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PE/Cyanine7 anti-mouse CD64 (FcγRI) Antibody

Pricing & Availability
Clone
X54-5/7.1 (See other available formats)
Regulatory Status
RUO
Other Names
FcRI
Isotype
Mouse IgG1, κ
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Product Citations
publications
X545-71_PECyanine7_CD64_Antibody_1_030624
C57BL/6 mouse bone marrow cells were stained with CD11b APC and CD64 (clone X54-5/7.1) PE/Cyanine7 (left) or mouse IgG1, κ PE/Cyanine7 isotype control (right). Data shown was gated on myeloid cell population.
  • X545-71_PECyanine7_CD64_Antibody_1_030624
    C57BL/6 mouse bone marrow cells were stained with CD11b APC and CD64 (clone X54-5/7.1) PE/Cyanine7 (left) or mouse IgG1, κ PE/Cyanine7 isotype control (right). Data shown was gated on myeloid cell population.
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139313 25 µg 141€
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139314 100 µg 306€
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Description

CD64 is a 72 kD single chain type I glycoprotein also known as FcγRI and FcRI. CD64 is a member of the immunoglobulin superfamily and is expressed on monocytes/macrophages, dendritic cells, and mast cells. The expression can be upregulated by IFN-γ stimulation. CD64 binds IgG immune complex. It plays a role in antigen capture, phagocytosis of IgG/antigen complexes, and antibody-dependent cellular cytotoxicity (ADCC).

Product Details
Technical Data Sheet (pdf)

Product Details

Verified Reactivity
Mouse
Antibody Type
Monoclonal
Host Species
Mouse
Immunogen
BALB/c mouse FcγRI-human IgG Fc fusion protein.
Formulation
Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide.
Preparation
The antibody was purified by affinity chromatography and conjugated with PE/Cyanine7 under optimal conditions.
Concentration
0.2 mg/ml
Storage & Handling
The antibody solution should be stored undiluted between 2°C and 8°C, and protected from prolonged exposure to light. Do not freeze.
Application

FC - Quality tested
Recommended Usage

Each lot of this antibody is quality control tested by immunofluorescent staining with flow cytometric analysis. For flow cytometric staining, the suggested use of this reagent is ≤0.5 µg per million cells in 100 µl volume. It is recommended that the reagent be titrated for optimal performance for each application.

Excitation Laser
Blue Laser (488 nm)
Green Laser (532 nm)/Yellow-Green Laser (561 nm)
Application Notes

The X54-5/7.1 antibody reacts with mouse strains carrying CD64a and b alleles but not CD64d. X54-5/7.1 recognizes a conformational determinant formed between domains 2 and 3. Additional reported application (for relevant formats) include: immunoprecipitation1, and spatial biology (IBEX)5,6. Clone X54-5/7.1 is not found to be useful for Western blots1.

Additional Product Notes

BioLegend is in the process of converting the name PE/Cy7 to PE/Cyanine7. The dye molecule remains the same, so you should expect the same quality and performance from our PE/Cyanine7 products. Please contact Technical Service if you have any questions.

Application References

(PubMed link indicates BioLegend citation)
  1. Tan PS, et al. 2003. J. Immunol. 170:2549. (IP)
  2. Ingersoll MA, et al. 2010. Blood 115:e10. (FC)
  3. Ozeri E, et al. 2012. J. Immunol. 189:146. PubMed
  4. Richardson ML, et al. 2014. PLoS Negl Trop Dis. 8:2825. PubMed
  5. Radtke AJ, et al. 2020. Proc Natl Acad Sci U S A. 117:33455-65. (SB) PubMed
  6. Radtke AJ, et al. 2022. Nat Protoc. 17:378-401. (SB) PubMed
Product Citations
  1. Koyama M, et al. 2015. J Exp Med. 212: 1303 - 1321. PubMed
  2. Gallizioli M, et al. 2020. Cell Rep. 33:108291. PubMed
  3. Philpott J, et al. 2022. Immunohorizons. 6:366. PubMed
  4. Larson-Casey JL, et al. 2021. FASEB J. 35:e21675. PubMed
  5. Böttcher JP, et al. 2018. Cell. 172:1022. PubMed
  6. Larsen SE, et al. 2021. Sci Rep. 11:9040. PubMed
  7. Mitsune A, et al. 2021. Respir Res. 22:232. PubMed
  8. Helm M, et al. 2022. Life (Basel). 12:. PubMed
  9. Atkin-Smith G, et al. 2017. Sci Rep. 7:39846. PubMed
  10. Webster HC, et al. 2020. J Immunol Methods. 112702:477. PubMed
  11. Antunes KH, et al. 2022. Front Immunol. 13:867022. PubMed
  12. Ellis G, et al. 2015. EMBO Rep. 16: 1203-1218. PubMed
  13. Fantauzzi MF, et al. 2021. ERJ Open Res. 7: . PubMed
  14. Chen L, et al. 2020. J Exp Med. 217:00:00. PubMed
  15. Affo S, et al. 2021. Cancer Cell. 39(6):866-882.e11. PubMed
  16. Hole CR, et al. 2019. Nat Commun. 10:2955. PubMed
  17. Freemerman AJ, et al. 2019. J Immunol. 202:1265. PubMed
  18. Smith AS, et al. 2022. J Immunother Cancer. 10:. PubMed
  19. Alikhanyan K, et al. 2021. Cancers (Basel). 13:. PubMed
  20. Progatzky F, et al. 2021. Nature. 599:125. PubMed
  21. Yadav P, et al. 2021. J Immunol. 206:2888. PubMed
  22. Brown CC, et al. 2020. Cell. 179(4):846-863.e24.. PubMed
  23. Wu Y, et al. 2021. PLoS Negl Trop Dis. 15:e0010050. PubMed
  24. Liu CY, et al. 2020. Cell Rep. 33:108275. PubMed
  25. Macdougall CE et al. 2018. Cell metabolism. 27(3):588-601 . PubMed
  26. Martínez‐López M et al. 2019. Immunity. 50(2):446-461 . PubMed
  27. Casulli J, et al. 2019. Nat Commun. 10:2121. PubMed
  28. Kaya B, et al. 2020. Cell Reports. 32(5):107979. PubMed
  29. Smith CA, et al. 2019. JCI Insight. 4. PubMed
  30. Luo J et al. 2018. Immunity. 49(1):107-119 . PubMed
  31. Maulloo CD, et al. 2021. Front Immunol. 12:714842. PubMed
  32. Larson-Casey JL, et al. 2021. J Biol Chem. :100810. PubMed
  33. Zheng J, et al. 2020. Nature. . PubMed
  34. Olson GS, et al. 2021. Cell Reports. 35(9):109195. PubMed
  35. Giampazolias E, et al. 2021. Cell. . PubMed
  36. Youshani AS, et al. 2019. J Neuroinflammation. 16:25. PubMed
  37. Murray MP, et al. 2022. Cell Rep. 38:110209. PubMed
  38. Chen F, et al. 2022. Cell Rep. 38:110215. PubMed
  39. Birkl D, et al. 2019. FASEB J. 33:13632. PubMed
  40. Carlson PM, et al. 2021. J Immunol. 207:720. PubMed
  41. Wong NR, et al. 2021. Immunity. 54:2072. PubMed
  42. Xu J, et al. 2020. Immunity. 53(2):371-383.e5. PubMed
  43. Clemente–Casares X, et al. 2017. Immunity. 47:974. PubMed
  44. Choi J, et al. 2020. Cell Stem Cell. 27:366. PubMed
  45. Bosteels C, et al. 2020. Immunity. 52(6):1039-1056.e9.. PubMed
  46. Rochford I, et al. 2021. Am J Physiol Lung Cell Mol Physiol. 321:L686. PubMed
  47. Srivastava S, et al. 2020. Cancer Cell. 39(2):193-208.e10. PubMed
  48. Koyama M et al. 2019. Immunity. 51(5):885-898 . PubMed
  49. Larson–Casey JL, et al. 2019. J Clin Invest. 129:4962. PubMed
  50. Wang L, et al. 2020. J Nanobiotechnology. 18:38. PubMed
  51. Rappe JCF, et al. 2021. J Exp Med. 218:. PubMed
RRID
AB_2563903 (BioLegend Cat. No. 139313)
AB_2563904 (BioLegend Cat. No. 139314)

Antigen Details

Structure
Ig superfamily, type I glycoprotein, 72 kD
Distribution

Monocytes, macrophages, mast cells, dendritic cells

Function
Phagocytosis, ADCC
Ligand/Receptor
IgG
Cell Type
Dendritic cells, Macrophages, Mast cells, Monocytes
Biology Area
Immunology, Innate Immunity
Molecular Family
CD Molecules, Fc Receptors
Gene ID
14129 View all products for this Gene ID
UniProt
View information about CD64 on UniProt.org

Related FAQs

There are no FAQs for this product.
Go To Top Version: 2    Revision Date: 12/14/2021

For Research Use Only. Not for diagnostic or therapeutic use.

 

This product is supplied subject to the terms and conditions, including the limited license, located at www.biolegend.com/terms) ("Terms") and may be used only as provided in the Terms. Without limiting the foregoing, BioLegend products may not be used for any Commercial Purpose as defined in the Terms, resold in any form, used in manufacturing, or reverse engineered, sequenced, or otherwise studied or used to learn its design or composition without express written approval of BioLegend. Regardless of the information given in this document, user is solely responsible for determining any license requirements necessary for user’s intended use and assumes all risk and liability arising from use of the product. BioLegend is not responsible for patent infringement or any other risks or liabilities whatsoever resulting from the use of its products.

 

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This data display is provided for general comparisons between formats.
Your actual data may vary due to variations in samples, target cells, instruments and their settings, staining conditions, and other factors.
If you need assistance with selecting the best format contact our expert technical support team.

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