- APA5 (See other available formats)
- Regulatory Status
- Other Names
- PDGF receptor-α, PDGFR-α
- Rat IgG2a, κ
- Ave. Rating
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- Product Citations
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Platelet-derived growth factor receptor-α (PDGFR-α), CD140a, is one of two receptors for platelet-derived growth factors (PDGFs) and binds to all isoforms of PDGFs: PDGF-AA, PDGF-AB, and PDGF-BB. PDGFRa is a receptor tyrosine kinase that forms homodimers or heterodimers on the surface upon ligand binding and phosphorylates substrates. PDGFRs consist of either homodimers of α/α, β/β, or heterodimers of α/β. PDGF receptors, α and β, are single glycoproteins with intracellular tyrosine kinase domain. Their ligand, PDGF, is a mitogen for connective tissue and glial cells. CD140a is expressed on embryonic tissues and mesenchymal-derived cells of adult mice. PDGF plays a role in wound healing and acts as a chemoattractant for fibroblasts, smooth muscle cells, glial cells, monocytes, and neutrophils.Product Details
- Verified Reactivity
- Antibody Type
- Host Species
- Mouse PDGFR-α-hIgG1 recombinant fusion protein
- Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide.
- The antibody was purified by affinity chromatography and conjugated with PerCP/Cyanine5.5 under optimal conditions.
- 0.2 mg/ml
- Storage & Handling
- The antibody solution should be stored undiluted between 2°C and 8°C, and protected from prolonged exposure to light. Do not freeze.
FC - Quality tested
- Recommended Usage
Each lot of this antibody is quality control tested by immunofluorescent staining with flow cytometric analysis. For flow cytometric staining, the suggested use of this reagent is =1.5 µg per million cells in 100 µl volume. It is recommended that the reagent be titrated for optimal performance for each application.
* PerCP/Cyanine5.5 has a maximum absorption of 482 nm and a maximum emission of 690 nm.
- Excitation Laser
Blue Laser (488 nm)
- Application Notes
Additional reported (for relevant formats) applications include: Western Blot, blocking function2, and immunohistochemical staining of paraffin and frozen sections. The LEAF™ purified antibody is recommended for functional assays.
- Additional Product Notes
- BioLegend is in the process of converting the name PerCP/Cy5.5 to PerCP/Cyanine5.5. The dye molecule remains the same, so you should expect the same quality and performance from our PerCP/Cyanine5.5 products. Contact Technical Service if you have any questions.
(PubMed link indicates BioLegend citation)
- Takakura N, et al. 1996. J. Invest. Dermatol. 107:770.
- Liao C, et al. 2010. J. Clin. Invest. 120:242. (Block)
- Chen H, et al. 2015. ASN Neuro 8:7. PubMed.
- Miyawaki T, et al. 2004. J Neurosci. 24(37): 8124-34. (IHC-F)
AB_2715986 (BioLegend Cat. No. 135913)
AB_2715987 (BioLegend Cat. No. 135914)
- Alpha chain of the platelet-derived growth factor receptor, a receptor tyrosine kinase that forms homo- or hetero-dimers on the surface after ligand binding.
Expressed on embryonic tissues and mesenchymal-derived cells of adult mouse.
- Play a role in wound healing and act as a chemoattractant for fibroblasts, smooth muscle cells, glial cells, monocytes and neutrophils.
- Cell Type
- Embryonic Stem Cells, Mesenchymal cells, Mesenchymal Stem Cells
- Biology Area
- Cell Biology, Immunology, Neuroscience, Neuroscience Cell Markers, Stem Cells
- Molecular Family
- CD Molecules, Cytokine/Chemokine Receptors
- Antigen References
1. Mukouyama YS, et al. 2006. Proc Natl Acad Sci USA. 103(5):1551
2. Miyawaki T, et al. 2004. J Neurosci. 24(37):8124
3. Takakura N, et al. 1997. J Histochem Cytochem. 45(6):883
- Gene ID
- 18595 View all products for this Gene ID
- View information about CD140a on UniProt.org
- How stable is PerCP/Cyanine5.5 tandem as compared to PerCP alone?
PerCP/Cyanine5.5 is quite photostable and also better than PerCP alone in withstanding fixation.
|Purified anti-mouse CD140a||APA5||FC,WB,IHC-P,IHC-F|
|PE anti-mouse CD140a||APA5||FC|
|APC anti-mouse CD140a||APA5||FC|
|Biotin anti-mouse CD140a||APA5||FC|
|PerCP/Cyanine5.5 anti-mouse CD140a||APA5||FC|
|PE/Cyanine7 anti-mouse CD140a||APA5||FC|
|Brilliant Violet 605™ anti-mouse CD140a||APA5||FC|
|TotalSeq™-A0573 anti-mouse CD140a||APA5||PG|
|Brilliant Violet 421™ anti-mouse CD140a||APA5||FC|
|PE/Cyanine5 anti-mouse CD140a||APA5||FC|
|PE/Dazzle™ 594 anti-mouse CD140a||APA5||FC|
|TotalSeq™-C0573 anti-mouse CD140a||APA5||PG|
|TotalSeq™-B0573 anti-mouse CD140a||APA5||PG|
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Compare Data Across All Formats
This data display is provided for general comparisons between formats.
Your actual data may vary due to variations in samples, target cells, instruments and their settings, staining conditions, and other factors.
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Purified anti-mouse CD140a
PE anti-mouse CD140a
APC anti-mouse CD140a
Biotin anti-mouse CD140a
PerCP/Cyanine5.5 anti-mouse CD140a
PE/Cyanine7 anti-mouse CD140a
Brilliant Violet 605™ anti-mouse CD140a
TotalSeq™-A0573 anti-mouse CD140a
Brilliant Violet 421™ anti-mouse CD140a
PE/Cyanine5 anti-mouse CD140a
PE/Dazzle™ 594 anti-mouse CD140a
TotalSeq™-C0573 anti-mouse CD140a
TotalSeq™-B0573 anti-mouse CD140a