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PE anti-mouse CD185 (CXCR5) Antibody

Pricing & Availability
Clone
L138D7 (See other available formats)
Regulatory Status
RUO
Other Names
BLR1, GPCR6, MDR-15
Isotype
Rat IgG2b, κ
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Product Citations
publications
L138D7_PE_CXCR5_Antibody_FC_1_040213
C57BL/6 mouse splenocytes were stained with CD45R/B220 FITC and CXCR5 (clone L138D7) PE (top) or rat IgG2b, κ PE isotype control (bottom).
  • L138D7_PE_CXCR5_Antibody_FC_1_040213
    C57BL/6 mouse splenocytes were stained with CD45R/B220 FITC and CXCR5 (clone L138D7) PE (top) or rat IgG2b, κ PE isotype control (bottom).
  • L138D7_PE_CXCR5_Antibody_FC_2_040213
Compare all formats See PE spectral data
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145503 25 µg 123€
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145504 100 µg 244€
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Description

CD185 is also known as CXCR5. It is the receptor for chemokine CXCL13/BLC, which is chemotactic for B cells.  CXCR5 is expressed on B cells and a subset of T cells in the spleen, neuronal tissue, lymph nodes, and bone marrow.  It is important for migration of B cells into the B cell follicles of the spleen and Peyer’s patches.  Follicular helper T cells (Tfh) also express CXCR5 and the ability of these cells to migrate to the lymph node is modulated by the balanced expression of CCR7 and CXCR5.

Product Details
Technical Data Sheet (pdf)

Product Details

Verified Reactivity
Mouse
Antibody Type
Monoclonal
Host Species
Rat
Immunogen
mCXCR5-transfected cells
Formulation
Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide.
Preparation
The antibody was purified by affinity chromatography and conjugated with PE under optimal conditions.
Concentration
0.2 mg/ml
Storage & Handling
The antibody solution should be stored undiluted between 2°C and 8°C, and protected from prolonged exposure to light. Do not freeze.
Application

FC - Quality tested
Recommended Usage

Each lot of this antibody is quality control tested by immunofluorescent staining with flow cytometric analysis. For flow cytometric staining, the suggested use of this reagent is ≤0.5 µg per million cells in 100 µl volume. It is recommended that the reagent be titrated for optimal performance for each application.

Excitation Laser
Blue Laser (488 nm)
Green Laser (532 nm)/Yellow-Green Laser (561 nm)
Application Notes

Clone L138D7 staining works optimally at room temperature or 4°C. Unlike other chemokine receptor antibodies, avoid using L138D7 at 37°C.

Product Citations
  1. Silva M, et al. 2021. Sci Immunol. 6:eabf1152. PubMed
  2. He W et al. 2018. Immunity. 49(6):1175-1190 . PubMed
  3. Khiew SH, et al. 2020. J Clin Invest. 130:3453. PubMed
  4. Rios D, et al. 2015. Mucosal Immunol. 101038/mi. PubMed
  5. Kurniawan H, et al. 2020. Cell Metabolism. 31(5):920-936. PubMed
  6. Trefzer A, et al. 2021. Cell Reports. 34(6):108748. PubMed
  7. Martínez‐López M et al. 2019. Immunity. 50(2):446-461 . PubMed
  8. Xing Y, et al. 2022. Cancer Lett. 529:100. PubMed
  9. Frost JN, et al. 2021. Med (N Y). 2:164. PubMed
  10. Tong D, et al. 2020. Protein Cell. 0.533333333. PubMed
  11. Di Pilato M, et al. 2021. Cell. 184(17):4512-4530.e22. PubMed
RRID
AB_2561967 (BioLegend Cat. No. 145503)
AB_2561968 (BioLegend Cat. No. 145504)

Antigen Details

Structure
42 kD, seven transmembrane protein
Distribution

B cells and a subset of T cells in mouse spleen, Tfh cells, also expressed in lymph node, bone marrow, and brain

Function
B cell migration into follicles, possible regulatory role in B cell differentiation and Burkitt's lymphoma
Ligand/Receptor
BLC/CXCL13
Cell Type
B cells, T cells, Tfh
Biology Area
Cell Biology, Immunology, Innate Immunity, Neuroinflammation, Neuroscience
Molecular Family
CD Molecules, Cytokine/Chemokine Receptors, GPCR
Antigen References

1. Kaiser E, et al. 1993. Eur. J. Immunol. 23:2532.
2. Forster R, et al. 1994. Cell. Mol. Biol. 40:381.
3. Forster R, et al. 1994. Blood 84:830.
4. Forster R, et al. 1996. Cell 87:1037.
5. Wolf I, et al. 1998. J. Biol. Chem. 273:28831.

Gene ID
12145 View all products for this Gene ID
UniProt
View information about CD185 on UniProt.org

Related FAQs

What type of PE do you use in your conjugates?
We use R-PE in our conjugates.
Go To Top Version: 1    Revision Date: 04/05/2013

For research use only. Not for diagnostic use. Not for resale. BioLegend will not be held responsible for patent infringement or other violations that may occur with the use of our products.

 

*These products may be covered by one or more Limited Use Label Licenses (see the BioLegend Catalog or our website, www.biolegend.com/ordering#license). BioLegend products may not be transferred to third parties, resold, modified for resale, or used to manufacture commercial products, reverse engineer functionally similar materials, or to provide a service to third parties without written approval of BioLegend. By use of these products you accept the terms and conditions of all applicable Limited Use Label Licenses. Unless otherwise indicated, these products are for research use only and are not intended for human or animal diagnostic, therapeutic or commercial use.

 

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This data display is provided for general comparisons between formats.
Your actual data may vary due to variations in samples, target cells, instruments and their settings, staining conditions, and other factors.
If you need assistance with selecting the best format contact our expert technical support team.

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