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PerCP/Cyanine5.5 anti-mouse CX3CR1 Antibody

Pricing & Availability
Clone
SA011F11 (See other available formats)
Regulatory Status
RUO
Other Names
Chemokine (C-X3-C motif) receptor 1, Fractalkine receptor, GPR13, CCRL1, CMKBRL1, CMKDR1, V28
Isotype
Mouse IgG2a, κ
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Product Citations
publications
SA011F11_PerCPCy5.5_CX3CR1_Antibody_FC_1_102714
C57BL/6 mouse splenocytes were stained with CD11b APC and CX3CR1 (clone SA011F11) PerCP/Cyanine5.5 (top) or mouse IgG2a, κ PerCP/Cyanine5.5 isotype control (bottom).
  • SA011F11_PerCPCy5.5_CX3CR1_Antibody_FC_1_102714
    C57BL/6 mouse splenocytes were stained with CD11b APC and CX3CR1 (clone SA011F11) PerCP/Cyanine5.5 (top) or mouse IgG2a, κ PerCP/Cyanine5.5 isotype control (bottom).
  • SA011F11_PerCPCy5.5_CX3CR1_Antibody_FC_2_102714
Compare all formats See PerCP/Cyanine5.5 spectral data
Cat # Size Price Quantity Check Availability Save
149009 25 µg 141€
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149010 100 µg 306€
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Description

CX3CR1 is a 40 kD, G-protein coupled receptor, with seven transmembrane regions. CX3CR1 is expressed by resident and alternatively activated macrophages (M2), a subset of monocytes, dendritic cells (DCs), NK cells, a subset of memory T cells, and mast cells. CX3CR1 is involved in cell recruitment during inflammation and participates in cell adhesion and extravasation from blood vessels. Its ligand is CX3CL1, also known as fractalkine or neurotactin. CX3CR1 is also a coreceptor for HIV1 and variations in this gene leads to increased susceptibility to HIV. In the brain, it is expressed by glial cells, which interact with CX3CL1 expressed by neurons.

Product Details
Technical Data Sheet (pdf)

Product Details

Verified Reactivity
Mouse
Antibody Type
Monoclonal
Host Species
Mouse
Immunogen
Mouse CX3CR1-transfected cells
Formulation
Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide; may contain stabilizer.
Preparation
The antibody was purified by affinity chromatography and conjugated with PerCP/Cyanine5.5 under optimal conditions.
Concentration
0.2 mg/ml
Storage & Handling
The antibody solution should be stored undiluted between 2°C and 8°C, and protected from prolonged exposure to light. Do not freeze.
Application

FC - Quality tested
Recommended Usage

Each lot of this antibody is quality control tested by immunofluorescent staining with flow cytometric analysis. For flow cytometric staining, the suggested use of this reagent is ≤0.06 µg per million cells in 100 µl volume. It is recommended that the reagent be titrated for optimal performance for each application.

* PerCP/Cyanine5.5 has a maximum absorption of 482 nm and a maximum emission of 690 nm.

Application Notes

View more applications data for a different format of this clone in our Scientific Poster Library.

Additional Product Notes

BioLegend is in the process of converting the name PerCP/Cy5.5 to PerCP/Cyanine5.5. The dye molecule remains the same, so you should expect the same quality and performance from our PerCP/Cyanine5.5 products. Contact Technical Service if you have any questions.

Product Citations
  1. Huang J, et al. 2021. Immunity. 54(4):673-686.e4. PubMed
  2. Corsi G, et al. 2021. Glia. 70:89. PubMed
  3. Bhattacherjee A, et al. 2019. Commun Biol. 2:450. PubMed
  4. Bhattacherjee A, et al. 2021. Mol Neurodegener. 16:19. PubMed
  5. He W et al. 2018. Immunity. 49(6):1175-1190 . PubMed
  6. Hulsmans M et al. 2017. Cell. 169(3):510-522 . PubMed
  7. Nahrendorf W, et al. 2021. eLife. 10:00. PubMed
  8. Martin MD, et al. 2020. Cell Rep. 31:107508. PubMed
  9. Youshani AS, et al. 2019. J Neuroinflammation. 16:25. PubMed
  10. Riopel M, et al. 2018. J Clin Invest. 128:1458. PubMed
  11. Boyce M, et al. 2022. Cells. 11:. PubMed
  12. Bantug GR, et al. 2018. Immunity. 48:542. PubMed
  13. van de Wouw M, et al. 2020. Microbiome. 8:67. PubMed
  14. Goldberg MF et al. 2018. Immunity. 49(6):1090-1102 . PubMed
RRID
AB_2564493 (BioLegend Cat. No. 149009)
AB_2564494 (BioLegend Cat. No. 149010)

Antigen Details

Structure
G-protein coupled receptor, 7 transmembrane regions, 40 kD.
Distribution

Resident and alternatively activated macrophages (M2), subset of monocytes, dendritic cells (DCs), natural killer cells (NK), subset of memory T cells, mast cells, glial cells, and astrocytes.

Function
Involved in cell recruitment during inflammation, participates in cell adhesion and extravasation from blood vessels, coreceptor for HIV1.
Ligand/Receptor
CX3CL1 (fractalkine, neurotactin).
Cell Type
Astrocytes, Dendritic cells, Macrophages, Mast cells, Microglia, Monocytes, NK cells, T cells
Biology Area
Cell Adhesion, Cell Biology, Immunology, Innate Immunity, Neuroscience, Neuroscience Cell Markers
Molecular Family
Cytokine/Chemokine Receptors, GPCR
Antigen References

1. Ponzetta A, et al. 2013. J. Immunol. 191:5684.
2. Jacquelin S, et al. 2013. Blood. 122:674.
3. Garcia JA, et al. 2013. J. Immunol. 191:1063.
4. Lee YS, et al. 2013. Cell. 153:413.
5. Shechter R, et al. 2013. Immunity. 38:555.

Gene ID
13051 View all products for this Gene ID
UniProt
View information about CX3CR1 on UniProt.org

Related FAQs

How stable is PerCP/Cyanine5.5 tandem as compared to PerCP alone?

PerCP/Cyanine5.5 is quite photostable and also better than PerCP alone in withstanding fixation.

Go To Top Version: 3    Revision Date: 05/05/2020

For research use only. Not for diagnostic use. Not for resale. BioLegend will not be held responsible for patent infringement or other violations that may occur with the use of our products.

 

*These products may be covered by one or more Limited Use Label Licenses (see the BioLegend Catalog or our website, www.biolegend.com/ordering#license). BioLegend products may not be transferred to third parties, resold, modified for resale, or used to manufacture commercial products, reverse engineer functionally similar materials, or to provide a service to third parties without written approval of BioLegend. By use of these products you accept the terms and conditions of all applicable Limited Use Label Licenses. Unless otherwise indicated, these products are for research use only and are not intended for human or animal diagnostic, therapeutic or commercial use.

 

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This data display is provided for general comparisons between formats.
Your actual data may vary due to variations in samples, target cells, instruments and their settings, staining conditions, and other factors.
If you need assistance with selecting the best format contact our expert technical support team.

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