PE anti-human CD45 Antibody

Pricing & Availability
2D1 (See other available formats)
Regulatory Status
Other Names
Leukocyte Common Antigen (LCA), T200
Mouse IgG1, κ
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Product Citations
Human peripheral blood lymphocytes were stained with CD45 (clone 2D1) PE (filled histogram) or mouse IgG1, κ PE isotype control (open histogram).
  • 2D1_PE_CD45_Antibody_FC_102215
    Human peripheral blood lymphocytes were stained with CD45 (clone 2D1) PE (filled histogram) or mouse IgG1, κ PE isotype control (open histogram).
Compare all formats See PE spectral data
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368509 25 tests 49€
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368510 100 tests 111€
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CD45 is a 180 - 240 kD single chain type I membrane glycoprotein also known as leukocyte common antigen (LCA) and T200. It is a tyrosine phosphatase expressed on the plasma membrane of all hematopoietic cells, except erythrocytes or platelets. CD45 is a signaling molecule that regulates a variety of cellular processes including cell growth, differentiation, cell cycle, and oncogenic transformation. CD45 plays a critical role in T and B cell antigen receptor-mediated activation by dephosphorylating substrates including p56Lck, p59Fyn, and other Src family kinases. CD45 non-covalently associates with lymphocyte phosphatase-associated phosphoprotein (LPAP) on T and B lymphocytes. CD45 has been reported to bind galectin-1 and to be associated with several other cell surface antigens including CD1, CD2, CD3, and CD4.

Product Details
Technical Data Sheet (pdf)

Product Details

Verified Reactivity
Antibody Type
Host Species
Human PBMC
Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide and BSA (origin USA)
The antibody was purified by affinity chromatography and conjugated with PE under optimal conditions.
Lot-specific (to obtain lot-specific concentration and expiration, please enter the lot number in our Certificate of Analysis online tool.)
Storage & Handling
The antibody solution should be stored undiluted between 2°C and 8°C, and protected from prolonged exposure to light. Do not freeze.

FC - Quality tested

Recommended Usage

Each lot of this antibody is quality control tested by immunofluorescent staining with flow cytometric analysis. For flow cytometric staining, the suggested use of this reagent is 5 µl per million cells in 100 µl staining volume or 5 µl per 100 µl of whole blood.

Excitation Laser
Blue Laser (488 nm)
Green Laser (532 nm)/Yellow-Green Laser (561 nm)
Application Notes

It was found that the HI30 clone and the 2D1 clone can cross block each other's binding.

Application References

(PubMed link indicates BioLegend citation)
  1. Bradstock KF, et al. 1980. J. Natl. Cancer Inst. 65:33.
  2. Csiba A, et al. 1984. Br. J. Cancer 50:699.
  3. Tchilian EZ, et al. 2001. J. Immunol. 166:1308.
  4. Lee MS, et al. 2004. Int. Immunol. 16:1109.
Product Citations
  1. Sonoda S, et al. 2022. STAR Protoc. 3:101386. PubMed
  2. Paris J et al. 2019. Cell Stem Cell. 25(1):137-148 . PubMed
  3. Sluzalska K,et al. 2017. Sci Rep.. 10.1038/s41598-017-14004-9. PubMed
  4. Delvecchio FR, et al. 2021. Cell Mol Gastroenterol Hepatol. 12:1543. PubMed
  5. Dam T, et al. 2021. Front Physiol. 11:613367. PubMed
  6. Sluzalska KD, et al. 2017. Osteoarthritis Cartilage. 25:1890. PubMed
  7. Zhuang J, et al. 2020. Nucleic Acids Res. 48:8870. PubMed
  8. Morton JJ, et al. 2018. Mol Carcinog. 57:1651. PubMed
  9. Ali S, et al. 2018. Purinergic Signal. 14:371. PubMed
  10. Zhang X, et al. 2022. Nat Commun. 13:5211. PubMed
  11. Li Y, et al. 2021. Aging (Albany NY). 13:25886. PubMed
  12. Chan JM, et al. 2021. Cancer Cell. 39:1479. PubMed
  13. Cillo AR, et al. 2020. Immunity. 52(1):183-199.e9.. PubMed
  14. Liu Y, et al. 2022. iScience. 25:104405. PubMed
  15. Yu C, et al. 2020. Sci Rep. 10:14521. PubMed
  16. Yu LC, et al. 2020. Front Neurol. 1.104166667. PubMed
  17. Meng YM, et al. 2021. Nat Commun. 12:6011. PubMed
  18. Rai MA, et al. 2020. Retrovirology. 17:35. PubMed
  19. Quintanal-Villalonga A, et al. 2022. STAR Protoc. 3:101776. PubMed
  20. Tidu F, et al. 2021. iScience. 24(6):102683. PubMed
  21. Zhang Y, et al. 2018. Stem Cells Int. 2018:7159465. PubMed
  22. Chioh FW, et al. 2021. eLife. 10:00. PubMed
  23. Yao X, et al. 2022. Bioeng Transl Med. 7:e10250. PubMed
  24. Zhang H et al. 2018. Cell. 175(5):1244-1258 . PubMed
  25. Frank AC, et al. 2019. Nat Commun. 10:1135. PubMed
  26. Pan C, et al. 2019. Int J Mol Med. 44:1629. PubMed
  27. Dong N, et al. 2022. World J Stem Cells. 14:556. PubMed
  28. Wei X, et al. 2019. Int J Mol Med. 44:1425. PubMed
  29. Boya M, et al. 2022. Nat Commun. 13:3385. PubMed
AB_2566369 (BioLegend Cat. No. 368509)
AB_2566370 (BioLegend Cat. No. 368510)

Antigen Details

Type I transmembrane protein, 180 - 240 kD (multiple isoforms)

Hematopoietic cells, except circulating erythrocytes or platelets.

Tyrosine phosphatases, signaling, co-stimulation (co-inhibition), TCR and BCR mediated activation.
Galectin-1, CD2, CD3, and CD4.
Cell Type
B cells, Dendritic cells, Neutrophils
Biology Area
Cell Biology, Immunology, Inhibitory Molecules, Neuroscience, Neuroscience Cell Markers
Molecular Family
CD Molecules, Protein Kinases/Phosphatase, TCRs
Antigen References

1. Thomas M. 1989. Annu. Rev. Immunol. 7:339.
2. Trowbridge I, et al. 1994. Annu. Rev. Immunol. 12:85.

Gene ID
5788 View all products for this Gene ID
View information about CD45 on

Related FAQs

What type of PE do you use in your conjugates?
We use R-PE in our conjugates.
Go To Top Version: 1    Revision Date: 10/23/2015

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*These products may be covered by one or more Limited Use Label Licenses (see the BioLegend Catalog or our website, BioLegend products may not be transferred to third parties, resold, modified for resale, or used to manufacture commercial products, reverse engineer functionally similar materials, or to provide a service to third parties without written approval of BioLegend. By use of these products you accept the terms and conditions of all applicable Limited Use Label Licenses. Unless otherwise indicated, these products are for research use only and are not intended for human or animal diagnostic, therapeutic or commercial use.


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This data display is provided for general comparisons between formats.
Your actual data may vary due to variations in samples, target cells, instruments and their settings, staining conditions, and other factors.
If you need assistance with selecting the best format contact our expert technical support team.

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