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PE anti-rat CD8a Antibody

Pricing & Availability
Clone
G28 (See other available formats)
Regulatory Status
RUO
Other Names
CD8a, T8, Leu-8, Ly-2, Lyt2
Isotype
Mouse IgG2a, κ
Ave. Rating
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Product Citations
publications
G28_PE_021308
LOU rat splenocytes stained with G28 PE
  • G28_PE_021308
    LOU rat splenocytes stained with G28 PE
See PE spectral data
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200607 50 µg 94€
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200608 200 µg 259€
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Description

CD8a is a 32 kD glycoprotein also known as T8, Lyt2, Ly-2, and CD8α. CD8a is a member of the immunoglobulin superfamily expressed on most thymocytes, subset of mature T cells, most NK cells, macrophages, and some activated CD4+ T cells (not resting). CD8a forms heterodimers with the CD8β chain (CD8b) on the surface of most thymocytes, while mature peripheral T lymphocytes express almost exclusively the CD8 αβ heterodimer. Intestinal intraepithelial lymphocytes express CD8a without CD8b. CD8 is an antigen co-receptor on T cells that interacts with MHC class I on antigen-presenting cells or epithelial cells. CD8 participates in T cell activation through its association with the T cell receptor complex and protein tyrosine kinase lck (p56lck).

Product Details
Technical Data Sheet (pdf)

Product Details

Verified Reactivity
Rat
Antibody Type
Monoclonal
Host Species
Mouse
Immunogen
CD8+ Wistar rat splenic T cell hybridomas
Formulation
Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide.
Preparation
The antibody was purified by affinity chromatography, and conjugated with PE under optimal conditions.
Concentration
0.2 mg/ml
Storage & Handling
The antibody solution should be stored undiluted between 2°C and 8°C, and protected from prolonged exposure to light. Do not freeze.
Application

FC - Quality tested
Recommended Usage

Each lot of this antibody is quality control tested by immunofluorescent staining with flow cytometric analysis. For flow cytometric staining, the suggested use of this reagent is ≤0.25 µg per million cells in 100 µl volume. It is recommended that the reagent be titrated for optimal performance for each application.

Excitation Laser
Blue Laser (488 nm)
Green Laser (532 nm)/Yellow-Green Laser (561 nm)
Application Notes

Additional reported applications (for the relevant formats) include: immunoprecipitation1, blocking1 of allogeneic responses, and immunohistochemical staining of acetone-fixed frozen sections. The LEAF™ purified antibody (Endotoxin <0.1 EU/μg, Azide-Free, 0.2 μm filtered) is recommended for functional assays (Cat. No. 200612).

Application References

(PubMed link indicates BioLegend citation)
  1. Torres-Nagel N, et al. 1992. Eur. J. Immunol. 22:2841.
  2. Xie L, et al. 2014. J Immunol. 192:6009. PubMed
Product Citations
  1. Xie L, et al. 2014. J Immunol. 192:6009. PubMed
  2. Calik M, et al. 2015. ASN Neuro. 7: 1759091415595726. PubMed
  3. Zhang HC, et al. 2018. Int J Mol Med. 41:13. PubMed
RRID
AB_313878 (BioLegend Cat. No. 200607)
AB_313879 (BioLegend Cat. No. 200608)

Antigen Details

Structure
Ig superfamily, CD8α chain
Distribution
Most thymocytes, T cell subset; activated CD4+ T cells (not resting), most NK cells and intestinal intraepithelial cells express the CD8α chain without the CD8β chain
Function
Co-receptor for TCR
Ligand/Receptor
MHC class I molecule
Cell Type
Thymocytes, T cells, NK cells, Epithelial cells
Biology Area
Immunology
Molecular Family
CD Molecules
Antigen References

1. Brideau RJ, et al. 1980. Eur. J. Immunol. 10:609.
2. Dallman MJ, et al. 1982. Eur. J. Immunol. 12:511.
3. Cantrell DA, et al. 1982. Immunol. 45:97.
4. Lyscom N, et al. 1982. Immunol. 45:775.
5. Johnson P, et al. 1985. EMBO. J. 4:2539.
6. Barclay AN. 1981. Immunol. 42:593.

Gene ID
24930 View all products for this Gene ID
UniProt
View information about CD8alpha on UniProt.org

Related FAQs

What type of PE do you use in your conjugates?
We use R-PE in our conjugates.
Go To Top Version: 1    Revision Date: 11/30/2012

For Research Use Only. Not for diagnostic or therapeutic use.

 

This product is supplied subject to the terms and conditions, including the limited license, located at www.biolegend.com/terms) ("Terms") and may be used only as provided in the Terms. Without limiting the foregoing, BioLegend products may not be used for any Commercial Purpose as defined in the Terms, resold in any form, used in manufacturing, or reverse engineered, sequenced, or otherwise studied or used to learn its design or composition without express written approval of BioLegend. Regardless of the information given in this document, user is solely responsible for determining any license requirements necessary for user’s intended use and assumes all risk and liability arising from use of the product. BioLegend is not responsible for patent infringement or any other risks or liabilities whatsoever resulting from the use of its products.

 

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This data display is provided for general comparisons between formats.
Your actual data may vary due to variations in samples, target cells, instruments and their settings, staining conditions, and other factors.
If you need assistance with selecting the best format contact our expert technical support team.

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