- 1D3/CD19 (See other available formats)
- Regulatory Status
- Other Names
- B4, CVID3
- Rat IgG2a, κ
- Ave. Rating
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- Product Citations
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CD19 is a 95 kD glycoprotein also known as B4. It is a member of the Ig superfamily, expressed on all pro-B to mature B cells (during development) and follicular dendritic cells. Plasma cells do not express CD19. CD19, in association with CD21 and CD81, forms a molecular complex integral to B cell activation.Product Details
- Antibody Type
- Host Species
- Transfected k562 cells expressing the extracellular domain of murine CD19.
- Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide.
- The antibody was purified by affinity chromatography and conjugated with PerCP/Cyanine5.5 under optimal conditions.
- 0.2 mg/ml
- Storage & Handling
- The antibody solution should be stored undiluted between 2°C and 8°C, and protected from prolonged exposure to light. Do not freeze.
FC - Quality tested
- Recommended Usage
Each lot of this antibody is quality control tested by immunofluorescent staining with flow cytometric analysis. For flow cytometric staining, the suggested use of this reagent is ≤0.25 µg per million cells in 100 µl volume. It is recommended that the reagent be titrated for optimal performance for each application.
* PerCP/Cyanine5.5 has a maximum absorption of 482 nm and a maximum emission of 690 nm.
- Application Notes
Additional reported applications (for the relevant formats) include: blocking1,2 and immunoprecipitation1.
- Additional Product Notes
- BioLegend is in the process of converting the name PerCP/Cy5.5 to PerCP/Cyanine5.5. The dye molecule remains the same, so you should expect the same quality and performance from our PerCP/Cyanine5.5 products. Contact Technical Service if you have any questions.
(PubMed link indicates BioLegend citation)
- Krop I, et al. 1996. Eur. J. Immunol. 26:238. (FC, Blocking, IP)
- Abraham P, et al. 2014. Clin. Exp. Immunol. 175:181. (Blocking)
- Product Citations
AB_2629814 (BioLegend Cat. No. 152405)
AB_2629815 (BioLegend Cat. No. 152406)
- Ig superfamily, associates with CD21 and CD81, 95 kD.
Pro-B cells to mature B cells (during development), follicular dendritic cells.
- Modulates B cell activation and differentiation.
- CD21, CD81, Leu-13.
- Cell Type
- B cells, Dendritic cells
- Biology Area
- Immunology, Costimulatory Molecules
- Molecular Family
- CD Molecules
- Antigen References
1. Fearon DT. 1993. Curr. Opin. Immunol. 5:341.
2. Krop I, et al. 1996. Eur. J. Immunol. 26:238.
3. Krop I, et al. 1996. J. Immunol. 157:48.
4. Tedder TF, et al. 1994. Immunol. Today 15:437.
- Gene ID
- 12478 View all products for this Gene ID
- View information about CD19 on UniProt.org
- How stable is PerCP/Cyanine5.5 tandem as compared to PerCP alone?
PerCP/Cyanine5.5 is quite photostable and also better than PerCP alone in withstanding fixation.
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