PerCP/Cy5.5 anti-mouse PLZF Antibody

Pricing & Availability
Clone
9E12 (See other available formats)
Other Names
Promyelocytic leukemia zinc finger, Zbtb16, Zinc finger and BTB domain-containing protein 16, Zfp145
Isotype
Armenian Hamster IgG
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Product Citations
publications
9E12_PerCPCy5.5_Antibody_FC_1_092515
BALB/c thymocytes were surface stained with CD3 FITC, then fixed and permeabilized using Fixation buffer and Permeabilization wash buffer, and then were stained with PLZF ( clone 9E12) PerCP/Cy5.5 (top) or Armenian hamster IgG PerCP/Cy5.5 Isotype control (bottom)
  • 9E12_PerCPCy5.5_Antibody_FC_1_092515
    BALB/c thymocytes were surface stained with CD3 FITC, then fixed and permeabilized using Fixation buffer and Permeabilization wash buffer, and then were stained with PLZF ( clone 9E12) PerCP/Cy5.5 (top) or Armenian hamster IgG PerCP/Cy5.5 Isotype control (bottom)
  • 9E12_PerCPCy5.5_Antibody_FC_2_092515
See PerCP/Cy5.5 spectral data
Cat # Size Price Quantity Avail. Save
145807 25 µg 120€
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145808 100 µg 276€
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Description

PLZF (promyelocytic leukemia zinc finger), also known as Zbtb16, Zinc finger and BTB domain-containing protein 16, Zfp145, is a member of the BTB-POZ family of transcription factors. It was first identified in a patient with acute promyelocytic leukemia, where a reciprocal chromosomal translocation t(11;17)(q23;q21) resulted in a fusion with RARA gene encoding retinoic acid receptor alpha. Expression of this transcriptional repressor in immune cells differs between human and mouse. In humans, PLZF is expressed in CD34+ progenitor cells and in primitive multipotent hematopoietic cell lines, NK cells, γ/δ T cells, CD4+ and CD8+ T cells. It is also expressed in MR1-specific mucosal-associated invariant T cells as well as in MHC class II-restricted T cells that develop via a thymocyte-thymocyte interaction. PLZF is involved in NK cell function, cellular quiescence, and growth suppression. It also inhibits gene expression induced by retinoic acid receptor. In mice, PLZF is highly expressed in immature CD1d-resricted invariant NKT (iNKT) cells, a subset of γ/δ (Vg1.1+Vd6.3+) T cells, and non-invariant CD1d-restricted T cells. PLZF exists as a homodimer or in complex with PLZP, and has been shown to be involved in the development of NKT cells. It is also reported to be expressed in embryonic tissues, giving rise to hematopoietic progenitors.

Product Details
Technical Data Sheet (pdf)

Product Details

Reactivity
Mouse
Antibody Type
Monoclonal
Host Species
Armenian Hamster
Immunogen
Combination of peptides covering the amino, carboxyl and hinge regions of the PLZF.
Formulation
Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide.
Preparation
The antibody was purified by affinity chromatography and conjugated with PE/Cy5.5 under optimal conditions. The solution is free of unconjugated PE/Cy5.5 and unconjugated antibody.
Concentration
0.2 mg/ml
Storage & Handling
The antibody solution should be stored undiluted between 2°C and 8°C, and protected from prolonged exposure to light. Do not freeze.
Application

ICFC - Quality tested

Recommended Usage

Each lot of this antibody is quality control tested by intracellular immunofluorescent staining with flow cytometric analysis. For flow cytometric staining, the suggested use of this reagent is ≤0.06 µg per million cells in 100 µl volume. It is recommended that the reagent be titrated for optimal performance for each application.

Additional Product Notes
BioLegend is in the process of converting the name PerCP/Cy5.5 to PerCP/Cyanine5.5. The dye molecule remains the same, so you should expect the same quality and performance from our PerCP/Cyanine5.5 products. Contact Technical Service if you have any questions.
RRID
AB_2566166 (BioLegend Cat. No. 145807)
AB_2566167 (BioLegend Cat. No. 145808)

Antigen Details

Structure
BTB domain-containing
Distribution
iNKT cells, γ/δ (Vg1.1+Vd6.3+) T cells, non-invariant CD1d-restricted T cells
Function
Involved in NKT cell development
Cell Type
NKT cells, T cells, Mesenchymal Stem Cells
Biology Area
Cell Biology, Immunology, Signal Transduction, Stem Cells, Transcription Factors
Antigen References

1. Constantinides MG, et al. 2011. J. Immunol. 187:309.
2. Kovalovsky D, et al. 2008. Nat. Immunol. 9:1055.
3. Chen Z, et al. 1993. EMBO J. 12:1161.
4. Chen Z, et al. 1994. Proc. Natl. Acad. Sci. USA 91:1178.

Gene ID
235320 View all products for this Gene ID
UniProt
View information about PLZF on UniProt.org

Related FAQs

How stable is PerCP/Cy5.5 tandem as compared to PerCP alone?
PerCP/Cy5.5 is quite photostable and also better than PerCP alone in withstanding fixation.
Go To Top Version: 1    Revision Date: 09/29/2015

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*These products may be covered by one or more Limited Use Label Licenses (see the BioLegend Catalog or our website, www.biolegend.com/ordering#license). BioLegend products may not be transferred to third parties, resold, modified for resale, or used to manufacture commercial products, reverse engineer functionally similar materials, or to provide a service to third parties without written approval of BioLegend. By use of these products you accept the terms and conditions of all applicable Limited Use Label Licenses. Unless otherwise indicated, these products are for research use only and are not intended for human or animal diagnostic, therapeutic or commercial use.

 

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This data display is provided for general comparisons between formats.
Your actual data may vary due to variations in samples, target cells, instruments and their settings, staining conditions, and other factors.
If you need assistance with selecting the best format contact our expert technical support team.

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