PE anti-rat CD86 Antibody

Pricing & Availability
24F (See other available formats)
Regulatory Status
Other Names
B7-2, B70
Mouse IgG1, κ
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Product Citations
LPS-stimulated (3 days) LOU rat splenocytes stained with 24F PE
  • 24F_PE_082109
    LPS-stimulated (3 days) LOU rat splenocytes stained with 24F PE
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200307 50 µg 88€
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200308 200 µg 196€
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CD86 is an 80 kD member of the immunoglobulin superfamily also known as B70 or B7-2. It is expressed on antigen presenting cells and activated T and B cells. CD86 is a ligand for CD28 and CD152 (CTLA-4). It is one of the accessory molecules that plays an important role in T cell-APC cell costimulatory interactions. The 24F antibody can block the co-stimulatory activity of CD86.

Product Details
Technical Data Sheet (pdf)

Product Details

Verified Reactivity
Antibody Type
Host Species
HTLV-1-transformed Lewis-S1 rat T cell line
Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide.
The antibody was purified by affinity chromatography, and conjugated with PE under optimal conditions.
0.2 mg/ml
Storage & Handling
The antibody solution should be stored undiluted between 2°C and 8°C, and protected from prolonged exposure to light. Do not freeze.

FC - Quality tested

Recommended Usage

Each lot of this antibody is quality control tested by immunofluorescent staining with flow cytometric analysis. For flow cytometric staining, the suggested use of this reagent is ≤ 0.25 µg per 106 cells in 100 µl volume. It is recommended that the reagent be titrated for optimal performance for each application.

Excitation Laser
Blue Laser (488 nm)
Green Laser (532 nm)/Yellow-Green Laser (561 nm)
Application Notes

Additional reported applications (for the relevant formats) include: immunoprecipitation1-3, blocking of costimulation of T cells1-3, and immunohistochemical staining of acetone-fixed frozen sections3. The LEAF™ purified antibody (Endotoxin <0.1 EU/µg, Azide-Free, 0.2 µm filtered) is recommended for functional assays (Cat. No. 200310).

Application References

(PubMed link indicates BioLegend citation)
  1. Maeda K, et al. 1997. Int. Immunol. 9:993. (IP, Block)
  2. Bluestone JA. 1995. Immunity 2:555. (IP, Block)
  3. Damoiseaux JG, et al. 1998. J. Leuk. Biol. 64:803. (IP, Block, IHC)
  4. Donius LR, et al. 2014. Immunobiology. 219:440. PubMed
Product Citations
  1. Zonneveld-Huijssoon E, et al. 2012. Ann Rheum Dis. 71:1706. PubMed
  2. Li J, et al. 2022. Am J Transl Res. 14:5003. PubMed
  3. Fu Y, et al. 2020. Mol Ther. 1214:28. PubMed
  4. Wu H, et al. 2016. Sci Rep. 6: 24548. PubMed
  5. Martín-Fernández B, et al. 2016. PLoS One. 11: 0145946. PubMed
  6. Snell LM, et al. 2018. Immunity. 49:678. PubMed
  7. Li Y, et al. 2020. Front Immunol. 1.577083333. PubMed
  8. Lin ZH, et al. 2018. Front Cell Neurosci. 0.729861111. PubMed
  9. Wang N, et al. 2018. Exp Ther Med. 15:1906. PubMed
  10. Xin L, et al. 2022. Adv Healthc Mater. 11:e2201680. PubMed
  11. Treacy O, et al. 2012. PLoS One. 7:e42662. PubMed
AB_313856 (BioLegend Cat. No. 200307)
AB_313857 (BioLegend Cat. No. 200308)

Antigen Details

Ig superfamily, 80 kD

Antigen presenting cells, activated B cells and T cells

T and B cell costimulation
CD28, CD152 (CTLA-4)
Cell Type
B cells, T cells, Antigen-presenting cells
Biology Area
Cell Biology, Immunology, Neuroscience, Neuroscience Cell Markers, Costimulatory Molecules
Molecular Family
CD Molecules, Immune Checkpoint Receptors
Antigen References

1. Barclay AN, et al. 1997. The Leukocyte Antigen FactsBook. Academic Press.
2. Hathcock KS, et al. 1993. Science 262:905.
3. Freeman GJ, et al. 1993. Science 262:907.
4. Carreno BM, et al. 2002. Ann. Rev. Immunol. 20:29.

Gene ID
56822 View all products for this Gene ID
View information about CD86 on

Related FAQs

What type of PE do you use in your conjugates?
We use R-PE in our conjugates.
Go To Top Version: 1    Revision Date: 11/30/2012

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*These products may be covered by one or more Limited Use Label Licenses (see the BioLegend Catalog or our website, BioLegend products may not be transferred to third parties, resold, modified for resale, or used to manufacture commercial products, reverse engineer functionally similar materials, or to provide a service to third parties without written approval of BioLegend. By use of these products you accept the terms and conditions of all applicable Limited Use Label Licenses. Unless otherwise indicated, these products are for research use only and are not intended for human or animal diagnostic, therapeutic or commercial use.


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This data display is provided for general comparisons between formats.
Your actual data may vary due to variations in samples, target cells, instruments and their settings, staining conditions, and other factors.
If you need assistance with selecting the best format contact our expert technical support team.

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