Loading...

PE/Cyanine5 anti-human CD2 Antibody

Pricing & Availability
Clone
RPA-2.10 (See other available formats)
Regulatory Status
RUO
Workshop
IV T085
Other Names
LFA-2, T11, SRBC-R
Isotype
Mouse IgG1, κ
Ave. Rating
Submit a Review
Product Citations
publications
RPA-2dot10_PE_Cy5_053107
Human peripheral blood lymphocytes stained with RPA-2.10 PE/Cyanine5
  • RPA-2dot10_PE_Cy5_053107
    Human peripheral blood lymphocytes stained with RPA-2.10 PE/Cyanine5
Compare all formats See PE/Cyanine5 spectral data
Cat # Size Price Quantity Check Availability Save
300209 25 tests 104€
Check Availability


Need larger quantities of this item?
Request Bulk Quote
300210 100 tests 227€
Check Availability


Need larger quantities of this item?
Request Bulk Quote
Description

CD2 is a 50 kD type I transmembrane glycoprotein also known as LFA-2, T11, and sheep red blood cell receptor (SRBC-R). This immunoglobulin superfamily member is expressed on thymocytes, T lymphocytes, NK cells, and thymic B cell subsets. The major ligand for CD2 is CD58 (also known as LFA-3). CD2 has also been reported to bind CD48, CD59, and CD15. CD2 plays a critical role in alternative T cell activation, T cell signaling, and cell-cell adhesion.

Product Details
Technical Data Sheet (pdf)

Product Details

Verified Reactivity
Human, Cynomolgus, Rhesus
Reported Reactivity
African Green, Baboon, Capuchin Monkey, Chimpanzee, Pigtailed Macaque, Pig
Antibody Type
Monoclonal
Host Species
Mouse
Formulation
Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide and BSA (origin USA)
Preparation
The antibody was purified by affinity chromatography, and conjugated with PE/Cyanine5 under optimal conditions.
Concentration
Lot-specific (to obtain lot-specific concentration and expiration, please enter the lot number in our Certificate of Analysis online tool.)
Storage & Handling
The antibody solution should be stored undiluted between 2°C and 8°C, and protected from prolonged exposure to light. Do not freeze.
Application

FC - Quality tested
Recommended Usage

Each lot of this antibody is quality control tested by immunofluorescent staining with flow cytometric analysis. For flow cytometric staining, the suggested use of this reagent is 5 µl per million cells in 100 µl staining volume or 5 µl per 100 µl of whole blood.

Excitation Laser
Blue Laser (488 nm)
Green Laser (532 nm)/Yellow-Green Laser (561 nm)
Application Notes

Additional reported applications (for the relevant formats) include: immunohistochemical staining of acetone-fixed frozen tissue sections6 and blocking of T cell activation2. The Ultra-LEAF™ purified antibody (Endotoxin <0.1 EU/µg, Azide-Free, 0.2 µm filtered) is recommended for functional assays (Cat. Nos. 300239 & 300240).

Additional Product Notes
BioLegend is in the process of converting the name PE/Cy5 to PE/Cyanine5. The dye molecule remains the same, so you should expect the same quality and performance from our PE/Cyanine5 products. Please contact Technical Service if you have any questions.
Application References

(PubMed link indicates BioLegend citation)
  1. Knapp W, et al. Eds. 1989. Leucocyte Typing IV. Oxford University Press. New York.
  2. Aversa G, et al. 1987. Transplant. Proc. 19:277. (Block)
  3. Zaretsky AG, et al. 2009. J. Exp Med. 206:991. (IHC) PubMed
  4. Perona-Wright G, et al. 2010. Nat. Immunol. 11:520. (FC) PubMed
  5. Thummler K, et al. 2010. J. Leukoc. Biol. 88:1041.
  6. Kap Y, et al. 2009. J. Histochem. Cytochem. 57:1159. (IHC)
  7. Yoshino N, et al. 2000. Exp. Anim. (Tokyo) 49:97. (FC)
Product Citations
  1. Natsume A, et al. 2009. Cancer Sci. 100:2411. PubMed
  2. Jung J, et al. 2019. Cell Rep. 26:1906. PubMed
  3. Bill M, et al. 2018. J Cell Mol Med. 22:2311. PubMed
  4. Gupta R, et al. 2020. Cell Stem Cell. 26:527. PubMed
  5. Rodriguez‐Meira A et al. 2019. Molecular cell. 73(6):1292-1305 . PubMed
RRID
AB_314033 (BioLegend Cat. No. 300209)
AB_314034 (BioLegend Cat. No. 300210)

Antigen Details

Structure
Ig superfamily, type I transmembrane glycoprotein, 50 kD
Distribution

T cells, NK cells, thymocytes, and thymic B cell subsets

Function
T cell activation, adhesion
Ligand/Receptor
CD58 (LFA-3), CD48, CD59, CD15
Cell Type
B cells, NK cells, T cells, Thymocytes
Biology Area
Costimulatory Molecules, Immunology
Molecular Family
Adhesion Molecules, CD Molecules
Antigen References

1. Bell G, et al. 1995. J. Immunol. 155:2805.
2. Bierer B, et al. 1989. Annu. Rev. Immunol. 7:579.
3. Moingeon P, et al. 1989. Immunol. Rev. 111:111.

Gene ID
914 View all products for this Gene ID
UniProt
View information about CD2 on UniProt.org

Related FAQs

There are no FAQs for this product.
Go To Top Version: 1    Revision Date: 11/30/2012

For research use only. Not for diagnostic use. Not for resale. BioLegend will not be held responsible for patent infringement or other violations that may occur with the use of our products.

 

*These products may be covered by one or more Limited Use Label Licenses (see the BioLegend Catalog or our website, www.biolegend.com/ordering#license). BioLegend products may not be transferred to third parties, resold, modified for resale, or used to manufacture commercial products, reverse engineer functionally similar materials, or to provide a service to third parties without written approval of BioLegend. By use of these products you accept the terms and conditions of all applicable Limited Use Label Licenses. Unless otherwise indicated, these products are for research use only and are not intended for human or animal diagnostic, therapeutic or commercial use.

 

8999 BioLegend Way, San Diego, CA 92121 www.biolegend.com
Toll-Free Phone: 1-877-Bio-Legend (246-5343) Phone: (858) 768-5800 Fax: (877) 455-9587

This data display is provided for general comparisons between formats.
Your actual data may vary due to variations in samples, target cells, instruments and their settings, staining conditions, and other factors.
If you need assistance with selecting the best format contact our expert technical support team.

  • Biotin anti-human CD2

  • FITC anti-human CD2

  • PE anti-human CD2

  • PE/Cyanine5 anti-human CD2

  • Purified anti-human CD2

  • APC anti-human CD2

  • PerCP/Cyanine5.5 anti-human CD2

  • Brilliant Violet 510™ anti-human CD2

  • APC/Cyanine7 anti-human CD2

  • PE/Cyanine7 anti-human CD2

  • Brilliant Violet 605™ anti-human CD2

  • PE/Dazzle™ 594 anti-human CD2

  • APC/Fire™ 750 anti-human CD2

  • Brilliant Violet 421™ anti-human CD2

  • Pacific Blue™ anti-human CD2

  • Alexa Fluor® 700 anti-human CD2

  • Brilliant Violet 711™ anti-human CD2

  • Brilliant Violet 785™ anti-human CD2

  • Ultra-LEAF™ Purified anti-human CD2
ProductsHere
Login / Register
Remember me
Forgot your password? Reset password?
Create an Account