PE anti-human IL-1α Antibody

Product Details

Verified Reactivity

Human

Reported Reactivity

Rhesus

Antibody Type

Monoclonal

Host Species

Mouse

Immunogen

Recombinant human IL-1α

Formulation

test size: Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide and BSA (origin USA).
µg size: Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide.

Preparation

The antibody was purified by affinity chromatography, and conjugated with PE under optimal conditions.

Concentration

µg sizes: 0.2 mg/mL
test sizes: lot-specific (to obtain lot-specific concentration, please enter the lot number in our Concentration and Expiration Lookup or Certificate of Analysis online tools.)

Storage & Handling

The antibody solution should be stored undiluted between 2°C and 8°C, and protected from prolonged exposure to light. Do not freeze.

Application

ICFC - Quality tested

Recommended Usage

Each lot of this antibody is quality control tested by intracellular immunofluorescent staining with flow cytometric analysis. For flow cytometric staining, the suggested use of this reagent is 5 µl per million cells in 100 µl staining volume or 5 µl per 100 µl of whole blood.

Excitation Laser

Blue Laser (488 nm)
Green Laser (532 nm)/Yellow-Green Laser (561 nm)

Application Notes

ELISA or ELISPOT Capture¹: The purified 364-3B3-14 antibody is useful as the capture antibody in a sandwich ELISA assay, when used in conjunction with the biotinylated Poly5313 antibody (Cat. No. 531304) as the detecting antibody and recombinant human IL-1a (Cat. No. 570009) as the standard. The Ultra-LEAF™ purified antibody (Cat. No. 500108) is suggested for ELISPOT capture.
Flow Cytometry⁴: The fluorochrome-labeled 364-3B3-14 antibody is useful for intracellular immunofluorescent staining and flow cytometric analysis to identify IL-1a -producing cells within mixed cell populations. For intracellular cytokine staining protocol, please visit www.biolegend.com and click on the support section.
Additional reported applications (for the relevant formats) include: immunohistochemistry³. The 364-3B3-14 is a non-neutralizing antibody.

Application References

(PubMed link indicates BioLegend citation)

1. Thorpe R, et al. 1988. Lymphokine Res. 2:119.
2. Prussin C, et al. 1995. J. Immunol. Meth. 188:117.
3. Andersson U, et al. 1999. Detection and quantification of gene expression. New York:Springer-Verlag.
4. Enriquez, J, et al. 2002. Adv. Perit Dial. 18:177.
5. Lu Y, et al. 2015. PNAS. 112:607. PubMed

Product Citations

1. Aouba A, et al. 2010. Blood. 116:4070. PubMed

RRID

AB_315086 (BioLegend Cat. No. 500107)
AB_315085 (BioLegend Cat. No. 500106)

Antigen Details

Structure

Cytokine; 17.5 kD (Mammalian)

Bioactivity

Stimulates T cells, B cells, proliferation/activation of NK cells, fibroblasts, thymocytes, glioblastoma cells, astroglia, microglia

Cell Sources

Monocytes, tissue macrophages, Langerhans cells, dendritic cells, T and B cells, natural killer (NK) cells, large granular lymphocytes (LGL), vascular endothelium, smooth muscle, fibroblasts, thymic epithelia, astrocytes, microglia, glioma, keratinocytes,

Cell Targets

B cells, T cells, monocytes, dendritic cells

Receptors

Type I IL-1R (CDw121a), Type II IL-1R (CDw121b)

Biology Area

Cell Biology, Immunology, Innate Immunity, Neuroinflammation, Neuroscience

Molecular Family

Cytokines/Chemokines

Antigen References

1. Fitzgerald, K., et al. Eds. 2001. The Cytokine FactsBook. Academic Press, San Diego.
2. Bomford, R., et al. Eds. 1989. Interleukin-1, inflammation and disease. Elsevier, New York.
3. Brazel, D., et al. 1991. Biotechnol. Ther. 2:241.
4. Dinarello, C. 1996. Blood 87:2095.

Regulation

Upregulated by TNF-α, IFN-α, IFN-β, IFN-γ, bacterial endotoxins, viruses, mitogens, antigens; downregulated by IL-6, lipoproteins, lipids, and α₂-macroglobulin

Gene ID

3552 View all products for this Gene ID

UniProt

View information about IL-1alpha on UniProt.org

Documentation

• Technical Data Sheet (pdf)
• Certificate of Analysis
• Safety Data Sheet

Reviews

Related Pages & Pathways

Related FAQs

What type of PE do you use in your conjugates?

We use R-PE in our conjugates.

Other Formats

Concentration & Expiration Lookup

Certificate of Analysis