.biolegend.com/ordering#license). BioLegend products may not be transferred to third parties, resold, modified for resale, or used to manufacture commercial products, reverse engineer functionally similar materials, or to provide a service to third parties without written approval of BioLegend. By use of these products you accept the terms and conditions of all applicable Limited Use Label Licenses. Unless otherwise indicated, these products are for research use only and are not intended for human or animal diagnostic, therapeutic or commercial use.

 

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